Test:HBV Detection of Precore
Other Test Request Name:Not Applicable
Test Composition:

• Hepatitis B virus genotype

• Hepatitis B virus polymerase DNA

• Hepatitis B virus precore TAG mutation

• Hepatitis B virus basal core promoter mutation

Intended Use:

This test is used to: 
1. Identify HBV genotype (A-H) for epidemiology or prognostic purposes. 
2. Detect hepatitis B virus (HBV) mutations associated with resistance to antiviral agents.

 

 

 

Methodology:

Polymerase Chain Reaction (PCR) • Sequencing

 

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:

NOTE :

This test can only be performed reliably on specimens with a viral load of at least 600 IU/mL. 

Collection Instructions : 

Plasma (preferred): Collect blood in sterile tube containing EDTA anticoagulant; either 0.15% solution v/v final EDTA K3 (standard EDTA tube) or 9 mg spray-dried EDTA K2 (plasma preparation tube or PPT tube with plasma separator-gel, preferred). Store whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile plastic transport tube and store at -18° C or colder.

Serum (acceptable): Collect blood in sterile tube with no anticoagulants; plastic Serum Separator Tubes (SST®) are recommended. Allow blood to clot at room temperature and separate serum from cells within 2 hours of collection. Transfer serum to sterile plastic transport tube and store refrigerated or frozen. Ship frozen.

If blood is collected in a PPT tube, centrifuge preferably within 2 hours of collection as before but it is not necessary to transfer plasma to aliquot tubes. Following centrifugation, a gel barrier maintains separation of plasma from cellular components during specimen transport and storage. The PPT is plastic and hence plasma can be stored and shipped frozen in the original tube. Avoid repeated freezing and thawing of specimen. Ship frozen.

 

Collection/Sample Container:

Plasma collected in: EDTA (lavender-top) tube • Serum collected in: SST® (red-top/plastic) or no additive (red-top) tube

 

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:

4 mL Edta Plasma and 3 mL Serum

Alternative Specimen and Volume Requirement:

1 mL plasma collected in a PPT potassium EDTA (white-top) tube

 

Specimen Stability  
•   Room Temperature (15-25°C):

72 Hours

•   Refrigerated Temperature (2-8°C):

7 days

•   Freezer Temperature (-20°C):

30 days

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:

• Improperly labeled specimen

• Specimen stored outside the temperature range 

• Hemolyzed specimen

• Quantity not sufficient

• clotted specimen

Running Day:

Batch running

Cut Off Time:

Friday 4:00 PM

TAT/Releasing of Results:

14 working days after cut-off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Not Applicable

Limitations/Interferences:

None specified.

Frequently Asked Questions (FAQs):

Not applicable

Related Words/Test:Not Applicable

 

Test:HBV viral load
Other Test Request Name:

HBV DNA

Test Composition:

Not applicable

Intended Use:

Useful in quantitation of Hepatitis B Viral DNA; Useful in monitoring antiviral therapy

Methodology:

Polymerase Chain Reaction (PCR)

Laboratory Section:

Molecular Diagnostics (PCR)

Special Instructions/Patient Preparations:

No patient preparation required

Collection/Sample Container:

EDTA Tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:

1.5 - 3 mL Plasma

Alternative Specimen and Volume Requirement:

1.5 - 3 mL Serum (Serum Separator Tube)
NOTE: Plasma and Serum samples should not be used interchangeably for monitoring.

Specimen Stability  
•   Room Temperature (15-25°C):

Not specified

•   Refrigerated Temperature (2-8°C):

7 Days

•   Freezer Temperature (-20°C):

4 Weeks

Transport Temperature:

Plasma should be transported at 2 – 8 °C (with cold packs) or Frozen at -20°C to -80°C ; If whole blood will be sent in, it must be transported at 2°C – 25°C

Rejection Criteria:
  • Improper use of anticoagulant
  • Insufficient volume of blood to anticoagulant
  • Improperly labeled specimens
  • Hemolyzed sample
  • Exceeded sample stability requirement
  • Quantity not sufficient
  • Use of collection tubes containing citrate, sodium fluoride, heparin and other anticoagulants other than EDTA
Running Day:

Batch of 21 samples (Monday to Friday)

Cut Off Time:

6:00 pm

Samples receive after cut off time will be processed the next day.

TAT/Releasing of Results:

• 2 working days after receipt of specimen EXCLUDING Saturday, Sunday and Holidays
• Releasing Time: 6:00 PM

Reference Interval/Result Interpretation:

Not applicable

Limitations/Interferences:

Not applicable

Frequently Asked Questions (FAQs):

Not applicable

Related Words/Test:

Hepatitis B, HBV Tests, Hep B, Hepatitis B Virus Testing

 

Test:HCV Confirmatory
Other Test Request Name:
  • Hepatitis C confirmation
  • Immunoblot – HCV
  • Hepatitis C - Confirmation - Serum
Test Composition:

Not Applicable

Intended Use:

HCV belongs to the family of Flaviviridae and is parenterally transmitted. In France, at least 600,000 people are believed to be infected. The acute phase of the resultant hepatitis is often asymptomatic or atypical but chronic infection becomes established in over 50% of cases. Of these, one in five will develop cirrhosis which can lead to hepatocellular carcinoma.HCV represent a global public health problem. Diagnosis depends on serological analysis. A positive screen result should be confirmed using a different technique (a different, commercially available ELISA). Immunoblotting (RIBA) is of little practical relevance.

Methodology:

Immunoblot

Laboratory Section:

Special test

Special Instructions/Patient Preparations:

No patient preparation necessary.

Collection/Sample Container:

Red or Gold Tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:

3 mL Serum

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

24 Hours

•   Refrigerated Temperature (2-8°C):

5 days

•   Freezer Temperature (-20°C):

30 days

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:
  • Hemolyzed specimen
  • Markedly lipemic specimen
  • Exceeded sample stability requirement
  • Quantity not sufficient
  • Improperly labeled specimen
  • Improper collection tube used
Running Day:

Batch Running

Cut Off Time:

Friday, 12:00 PM

TAT/Releasing of Results:

3 weeks after cut off (excluding saturday, sunday and holidays)

Reference Interval/Result Interpretation:

Available upon request.

Limitations/Interferences:

None Specified.

Frequently Asked Questions (FAQs):

Not Applicable

Related Words/Test:

Not Applicable

 

Test:HCV Viral Load
Other Test Request Name:
  • HCVRNA (QUANTI)
  • HCV Quantitative Determination - Viral Load
  • VIREMIE HCV
  • Hepatitis C Viral Load
  • Viral Hepatitis C RNA by PCR
Test Composition:

Not Applicable

Intended Use:

For use in the managementof patients with chronic HCV in conjunction with clinical and laboratory markers of infection. The test can be used to predict the probability of sustained virologic response (SVR) early during a course of antiviral therapy, and to assess viral response to antiviral treatment as measured by changes of HCV RNA levles in serum or EDTA plasma.

Methodology:

Polymerase Chain Reaction(PCR)

Laboratory Section:

Molecular Diagnostics

Special Instructions/Patient Preparations:

No patient preparation required

Collection/Sample Container:

EDTA tube

Notes:

  • Follow the volume of the EDTA manufacturer's instructions for whole blood collection.
  • Whole blood must be centrifuged within 24 hours of collection.
Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:

3 mL Plasma

Alternative Specimen and Volume Requirement:

Serum(3 mL)

Specimen Stability  
•   Room Temperature (15-25°C):

Not Applicable

•   Refrigerated Temperature (2-8°C):

3 Days

•   Freezer Temperature (-20°C):

6 Weeks

Transport Temperature:
  • Plasma should be trasported at 2ºC - 10ºC (with cold packs)
  • Whole blood should be transported 2ºC - 25ºC
Rejection Criteria:
  • Improper use of anticoagulant
  • Insufficient volume of blood to anticoagulant
  • Improperly labeled specimens
  • Hemolyzed sample
  • Exceeded sample stability requirement
Running Day:

Batch running: Upon completion of Batch of 9 samples

Cut Off Time:

6:00 PM

TAT/Releasing of Results:

7 working days excluding Saturday, Sunday and Holidays

Releasing time 6:00 PM

Reference Interval/Result Interpretation:

Result: No measurable and detectable signal - HCV RNA Not Detected
Interpretation: Ct value for HCV is above the limit for the assay or no Ct value for HCV is obtained.

Result: Less than 15 IU/mL HCV RNA Detected
Interpretation: The calculated IU/mL is below the Lower Limit of Quantitation (LLOQ) of the assay.

Result: 15 upto 100,000,000 (exact value) IU/mL HCV RNA Detected
Interpretation: The calculated IU/mL is within the Linear Range of the assay. Reported result is the amount of HCV RNA detected in IU/mL.

Result: >100,000,000 IU/mL HCV RNA Detected
Interpretation: The calculated IU/mL is above the Linear Range of the assay.

Limitations/Interferences:

Not Applicable

Frequently Asked Questions (FAQs):

Q: What are the chances of someone with HCV infection developing cirrhosis or liver cancer?

A: Of every 100 people infected with HCV, approximately 5-25 will develop cirrhosis within 10-20 years. Patients who develop cirrhosis have a 1% - 4% annual risk of developing hepatocellular vcarcinoma and a 3% - 6% annual risk of hepatic decompensation.

Related Words/Test:
  • Hepatitis C
  • Viral hepatitis C Antibody Screen
  • Hepatitis C Virus

 

Test:HCV-RNA (Sequencing)- Temporarily Unavailable (currently not accepting samples)
Other Test Request Name:

Not Applicable

Test Composition:

Not Applicable

Intended Use:

To determine genotype of Hepatitis C Virus. Determining the genotype of the particular virus can be useful for predicting efficacy and for deciding which drugs and what dose to prescribe.

Methodology:

Sequencing

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:
  • No patient preparation necessary.
  • The test will only be performed if the viral load has recently been performed. Please ensure you enclose the result of the recent viral load test.
  • The recommended viral load for this test is 2000 IU/mL. However lower viral load may be tested
Collection/Sample Container:

2 pcs. 5 mL Gold or Red Tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:

10 mL of Serum

Alternative Specimen and Volume Requirement:

2pcs. of 4mL Edta or Violet top. (4mL Plasma each tube)

 

Specimen Stability  
•   Room Temperature (15-25°C):

24 Hours

•   Refrigerated Temperature (2-8°C):

5 Days

•   Freezer Temperature (-20°C):

28 Days

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:
  • Hemolyzed specimen
  • Markedly lipemic specimen
  • Exceeded sample stability requirement
  • Quantity not sufficient
  • Improperly labeled specimen
  • Improper collection tube used
Running Day:

Batch Running

Cut Off Time:

Friday, 12:00 PM

TAT/Releasing of Results:

2 months after cut-off(excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Not Applicable

Limitations/Interferences:

None Specified

Frequently Asked Questions (FAQs):

Not Applicable

Related Words/Test:

Hepatitis C Antibody, Anti-HCV, HCV-PCR, HCV-RNA, Hepatitis C Viral Load, Viral Hepatitis C Antibody Screen, Viral Hepatitis C RNA by PCR, Hepatitis C Virus Genotype

 

Test:HDL (DIRECT MEASURE- PEG)
Other Test Request Name:

• HDL-C
• High-density Lipoprotein Cholesterol

Test Composition:

Not applicable

Intended Use:

High density lipoproteins (HDL) are responsible for the reverse transport of cholesterol from the peripheral cells to the liver. Monitoring of HDL-cholesterol in serum is of clinical importance since
an inverse correlation exists between serum HDL-cholesterol concentrations and the risk of atherosclerotic disease.

Methodology:

Homogeneous enzymatic colorimetric (Direct measure- PEG)

Laboratory Section:

Chemistry

Special Instructions/Patient Preparations:

No patient preparation necessary.

Collection/Sample Container:

Red or Gold tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:

1-3 mL Serum

Alternative Specimen and Volume Requirement:

1-3mL Plasma (Li-heparin, K2 and K3 EDTA)

Specimen Stability  
•   Room Temperature (15-25°C):

3 Days

•   Refrigerated Temperature (2-8°C):

7 Days

•   Freezer Temperature (-20°C):

Serum: 12 Months; Plasma: 3 Months

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:
  • Specimens that failed the serum index criteria
  • Exceeded sample stability requirement
  • Quantity not sufficient
  • Improperly labeled specimen
  • Improper collection tube used
Running Day:

Daily

Cut Off Time:

Monday to Saturday:10:00 PM

Sunday:6:00PM

TAT/Releasing of Results:

RUNNING TIME: 6:00 AM onwards

RELEASING TIME: ROUTINE
• 4 hours after receipt of specimen/ arrival of messenger
RELEASING TIME: STAT
• After 2½ hours from extraction/messenger arrival

Reference Interval/Result Interpretation:

MALE:
>1.45:No risk for CHD,based on HDL value
0.90-1.45:Moderate risk for CHD
<0.90:High risk for CHD

FEMALE:
>1.68:No risk for CHD,based on HDL value
1.15-1.68:Moderate risk for CHD
<1.15:High risk for CHD

Limitations/Interferences:

• Elevated concentrations of free fatty acids and denatured proteins may cause falsely elevated HDL-cholesterol results.
• In rare cases, elevated immunoglobulin concentrations can lead to artificially increased HDL-cholesterol results.
• Abnormal liver function affects lipid metabolism; consequently, HDL and LDL results are of limited diagnostic value. In some patients with abnormal liver function, the HDL-cholesterol result may significantly differ from the DCM (designated comparison method) result.
• Acetaminophen intoxications are frequently treated with N-Acetylcysteine.
N-Acetylcysteine at the therapeutic concentration when used as an antidote and the Acetaminophen metabolite N-acetyl-p-benzoquinone imine (NAPQI) independently may cause falsely low results.
• Venipuncture should be performed prior to the administration of Metamizole. Venipuncture immediately after or during the administration of Metamizole may lead to falsely low results.
• In very rare cases, gammopathy, in particular type IgM (Waldenstrom’s
macroglobulinemia), may cause unreliable results.

Frequently Asked Questions (FAQs):

Q: When should testing for cholesterol/lipid profile be done?
A: In all adults aged 20 years or older, a fasting Lipid Profile should be obtained once every 5 years.

Q: Can cholesterol be done on non-fasting samples?
A: If the testing opportunity is nonfasting, only the values for total cholesterol and HDL cholesterol will be usable.

Related Words/Test:

Cholesterol, LDL Cholesterol, Triglycerides, Lipid Profile, Cardiac Risk Assessment, Lp-PLA2

 

Test:HE- Protein 4(Ovarian CA)
Other Test Request Name:

• Human Epididymal Protein 4
• HE-4

Test Composition:

Not applicable

Intended Use:
  • Used in monitoring recurrence of progressive disease in patients with epithelial ovarian cancer.
  • Used in conjunction with the CA 125 II assay as an aid in estimating the risk of epithelial ovarian cancer in premenopausal and postmenopausal women presenting with pelvic mass.
Methodology:

Electrochemiluminescence Immunoassay (ECLIA)

Laboratory Section:

Immunology

Special Instructions/Patient Preparations:

Samples should not be taken from patients receiving therapy with high biotin doses (i.e. > 5 mg/day) until at least 8 hours following the last biotin administration.

Collection/Sample Container:

Red or Gold tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:

1-3 mL Serum

Alternative Specimen and Volume Requirement:

1-3 mL Plasma (Li-heparin, K2-, K3-EDTA)

Specimen Stability  
•   Room Temperature (15-25°C):

5 Hours

•   Refrigerated Temperature (2-8°C):

48 Hours

•   Freezer Temperature (-20°C):

12 Weeks (Sample may be frozen twice)

Transport Temperature:

Transport specimen at 2°C – 8 °C (with cold packs)

Rejection Criteria:

• Hemolyzed specimen
• Markedly lipemic sample
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used

Running Day:

Batch Running (7 Days TAT)

Cut Off Time:

7:00 pm

TAT/Releasing of Results:

ROUTINE (on running days)
• 4 hours after receipt of specimen/ arrival of messenger
STAT (on running days only)
• 2½ hours from extraction/messenger arrival

Reference Interval/Result Interpretation:

<40Y: <60.50 pmol/L
40Y & 1 Day – 49Y: <76.20 pmol/L
49Y & 1 Day– 59Y: <74.30 pmol/L
59Y & 1 Day – 69Y: <82.90 pmol/L
69Y & 1 Day - 999Y: <104.00 pmol/L

Limitations/Interferences:

• Samples should not be taken from patients receiving therapy with high biotin doses (i.e. > 5 mg/day) until at least 8 hours following the last biotin administration.
• In rare cases, interference due to extremely high titers of antibodies to analyte-specific antibodies, streptavidin or ruthenium can occur.

Frequently Asked Questions (FAQs):

Not applicable

Related Words/Test:

Tumor Markers, BRCA-1 and BRCA-2, CA 125

 

Test:HealthFactr Basic
Other Test Request Name:

Not Applicable

Test Composition:
  1. Glycosylated Hb
  2. FBS
  3. Lipid Profile
  4. Algorithm by HealthFactr

 

Intended Use:

•  Assessing chronic disease risk and providing solutions

• An algorithm - based, broad spectrum health assessment tool targeting several of the most critical risk to a person’s health and longevity.

       • Results provide personalized 10-year risk scores as well as         
       recommendations  of risk mitigation. An option exists to select planned follow           up visits to monitor progress.

Methodology:

High Performance Liquid Chromatography

• HBA1c

Hexokinase

• Fasting Blood Sugar

Enzymatic Colorimetric

• GGT

• Cholesterol

• Triglyceride

Direct Measure PEG

• HDL

 

 

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:

•        10~12 hours fasting

•        Please refer to individual tests for other test-specific preparation.

 

Procedure:

 

Before HealthFactr Appointment:

1.       Hi-Precision Diagnostics updates Google calendar with member name, email, phone no.

2.       HealthFactr admin add member’s name to HF platform (name, email, gender, mobile no).

3.       Member receives activation email, resets password, agree on terms of agreement.

4.       Member completes personal profile form/page (NRIC, DOB,Ethnicity, Country of origin).

5.       Member completes Lifestyle Questionnaire (can be done at home).

 

HealthFactr Appointment Day

1.       Customer Care Specialist to verify/confirm member fills in the Patient Details and Lifestyle Questionnaire.

2.       Hi-Precision Diagnostics schedules HealthFactr appointment for member.

3.       In-House Physician logs in & access member records.

4.       In-House Physician fills in medical questionnaire & vital measurements data for member.

5.       Phlebotomist perform blood draw of member.

6.       Phlebotomist to get patient’s height and weight, pulse rate and body fat.

7.       Doctor’s appointment will then be scheduled with in-house physician as soon as laboratory results are complete.

 

HealthFactr Assessment Day

1.       After all data has been entered by doctor, laboratory test results (biomarkers) and Health Factor result will be printed by Special Test Section and released either via email or hardcopy to respective Releasing Section.

In-House Physician discuss/ explains HealthFactr and/or LiverFASt scores with patient.

Collection/Sample Container:

Gold tube (yellow top)andEDTA (violet top)

 

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:

3 mL Serumand2 mL EDTA whole blood

 

Alternative Specimen and Volume Requirement:

Not applicable

Specimen Stability  
•   Room Temperature (15-25°C):

Not applicable

•   Refrigerated Temperature (2-8°C):

7 days

•   Freezer Temperature (-20°C):

Not applicable

Transport Temperature:

Transport at 2°C~8°C (with ice packs).

 

Rejection Criteria:
  • Hemolyzed specimen
  • Quantity not sufficient
  • Incorrect collection container used
  • Improperly labeled specimen
  • Specimen stored/transported outside the temperature range
  • Laboratory tests more than 3 months ago

 

Running Day:

Batch running 

Cut Off Time:

4:00 PM

 

TAT/Releasing of Results:

10 days  after cut-off (Excluding Saturdays, Sundays and Holidays)

 

NOTES:

 

•  Available in HPD Del Monte, Fort BGC & Rockwell branches only.

Both the Laboratory (Biomarkers) Results and Health Factor result must be released to the patient.

Reference Interval/Result Interpretation:

See individual tests for reference interval.

Limitations/Interferences:

See individual tests for Limitations/Interferences.

Frequently Asked Questions (FAQs):

Q:What is HealthFCTR?

A: Algorithm that helps assess Coronary Heart Disease, Stroke, Diabetes, Liver Disease and Obstructive Sleep Apnea.

Q: Who will be interested?

  1. Insurance Companies
  2. Employers
  3. Gym facilities
  4. Health and Wellness Spas
  5. Longevity and Preventive Clinics

Q: How it works?

A:Test is very simple and quick to administer, consisting of a personal health   questionnaire, assessment/consultation with a clinician and a blood draw. Results provide personalized 10-year risk scores as well as recommendations of risk mitigation. An option exists to select planned follow up visits to monitor progress.

 

Related Words/Test:

Not applicable

 

Test:Hematocrit
Other Test Request Name:
  •  Packed Cell Volume 
  •  PCV
  • Erythrocyte Volume Fraction
  • Hct
  • Crit               
Test Composition:

Not applicable

Intended Use:

Part of Complete Blood Count that help assess patient's general health status.

Methodology:

• Hydrodynamic Focusing

Laboratory Section:

Hematology

Special Instructions/Patient Preparations:

No patient preparation necessary.

Collection/Sample Container:

EDTA (purple top)

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:

• 2mL EDTA whole blood
Note: 0.250- 0.500 mL EDTA whole blood if microtainer is used

Alternative Specimen and Volume Requirement:

Not applicable

Specimen Stability  
•   Room Temperature (15-25°C):

4 Hours

•   Refrigerated Temperature (2-8°C):

24 Hours

•   Freezer Temperature (-20°C):

Not applicable

Transport Temperature:

Transport specimen at 2 – 10 °C (with cold packs)

Rejection Criteria:

• Improperly labeled specimens
• Clotted specimens
• Over-filled or Under-filled tube
• Quantity not sufficient
• Specimen stored and transported outside the required temperature

Running Day:

Daily

Cut Off Time:

Monday to Saturday
• 6:00 PM

Sunday
• 3:00 PM

TAT/Releasing of Results:

ROUTINE
• 3 hours after receipt of specimen/ arrival of messenger
STAT
• After 1½ HOURS from extraction/messenger arrival

Reference Interval/Result Interpretation:

Available upon request.

Limitations/Interferences:

Cold agglutinins

Frequently Asked Questions (FAQs):

Not applicable

Related Words/Test:

Complete Blood Count, CBC, Hematocrit, Red blood Cell Count (RBC) , Blood Smear, Iron Tests, Reticulocytes, Vitamin B12 and Folate, Red Cell Indices, Bone Marrow Aspiration and Biopsy, G6PD, Erythropoietin, Hemoglobinopathy Evaluation, Anemia, Sickle Cell Anemia , Thalassemia, Myeloproliferative Neoplasms, Bone Marrow Disorders, Hemoglobin Abnormalities, Pregnancy.

 

Test:HEMOCHROMATOSIS C282Y
Other Test Request Name:
  • OMIM code : *613609 / #235200
  • C282Y Mutation
  • Haemochromatosis
  • c.845G>A mutation
  • Haemochromatosis-HFE gene p.Cys282Tyr mutation - whole blood
Test Composition:

Not Applicable

Intended Use:

Hereditary hemochromatosis is an autosomal recessive disease cause by deficient iron metabolism. In middle age (at between 40 and 50 years of age), untreated patients suffer serious complications including cirrhosis, diabetes, arthropathy, heart disease, bronze pigmentation of the skin, loss of pituitary function and liver cancer. If the disease is diagnosed at an early stage, its worst consequences can be avoided by periodic therapeutic phlebotomy. Genetic diagnosis is based on looking for mutations C282Y and H63D in the HFE gene on chromosome 6. Only homozygosity for the C282Y mutation establishes a definite diagnosis with the current state of understanding. If an individual is diagnosed with this mutation, it should be explained why other members of the family should be tested.

Methodology:

Real-Time Polymerase Chain Reaction (RT-PCR)

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:

Blood sample will be drawn every Thursday from 6am to 6pm

Collection/Sample Container:

EDTA or Violet Tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:

3 pcs of 4 mL EDTA whole blood

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

24 Hours

•   Refrigerated Temperature (2-8°C):

5 Days

•   Freezer Temperature (-20°C):

Not Applicable

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:
  • Over-filled or Under-filled tube
  • Clotted Specimen
  • Exceeded sample stability requirement
  • Quantity not sufficient
  • Improperly labeled specimen
  • Improper collection tube used
Running Day:

Batch Running

Cut Off Time:

Friday, 4:00 PM

TAT/Releasing of Results:

2 weeks after cut-off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Available upon request

Limitations/Interferences:

None Specified

Frequently Asked Questions (FAQs):

Not Applicable

Related Words/Test:

Iron Overload Disease; Iron Storage Disease

 

Test:Hemochromatosis H63D
Other Test Request Name:
  • Haemochromatosis
  • c.187C>G mutation
  • H63D mutation
  • Iron Overload Disease
  • Iron Storage Disease
Test Composition:

Not Applicable

Intended Use:

Hereditary hemochromatosis is an autosomal recessive disease cause by deficient iron metabolism. In middle age (at between 40 and 50 years of age), untreated patients suffer serious complications including cirrhosis, diabetes, arthropathy, heart disease, bronze pigmentation of the skin, loss of pituitary function and liver cancer. If the disease is diagnosed at an early stage, its worst consequences can be avoided by periodic therapeutic phlebotomy. Genetic diagnosis is based on looking for mutations C282Y and H63D in the HFE gene on chromosome 6. Only homozygosity for the C282Y mutation establishes a definite diagnosis with the current state of understanding. If an individual is diagnosed with this mutation, it should be explained why other members of the family should be tested.

Methodology:

Real-Time Polymerase Chain Reaction (RT-PCR)

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:
  • No patient preparation necessary.
  • Inform Special Test Section 1 day before the scheduled sample collection.
  • Ask for Specific Request Form (B12-INTGB) and Attestation Consent (D43-INTGB) at Special Test Section atleast one day prior submission of sample.
  • Completely fill-out the required information in the Submission form.
  • Blood sample will be drawn every Monday only.
  • Send sample immediately to HP Main Lab.
Collection/Sample Container:

EDTA or Violet Tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:

3 pcs of 4 mL EDTA whole blood

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

24 Hours

•   Refrigerated Temperature (2-8°C):

5 Days

•   Freezer Temperature (-20°C):

Not Applicable

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:
  • Over-filled or Under-filled tube
  • Clotted Specimen
  • Exceeded sample stability requirement
  • Quantity not sufficient
  • Improperly labeled specimen
  • Improper collection tube used
Running Day:

Batch running

Cut Off Time:

Monday, 12:00 PM

TAT/Releasing of Results:

2 weeks after cut-off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Available upon request

Limitations/Interferences:

None Specified.

Frequently Asked Questions (FAQs):

Not Applicable.

Related Words/Test:

Iron Overload Disease; Iron Storage Disease

 

Test:HEMODIALYSIS KINETICS
Other Test Request Name:

Not applicable

Test Composition:

1. Pre-BUN
2. Post-BUN
3. Post/Pre Dialysis Ratio
4. Kt/V
5. Urea Reduction Rate

Intended Use:

Used to measure dialysis adequacy.

Methodology:

Kinetic test with urease and glutamate dehydrogenase.

Laboratory Section:

Chemistry

Special Instructions/Patient Preparations:

• No patient preparation necessary.
• Provide the following details:
- Pre-dialysis weight
- Post-dialysis weight
- Ultra Filtration Volume
- Duration of Dialysis

NOTE: Do not draw specimen during hemodialysis.

Collection/Sample Container:

Red or Gold tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:

1-3 mL Serum

Alternative Specimen and Volume Requirement:

1-3mL Plasma (Li?heparin and K2?EDTA)

Specimen Stability  
•   Room Temperature (15-25°C):

7 Days

•   Refrigerated Temperature (2-8°C):

7 Days

•   Freezer Temperature (-20°C):

1 Year

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:

• Hemolyzed specimen
• Markedly lipemic specimen
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used

Running Day:

Daily

Cut Off Time:

Monday to Saturday:10:00 PM

Sunday:6:00PM

TAT/Releasing of Results:

RUNNING TIME: 6:00 AM onwards

RELEASING TIME: ROUTINE
• 4 hours after receipt of specimen/ arrival of messenger
RELEASING TIME: STAT
• After 2½ hours from extraction/messenger arrival

Reference Interval/Result Interpretation:

0-3 y/o: 1.79-6.43 mmol/L (5.01-17.00 mg/dL)
3Y & 1 Day - 13Y: 2.50-6.07 mmol/L (7.00-17.00 mg/dL)
13Y & 1 Day - 999Y: 2.18-8.33 mmol/L (6.10-23.32 mg/dL)

Limitations/Interferences:

• Ammonium ions may cause erroneously elevated results.
• In very rare cases, gammopathy, in particular type IgM (Waldenstrom’s macroglobulinemia), may cause unreliable results.

Frequently Asked Questions (FAQs):

Not applicable

Related Words/Test:

Kidney Function, Dialysis

 

Test:Hemoglobin
Other Test Request Name:

• Hgb
• Hb

Test Composition:

Not applicable

Intended Use:

Part of Complete Blood Count that help assess patient's general health status.

Methodology:

• Sodium Lauryl Sulfate (SLS) Method)

Laboratory Section:

Hematology

Special Instructions/Patient Preparations:

No patient preparation necessary.

Collection/Sample Container:

EDTA (purple top)

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:

• 2mL EDTA whole blood
Note: 0.250- 0.500 mL EDTA whole blood if microtainer is used

Alternative Specimen and Volume Requirement:

Not applicable

Specimen Stability  
•   Room Temperature (15-25°C):

4 Hours

•   Refrigerated Temperature (2-8°C):

24 Hours

•   Freezer Temperature (-20°C):

Not applicable

Transport Temperature:

Transport specimen at 2 – 10 °C (with cold packs)

Rejection Criteria:

• Improperly labeled specimens
• Clotted specimens
• Over-filled or Under-filled tube
• Quantity not sufficient
• Specimen stored and transported outside the required temperature

Running Day:

Daily

Cut Off Time:

Monday to Saturday
• 6:00 PM

Sunday
• 3:00 PM

TAT/Releasing of Results:

ROUTINE
• 3 hours after receipt of specimen/ arrival of messenger
STAT
• After 1½ HOURS from extraction/messenger arrival

Reference Interval/Result Interpretation:

Available upon request.

Limitations/Interferences:

Cold agglutinins

Frequently Asked Questions (FAQs):

Not applicable

Related Words/Test:

Complete Blood Count, CBC, Hematocrit, Red blood Cell Count (RBC) , Blood Smear, Iron Tests, Reticulocytes, Vitamin B12 and Folate, Red Cell Indices, Bone Marrow Aspiration and Biopsy, G6PD, Erythropoietin, Hemoglobinopathy Evaluation, Anemia, Sickle Cell Anemia, Thalassemia, Myeloproliferative Neoplasms, Bone Marrow Disorders, Hemoglobin Abnormalities, Pregnancy.

 

Test:Hemoglobin & Hematocrit
Other Test Request Name:

H and H

Test Composition:

1. Hemoglobin
2. Hematocrit

Intended Use:

Part of Complete Blood Count that help assess patient's general health status.

Methodology:

• Hemoglobin: Sodium Lauryl Sulfate (SLS) Method)
• Hematocrit: Hydrodynamic Focusing

Laboratory Section:

Hematology

Special Instructions/Patient Preparations:

No patient preparation necessary.

Collection/Sample Container:

EDTA (purple top)

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:

• 2mL EDTA whole blood
Note: 0.250- 0.500 mL EDTA whole blood if microtainer is used

Alternative Specimen and Volume Requirement:

Not applicable

Specimen Stability  
•   Room Temperature (15-25°C):

4 Hours

•   Refrigerated Temperature (2-8°C):

24 Hours

•   Freezer Temperature (-20°C):

Not applicable

Transport Temperature:

Transport specimen at 2 – 10 °C (with cold packs)

Rejection Criteria:

• Improperly labeled specimens
• Clotted specimens
• Over-filled or Under-filled tube
• Quantity not sufficient
• Specimen stored and transported outside the required temperature

Running Day:

Daily

Cut Off Time:

Monday to Saturday
• 6:00 PM

Sunday
• 3:00 PM

TAT/Releasing of Results:

ROUTINE
• 3 hours after receipt of specimen/ arrival of messenger
STAT
• After 1½ HOURS from extraction/messenger arrival

Reference Interval/Result Interpretation:

Available upon request.

Limitations/Interferences:

Cold agglutinins

Frequently Asked Questions (FAQs):

Not applicable

Related Words/Test:

Complete Blood Count, CBC, Hematocrit, Red blood Cell Count (RBC), Blood Smear, Iron Tests, Reticulocytes, Vitamin B12 and Folate, Red Cell Indices, Bone Marrow Aspiration and Biopsy, G6PD, Erythropoietin, Hemoglobinopathy Evaluation, Anemia, Sickle Cell Anemia, Thalassemia, Myeloproliferative Neoplasms, Bone Marrow Disorders, Hemoglobin Abnormalities, Pregnancy.

 

Test:HEP-B AntiViralResistance- Temporarily Unavailable (currently not accepting samples)
Other Test Request Name:
HBV-anti-viral resistance profile
Test Composition:

Not Applicable

Intended Use:

To help assess treatment of Hepatitis B patient.

Methodology:

Sequencing

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:

The test will only be perform if the viral load has recently been performed. Please ensure you enclose the result of the recent viral load test.

 

The minimum viral load is 1000 copies/mL (180 IU)

Collection/Sample Container:

Red or Gold Tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:

5 mL Serum

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

Not Applicable

•   Refrigerated Temperature (2-8°C):

24 Hours

•   Freezer Temperature (-20°C):

14 Days

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:
  • Hemolyzed specimen
  • Markedly lipemic specimen
  • Exceeded sample stability requirement
  • Quantity not sufficient
  • Improperly labeled specimen
  • Improper collection tube used
Running Day:

Batch Running

Cut Off Time:

Friday, 12:00 PM

TAT/Releasing of Results:

1 month after cut-off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Not Applicable

Limitations/Interferences:

None Specified

Frequently Asked Questions (FAQs):

Not Applicable

Related Words/Test:

HBV Tests, Hep B, anti-HBs, Hepatitis B Surface Antibody, HBsAg, Hepatitis B Surface Antigen, HBeAg, Hepatitis B e Antigen, anti-HBc, Hepatitis B Core Antibody, anti-HBc, IgM, anti-Hbe, Hepatitis B e Antibody, HBV DNA, Hepatitis B Virus Testing