Laboratory Test Preparation

Test:Immunoflourescence IgA
Other Test Request Name:

Immunoglobulin A

 

Test Composition:

Not Applicable

Intended Use:

Increased IgA is associated with monoclonal IgA myeloma, respiratory and gastrointestinal infections, and malabsorption; decreased IgA is found in selective IgA deficiency and in ataxia telangiectasia.

 

Methodology:

Immunoturbidimetric

 

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:

No patient preparation.

Collection/Sample Container:

Red or Gold Tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

3 mL Serum

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

72 Hours

•   Refrigerated Temperature (2-8°C):

7 days

•   Freezer Temperature (-20°C):

90 days

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:

• Hemolyzed specimen
• Markedly lipemic specimen
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used

Running Day:

Batch Running

Cut Off Time:

Friday 4:00 PM

TAT/Releasing of Results:

14 working days after cut-off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Not Applicable

Limitations/Interferences:

None specified.

Frequently Asked Questions (FAQs):

Not applicable

Related Words/Test:Not Applicable

 

Test:Immunoglobulin A/IgA
Other Test Request Name:
  • Serum IgA
  • Immune globulin
  • Ig
  • Immune serum globulin
  • Gamma globulin
Test Composition:

Not applicable

Intended Use:

Measures antibody levels or evaluates the effectiveness of treatment.

One of the five classes of immunoglobulins; one of the most common immunoglobulins, it is mainly present in body secretions and is the chief antibody in the mucous membranes of the gastrointestinal and respiratory tract and in saliva and tears.

Methodology:

Immnoturbidimetry

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:

No patient preparation necessary.

Collection/Sample Container:

Red or Gold Tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

3 mL Serum

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

24 Hours

•   Refrigerated Temperature (2-8°C):

7 Days

•   Freezer Temperature (-20°C):

Not Applicable

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:
  • Hemolyzed specimen
  • Markedly lipemic specimen
  • Exceeded sample stability requirement
  • Quantity not sufficient
  • Improperly labeled specimen
  • Improper collection tube used
Running Day:

Monday and Thursday

Cut Off Time:
  • Friday (for Monday running), 7:00 AM
  • Wednesday (for Thursday running), 7:00 AM
TAT/Releasing of Results:

3 days after running day (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Not Applicable

Limitations/Interferences:

None specified

Frequently Asked Questions (FAQs):

Not applicable

Related Words/Test:

Total Immunoglobulins, Immunoglobulin G, IgG, Immunoglobulin M, IgM, Immunoglobulins, Quantitative

 

Test:Immunoglobulin G/IgG
Other Test Request Name:
  • Serum IgG
  • Immune globulin
  • Ig
  • Immune serum globulin
  • Gamma globulin

 

Test Composition:

Not applicable

Intended Use:

Measures antibody levels or evaluates the effectiveness of treatment.

One of the five classes of immunoglobulins; one of the most common immunoglobulins, it is mainly present in body secretions and is the chief antibody in the mucous membranes of the gastrointestinal and respiratory tract and in saliva and tears.

Methodology:

Immnoturbidimetry

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:

No patient preparation necessary.

Collection/Sample Container:

Red or Gold Tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

3 mL Serum

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

24 Hours

•   Refrigerated Temperature (2-8°C):

7 Days

•   Freezer Temperature (-20°C):

Not Applicable

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:
  • Hemolyzed specimen
  • Markedly lipemic specimen
  • Exceeded sample stability requirement
  • Quantity not sufficient
  • Improperly labeled specimen
  • Improper collection tube used
Running Day:

Monday and Thursday

Cut Off Time:
  • Friday (for Monday running), 7:00 AM
  • Wednesday (for Thursday running), 7:00 AM
TAT/Releasing of Results:

3 days after running day (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Not Applicable

Limitations/Interferences:

None specified

Frequently Asked Questions (FAQs):

Not applicable

Related Words/Test:

Total Immunoglobulins, Immunoglobulin A, IgA, Immunoglobulin M, IgM, Immunoglobulins, Quantitative

 

Test:Immunoglobulin M/ IgM
Other Test Request Name:
  • Serum IgM
  • Immune globulin
  • Ig
  • Immune serum globulin
  • Gamma globulin
Test Composition:

Not applicable

Intended Use:

Measures antibody levels or evaluates the effectiveness of treatment.

One of the five classes of immunoglobulins; one of the most common immunoglobulins, it is mainly present in body secretions and is the chief antibody in the mucous membranes of the gastrointestinal and respiratory tract and in saliva and tears.

Methodology:

Immnoturbidimetry

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:

No patient preparation necessary.

Collection/Sample Container:

Red or Gold Tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

3 mL Serum

Alternative Specimen and Volume Requirement:

Not applicable

Specimen Stability  
•   Room Temperature (15-25°C):

24 Hours

 

•   Refrigerated Temperature (2-8°C):

7 Days

•   Freezer Temperature (-20°C):

Not Applicable

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:
  • Hemolyzed specimen
  • Markedly lipemic specimen
  • Exceeded sample stability requirement
  • Quantity not sufficient
  • Improperly labeled specimen
  • Improper collection tube used
Running Day:

Monday and Thursday

Cut Off Time:
  • Friday (for Monday running), 7:00 AM
  • Wednesday (for Thursday running), 7:00 AM
TAT/Releasing of Results:

3 days after running day (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Available upon request

Limitations/Interferences:

None specified

Frequently Asked Questions (FAQs):

Not applicable

Related Words/Test:

Total Immunoglobulins, Immunoglobulin A, IgA, Immunoglobulin G, IgG, Immunoglobulins, Quantitative

 

Test:Immunotyping Electro (Urine) (BY APPOINTMENT PER PATIENT)
Other Test Request Name:

Not Applicable

Test Composition:

With Urine Total Potein

Intended Use:

Immunofixation of urine is useful in evaluation of monoclonal free light chains and other monoclonal gammopathies seen in light chain disease, multiple myeloma, Waldenstrom's macroglobulinemia, amyloidosis, and other lymphoproliferative disorders. Increased polyclonal free light chains in urine may be seen in glomerular leak syndromes and in infection or inflammation.

Methodology:

Immunofixation

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:
  • No patient preparation necessary.
  • Call Special Test Section (By Appointment/Schedule.

NOTE: Do not collect specimen without approval of Special Test Section

 

Collection Procedures :

  1. Instruct the patient to void at the beginning of collection period and discard the specimen.
  2. Collect all urine including the final specimen voided at the end of collection period.
  3. Mix 24 hour urine collected.
  4. Label the bottle with patient name, date & time collection started, date & time collection ended and total volume measured.
  5. Note total volume.
  6. Submit the specimen to HPD
Collection/Sample Container:

Plastic leak proof clean container without preservative

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

100 mL of 24 hour Urine

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

24 Hours

•   Refrigerated Temperature (2-8°C):

7 Days

•   Freezer Temperature (-20°C):

28 Days

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:
  • Quantity not sufficient
  • Received room temperature
  • Improperly labeled specimen
  • Improper urine collection
Running Day:

Mondays and Thursdays

Cut Off Time:

Thursday, 7:00 AM (Monday Running)

Monday, 7:00 AM (Thursday Running)

TAT/Releasing of Results:

7 working days after running day (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Available upon request

Limitations/Interferences:

None Specified.

Frequently Asked Questions (FAQs):

Not Applicable

Related Words/Test:

Levels of certain proteins, monoclonal immunoglobulins, multiple myeloma, Waldenstrom macroglobulinemia.

 

Test:India Ink
Other Test Request Name:India Ink (Colloidal Carbon) Stain
Test Composition:Not applicable
Intended Use:India Ink is used to detect cryptococcal meningitis. This is also requested for immunocompromised patients such as those with AIDS, disseminated infection or debilitating diseases. Test should be done even without prior request if yeast cells are seen when performing a cell count or Gram stain
Methodology:Direct Microscopic Examination using Nigrosin stain
Laboratory Section:Bacteriology
Special Instructions/Patient Preparations:Instructions to Send-in Clients:
• Indicate patient’s diagnosis, medical history and sample type on request forms.
Collection/Sample Container:Sterile leak-proof container
Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:1 - 4 mL Cerebrospinal Fluid
Alternative Specimen and Volume Requirement:Not applicable
Specimen Stability  
•   Room Temperature (15-25°C):6 Hours
•   Refrigerated Temperature (2-8°C):Not applicable
•   Freezer Temperature (-20°C):Not applicable
Transport Temperature:Transport specimens immediately (within 6 hours) to HPD Laboratory at room temperature (15-25°C)

NOTE: Do not delay transport of samples.
Rejection Criteria:• Incorrect label
• Quantity not sufficient
• Exceeded sample stability and storage requirement

Running Day:Daily
Cut Off Time:6:00 PM
TAT/Releasing of Results:ROUTINE:
• 4 hours after receipt of specimen/ arrival of messenger
STAT:
• 1½ hours from extraction/messenger arrival
Reference Interval/Result Interpretation:Not applicable
Limitations/Interferences:India ink is limited to Cerebrospinal fluid (CSF) specimens only.
Frequently Asked Questions (FAQs):Not applicable
Related Words/Test:Not applicable

 

Test:Influenza A & B Ab (CF)
Other Test Request Name:

Influenza A and B Antibodies

 

Test Composition:

Not Applicable

Intended Use:

Influenza Type A and B viruses cause seasonal outbreaks of the flu. Both Type A and B are included in the flu vaccine.

Methodology:

Complement Fixation (CF)

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:

No patient preparation necessary.

Collection/Sample Container:

Red or Gold Tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

3 mL Serum

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

24 Hours

•   Refrigerated Temperature (2-8°C):

7 Days

•   Freezer Temperature (-20°C):

30 Days

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:
  • Hemolyzed specimen
  • Markedly lipemic specimen
  • Exceeded sample stability requirement
  • Quantity not sufficient
  • Improperly labeled specimen
  • Improper collection tube used
Running Day:

Batch Running

Cut Off Time:

Monday, 12:00 PM

 

TAT/Releasing of Results:

2 weeks after cut-off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Available upon request

Limitations/Interferences:

None Specified.

Frequently Asked Questions (FAQs):

Not Applicable

Related Words/Test:

Flu Test, Rapid Flu Test, Influenza Antigen Test, H1N1, Rapid Influenza Diagnostic Test, RIDT, Flu PCR, Influenza Rapid Antigen Test, Influenza Type A and B Antigen Detection, Influenza A and B Culture, Influenza Type A H1N1, PCR for Influenza Virus

 

Test:Inhibin B (EIA)
Other Test Request Name:INH-B
Test Composition:Not applicable
Intended Use:Inhibin B is the major circulating inhibin in males. It is also detectable in women during menstrual cycles, particularly prior to ovulation. The measurement of inhibin B serves as an endocrine marker for monitoring male and female gonadal function.
Methodology:Enzyme Immunoassay (EIA)
Laboratory Section:Special Test
Special Instructions/Patient Preparations:No patient preparation necessary.
Collection/Sample Container:Red or Gold Tube
Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:3 mL Serum
Alternative Specimen and Volume Requirement:Not Applicable
Specimen Stability  
•   Room Temperature (15-25°C):72 Hours
•   Refrigerated Temperature (2-8°C):7 Days
•   Freezer Temperature (-20°C):28 Days
Transport Temperature:Transport specimen at 2 – 8 °C (with cold packs)
Rejection Criteria:• Hemolyzed specimen
• Markedly lipemic specimen
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used
Running Day:Batch Running
Cut Off Time:Friday 4:00 PM
TAT/Releasing of Results:2 weeks after cut-off (excluding Saturdays, Sundays and Holidays)
Reference Interval/Result Interpretation:Available upon request
Limitations/Interferences:None specified.
Frequently Asked Questions (FAQs):Not applicable
Related Words/Test:Spermatogenesis function and male infertility

 

Test:Insulin (ECLIA)
Other Test Request Name:

• Serum Insulin
• Fasting Insulin

Test Composition:

Not applicable

Intended Use:

The determination of insulin is utilized in the diagnosis and therapy of various disorders of carbohydrate metabolism, including diabetes mellitus and hypoglycemia.

Methodology:

Electrochemiluminescence Immunoassay (ECLIA)

Laboratory Section:

Immunology

Special Instructions/Patient Preparations:

• Samples should not be taken from patients receiving therapy with high biotin doses (i.e. > 5 mg/day) until at least 8 hours following the last biotin administration.

NOTE: Patient preparation will depend on doctor's request (fasting, after 1 hour, 2 hours, etc..). Routine fasting time is 8 hours.

Collection/Sample Container:

Red or Gold tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

1-3 mL Serum

Alternative Specimen and Volume Requirement:

1-3 mL Plasma (Li?heparin, K3 ?EDTA and Sodium Citrate)

Specimen Stability  
•   Room Temperature (15-25°C):

Not specified

•   Refrigerated Temperature (2-8°C):

24 Hours

•   Freezer Temperature (-20°C):

6 Months (Freeze only once)

Transport Temperature:

Transport specimen at 2°C – 8 °C (with cold packs)

Rejection Criteria:

• Hemolyzed specimen (Hemolysis interferes, as insulin?degrading peptidases are released from
erythrocytes)
• Markedly lipemic sample
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used

Running Day:

Monday, Wednesday, Friday

Cut Off Time:

Monday to Saturday:10:00 PM

Sunday:6:00PM

TAT/Releasing of Results:

ROUTINE (on running days)
• 4 hours after receipt of specimen/ arrival of messenger
STAT (on running days only)
• 2½ hours from extraction/messenger arrival

Reference Interval/Result Interpretation:

2.60-24.90 uU/mL

Limitations/Interferences:

• Samples should not be taken from patients receiving therapy with high biotin doses (i.e. > 5 mg/day) until at least 8 hours following the last biotin administration.
• In rare cases, interference due to extremely high titers of antibodies to analyte?specific antibodies, streptavidin or ruthenium can occur.
• Samples from patients treated with bovine, porcine or human insulin sometimes contain anti-insulin antibodies which can affect the test results.

Frequently Asked Questions (FAQs):

Not applicable

Related Words/Test:

C-peptide, Glucose Tests

 

Test:Insulin (HOMA)
Other Test Request Name:
  • Homeostatic Model Assessment Insulin Resistance
  • HOMA index IR
  • Insulin resistance
Test Composition:

Not Applicable

Intended Use:

Differential diagnosis of diabetes and investigation of pancreatic function.

Methodology:

Electrochemiluminescence

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:
  • 8-12 hours fasting
  • STOP treatment 8 days before taking the sample with patients treated with biotin (Vitamin B7, B8 or H) or taking any food supplements containing biotin.

RECEPTION/ENCODERS:

  • FBS (Fasting Blood Sugar) shall be requested and charged along with Insulin HOMA request.
  • FBS Test code shall be automatically incorporated in Web LIS for Insulin HOMA test request.

 ?    TEST CODE: HOMA9 - Insulin (HOMA) paired with FBS - FBS (HEXOKINASE) 

  • Inform the patient/client that previous FBS result is not acceptable.

BRANCHES:

  • Secondary Laboratory branch may perform the FBS test.
  • Must provide the FBS result at Clinical information field.

 

Collection/Sample Container:

Red or Gold Tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

3mL Serum

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

24 Hours

•   Refrigerated Temperature (2-8°C):

5 Days

•   Freezer Temperature (-20°C):

1 Month

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:
  • Hemolyzed sample
  • Markedly lipemic sample
  • Exceeded sample stability requirement
  • Quantity not sufficient
  • Improperly labeled sample
  • Improper collection tube used
  • Same day FBS result not provided.
Running Day:

Batch Running

Cut Off Time:

Friday, 12:00 PM

 

TAT/Releasing of Results:

2 weeks after cut off (excluding saturday, sunday and holidays)

Reference Interval/Result Interpretation:

Available Upon Request

 

Limitations/Interferences:

Interference possible in patients treated with biotin (vitamin B7, B8 or H) or taking any food supplements containing biotin.

 

Frequently Asked Questions (FAQs):

Q: What is insulin resistance?

A:Insulin resistance is classically defined as impaired whole-body insulin-mediated glucose disposal.

However, defective insulin action is not confined to impaired glucose disposal; it can also be seen in protein and fat metabolism. Therefore, a more accurate definition of insulin resistance is “a state in which normal insulin levels produce an attenuated biological response. “Improvement of insulin sensitivity/insulin action or reduction in insulin resistance, as a therapeutic principle may play a central role in the primary prevention and treatment of type 2 diabetes, hypertension, and atherosclerosis.

Q: How common is insulin resistance?

A:Insulin resistance is very common in the general population. The prevalence of insulin resistance has been estimated to be as high as 88% in people with lipid abnormalities, such as elevated triglycerides and low HDL. Considering that over half of all adults in Western populations have lipid abnormalities; it becomes clear that insulin resistance is not a problem confined to diabetes but a mass problem afflicting the vast majority. A surprising finding is that people with elevated triglycerides and low HDL can have a similar degree of insulin resistance as that seen in patients with type 2 diabetes.

Importantly, in the general population insulin resistance can be found even in the absence of any major metabolic disorders, and 25% of apparently healthy people are severely insulin resistant. This can be detected by elevated fasting insulin and glucose levels and the HOMA-IR index and may be an indicator of “silent” atherosclerosis.

Q: What is the value of assessing insulin resistance?

A:Insulin resistance is an independent risk factor for all-cause and cardiovascular mortality, and multiple studies have shown that insulin resistance is a strong predictor of atherosclerotic cardiovascular disease as well as adverse cardiovascular outcomes in both non-diabetic and diabetic individuals.

A notable study found that insulin resistance, as estimated by HOMA-IR was associated with incident symptomatic cardiovascular disease after a 15-year follow-up.

Importantly, the association of insulin resistance with cardiovascular disease was independent of classic risk factors (including hyperglycemia, hypertension, high LDL cholesterol, smoking, and physical activity) and of other components of the metabolic syndrome (obesity, hypertriglyceridemia, and low HDL cholesterol).

 

 

Related Words/Test:

Insulinaemia, Insulin, Fasting Blood Sugar

 

 

Test:Insulin (RIA) Temporarily Unavailable (currently not accepting samples)
Other Test Request Name:

Not applicable

Test Composition:

Not applicable

Intended Use:

• Diagnosis of insulinoma and other causes of hypoglycemia
• Diagnosis and study of cases of insulin resistance (obesity, various endocrinopathies, insulin receptor defects, anti-receptor antibodies)

Methodology:

Radio Immuno Assay (RIA)

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:

No patient preparation necessary.

Collection/Sample Container:

Red or Gold Tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

3 mL Serum

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

72 Hours

•   Refrigerated Temperature (2-8°C):

7 Days

•   Freezer Temperature (-20°C):

28 Days

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:

• Hemolyzed specimen
• Markedly lipemic specimen
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used

Running Day:

Wednesday 8:00 AM

Cut Off Time:

Tuesday 4:00 PM

TAT/Releasing of Results:

After 3 days (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Available upon request

Limitations/Interferences:

None specified.

Frequently Asked Questions (FAQs):

Not applicable

Related Words/Test:

Fasting Insulin, Insulin, serum

 

Test:Insulin Ab (Free & Total)
Other Test Request Name:
  • Insulin - anti-antibodies
  • IAA
  • Human Insulin Antibody

 

Test Composition:

• Total Insulin

• Free Insulin

Intended Use:

Can appear in insulin-dependent diabetics being treated with insulin.

 

Methodology:

Radioimmunoassay

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:

No patient preparation necessary.

Collection/Sample Container:

Red or Gold Tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

3 mL Serum

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

28 days

•   Refrigerated Temperature (2-8°C):

28 days

•   Freezer Temperature (-20°C):

28 days

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:

• Hemolyzed specimen
• Markedly lipemic specimen
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used

Running Day:

Batch Running

Cut Off Time:

Friday 4:00 PM

TAT/Releasing of Results:

14 working days after cut-off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Not Applicable

Limitations/Interferences:

None specified.

Frequently Asked Questions (FAQs):

Not applicable

Related Words/Test:Not Applicable

 

Test:Insulin Growth Factor 1
Other Test Request Name:
  • IGF, IGF-1
  • Somatomedin, Somatomedin C
  • Sm-C
  • Sulfation factor

 

Test Composition:

Not Applicable

Intended Use:

• This test measures the amount of insulin-like growth factor – 1 in blood.
• It aids to identify growth hormone (GH) deficiency.
• It is used to diagnose problems linked to too much GH (acromegaly; pituitary tumors) or too little GH (nutritional deficiencies, hypopituitarism, GH deficiency).

Methodology:

Enzyme-linked Immunosorbent Assay (ELISA)

Laboratory Section:

Serology

Special Instructions/Patient Preparations:

No patient preparation necessary.

Collection/Sample Container:

Red or Gold tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

1-2mL Serum

Note: Separate serum immediately.

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

Not Specified

•   Refrigerated Temperature (2-8°C):

24 hours

•   Freezer Temperature (-20°C):
  • >24 hours to 1 year (frozen only once prior to assay)
Transport Temperature:

Transport specimen at 2 – 10 °C (with cold packs)

Rejection Criteria:

 

• Lipemic specimens
• Hemolyzed specimens

• Icteric specimens 

• Samples containing sodium azide  
• Specimens showing bacterial contamination
• Improperly labeled specimens
• Quantity not sufficient
• Specimen stored exceeded outside required stability

Running Day:

Monday (Batch running)

Cut Off Time:

Sunday, 5:00 PM
(Day before running day)

TAT/Releasing of Results:

Tuesday, 9:00AM

Reference Interval/Result Interpretation:

Age (Years)

Normal Values (ng/mL)

Newborn

65.00 – 172.00

1

45.00 – 115.00

2

67.00 – 120.00

3

103.00 – 204.00

4

71.00 – 195.00

5

130.00 – 232.00

6

80.00 – 231.00

7

102.00 – 236.00

8

156.00 – 249.00

9

61.00 – 270.00

10

132.00 – 273.00

11

139.00 – 308.00

12

174.00 –372.00

13

203.00 – 411.00

14

153.00 – 416.00

15

119.00 – 375.00

16

106.00 – 379.00

17

175.00 – 349.00

18

186.00 – 338.00

19

168.00 – 387.00

20

246.00 – 363.00

21-25

97.00 – 304.00

26-30

137.00 – 278.00

31-35

120.00 – 229.00

36-40

190.00 – 294.00

41-45

107.00 – 216.00

46-50

86.00 – 196.00

51-55

102.00 – 212.00

56-60

96.00 – 217.00

61-65

81.00 – 216.00

66-70

80.00 – 176.00

71-75

57.00 – 199.00 

76-80

74.00 – 192.00

Reference: Demeditec IGF-I 600 ELISA DE4140

Version 10 - 06/18 JS (Updated 180628)

Limitations/Interferences:

• Bacterial contamination or heat inactivation of the specimens may affect the test results.
• Extremely high HAMA (human anti-mouse antibodies) concentrations may occasionally influence results.

Frequently Asked Questions (FAQs):

Not Applicable

Related Words/Test:

Growth Hormone, Somatomedin C

 

Test:Insulin Growth Factor BP3
Other Test Request Name:

• IgFBP3

• Insulin-Like Growth Factor Binding Protein

Test Composition:

Not applicable

Intended Use:

• IGFBP-3 is growth hormone (GH) responsive. Thus, levels are high in acromegaly and low in hypopituitarism, and levels increase in GH-deficient children after GH administration. Other causes of short stature that result in reduced IGFBP-3 levels include poorly controlled diabetes.

• The IGFBP-3 assay is useful in assessing nutritional status, since IGFBP-3 decreases during both caloric and protein restriction.

Methodology:

Immunoassay (IA)

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:

No patient preparation necessary.

Collection/Sample Container:

Red or Gold Tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

2 mL Serum

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

7 Days

•   Refrigerated Temperature (2-8°C):

14 Days

•   Freezer Temperature (-20°C):

1 Year

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:

• Hemolyzed specimen
• Markedly lipemic specimen
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used

Running Day:

Batch Running

Cut Off Time:

Monday, 12:00 PM

 

TAT/Releasing of Results:

2 weeks after cut-off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Applicable upon request.

Limitations/Interferences:

None specified.

Frequently Asked Questions (FAQs):

Not applicable

Related Words/Test:

Growth Hormone, Somatomedin C, IGF1

 

Test:Interleukin 1 Beta (EIA)
Other Test Request Name:IL-1
Test Composition:Not applicable
Intended Use:Interleukin 1-beta (IL-1 beta) is a paracrine cytokine. High levels are observed in septic shock. This cytokine is involved in the pathogenesis of rheumatoid arthritis and, in patients with this condition, its concentration in synovial fluid is significantly increased but not its serum concentration.
Methodology:Enzyme Immunoassay (EIA)
Laboratory Section:Special Test
Special Instructions/Patient Preparations:No patient preparation necessary.
Collection/Sample Container:EDTA or Violet Tube
Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:4 mL Plasma
Alternative Specimen and Volume Requirement:Not Applicable
Specimen Stability  
•   Room Temperature (15-25°C):24 Hours
•   Refrigerated Temperature (2-8°C):48 Hours
•   Freezer Temperature (-20°C):21 Days
Transport Temperature:Transport specimen at 2 – 8 °C (with cold packs)
Rejection Criteria:• Hemolyzed specimen
• Markedly lipemic specimen
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used
• Clotted specimen
• Over-filled or Under-filled tube
Running Day:Batch Running
Cut Off Time:Friday 4:00 PM
TAT/Releasing of Results:1 month after cut-off (excluding Saturdays, Sundays and Holidays)
Reference Interval/Result Interpretation:Available upon request
Limitations/Interferences:None specified.
Frequently Asked Questions (FAQs):Not applicable
Related Words/Test:Leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factor, cytokine protein