Laboratory Test Preparation

Test:Methaqualone (Quanti)
Other Test Request Name:Not applicable
Test Composition:Not applicable
Intended Use:Methaqualone is a central nervous system depressant, with similar effects to barbiturates, and has been prescribed as an anti-anxiety medication or sleep aid. The detection of methaqualone in serum or urine is useful in the detection of abuse by individuals.
Methodology:Gas Phase Chromatography / Mass Spectrometry / GC-MS)
Laboratory Section:Special Test
Special Instructions/Patient Preparations:No patient preparation necessary.
Collection/Sample Container:Red tube (DO NOT USE GOLD TUBE or any other type of tube with gel separator)
Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:3 mL Serum
Alternative Specimen and Volume Requirement:Not Applicable
Specimen Stability  
•   Room Temperature (15-25°C):72 Hours
•   Refrigerated Temperature (2-8°C):14 Days
•   Freezer Temperature (-20°C):30 Days
Transport Temperature:Transport specimen at 2 – 8 °C (with cold packs)
Rejection Criteria:• Hemolyzed specimen
• Markedly lipemic specimen
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used
Running Day:Batch Running
Cut Off Time:Friday 4:00 PM
TAT/Releasing of Results:4 weeks after cut-off (excluding Saturdays, Sundays and Holidays)
Reference Interval/Result Interpretation:Available upon request
Limitations/Interferences:None specified.
Frequently Asked Questions (FAQs):Not Applicable
Related Words/Test:Sedative and hypnotic medication

 

Test:Methotrexate MTX (EIA)
Other Test Request Name:• MTX
• Novatrex
Test Composition:Not applicable
Intended Use:Methotrexate is used to treat certain types of cancer of the breast, skin, head and neck, or lung. It is also used to treat severe psoriasis and rheumatoid arthritis. Do not use methotrexate to treat psoriasis or rheumatoid arthritis if you have liver disease (especially if caused by alcoholism), or a blood cell or bone marrow disorder.
Methodology:Enzyme Immunoassay (EIA)
Laboratory Section:Special Test
Special Instructions/Patient Preparations:No patient preparation necessary.
Collection/Sample Container:Red tube (DO NOT USE GOLD TUBE or any other type of tube with gel separator)
Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:3 mL Serum
Alternative Specimen and Volume Requirement:Not Applicable
Specimen Stability  
•   Room Temperature (15-25°C):72 Hours
•   Refrigerated Temperature (2-8°C):14 Days
•   Freezer Temperature (-20°C):30 Days
Transport Temperature:Transport specimen at 2 – 8 °C (with cold packs)
Rejection Criteria:• Hemolyzed specimen
• Markedly lipemic specimen
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used
Running Day:Batch Running
Cut Off Time:Friday 4:00 PM
TAT/Releasing of Results:4 weeks after cut-off (excluding Saturdays, Sundays and Holidays)
Reference Interval/Result Interpretation:Available upon request
Limitations/Interferences:None specified.
Frequently Asked Questions (FAQs):Not Applicable
Related Words/Test:Amethopterin, chemotherapy agent and immune system suppressant

 

Test:Methyl Hippuric Acid HPLC
Other Test Request Name:Not Applicable
Test Composition:

Not applicable

Intended Use:

A good marker for exposure to these compounds (occupational health). Complementary to or a replacement for assaying xylenes in the blood.

Methodology:

High Performance Liquid Chromatography

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:

Collection Procedures :
1. Instruct the patient to void at the beginning of collection period and discard the specimen.
2. Collect all urine including the final specimen voided at the end of collection period.
3. Label the bottle with patient name, date & time collection.
4. Submit the specimen to HPD
 

Collection/Sample Container:

Plastic leak proof clean container without preservative

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

100 mL Urine

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

8 Hours

•   Refrigerated Temperature (2-8°C):

7 Days

•   Freezer Temperature (-20°C):

28 Days

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:

•Quantity not sufficient
• Received room temperature
•Improperly labeled specimen
• Improper urine collection

Running Day:

Batch Running

Cut Off Time:

Friday 4:00 PM

TAT/Releasing of Results:

2 weeks after cut-off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Available upon request

Limitations/Interferences:

None specified.

Frequently Asked Questions (FAQs):

Not Applicable

Related Words/Test:

Not Applicable

 

Test:Methyl Malonic Acid
Other Test Request Name:

MMA

Test Composition:

Not applicable

Intended Use:

Methylmalonic acid is an intermediate in the conversion of propionic acid into succinic acid for use in the Krebs cycle.Urine levels are increased in pernicious anemia and methylmalonicacidemia which is characterized by neurological distress without convulsions (manifesting either immediately following birth or later), acidosis, ketosis and sometimes hyperlactacidemia with hyperammonemia and hyperglycinemia.

Methodology:

Gas Phase Chromatography / Mass Spectrometry (GC-MS)

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:

No patient preparation necessary.

Collection/Sample Container:

Leak proof clean container without preservative

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

100 mL of Random urine

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

48 Hours

•   Refrigerated Temperature (2-8°C):

7 Days

•   Freezer Temperature (-20°C):

28 Days

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:
  • Quantity not sufficient
  • Received room temperature
  • Improperly labeled specimen
  • Improper urine collection
Running Day:

Batch Running

Cut Off Time:

Friday, 4:00 PM

TAT/Releasing of Results:

1 month after cut-off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Available upon request

Limitations/Interferences:

None specified.

Frequently Asked Questions (FAQs):

Not Applicable

Related Words/Test:

Conjugate base methylmalonate

 

Test:MICRAL
Other Test Request Name:Urine Microalbumin Test
Test Composition:Not applicable
Intended Use:• For the routine detection of low concentration of Albumin in the urine.
• Use for screening, monitoring and detection of deteriorationg renal function in diabetic patients.
Methodology:Immunochemical Assay
Laboratory Section:Clinical Microscopy
Special Instructions/Patient Preparations:No patient preparation necessary.
Collection/Sample Container:Clean, leak-proof container
Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:5-10 mL Random urine

NOTE: For pediatric and geriatric patients, minimun of 3 mL is acceptable.
Alternative Specimen and Volume Requirement:Not applicable
Specimen Stability  
•   Room Temperature (15-25°C):2 Hours
•   Refrigerated Temperature (2-8°C):3 Days
•   Freezer Temperature (-20°C):Not Applicable
Transport Temperature:Transport specimen at 2 – 8 °C (with cold packs)
Rejection Criteria:• Quantity not sufficient (QNS): Samples less than 5 mL
NOTE: For pediatric and geriatric patients, minimun of 3 mL is acceptable.
• Urine which have been allowed to stand unrefrigerated for more than 3 days and show signs of bacterial decomposition (pH>8) should not be used.
• Specimen with fecal contamination.
Running Day:Daily
Cut Off Time:Monday to Saturday
• 6:00 PM

Sunday
• 3:00 PM
TAT/Releasing of Results:ROUTINE
• 3 hours after receipt of specimen/ arrival of messenger
STAT
• After 1½ hours from receipt of specimen/ arrival of messenger
Reference Interval/Result Interpretation:<20.00 mg/L
Limitations/Interferences:• A very low or very high fluid intake can lead to false positive or false negative results.
• False negative results can be produced by residual quantities or strongly oxidizing cleaning agents in the urine collection vessel.
• Falsely elevated result maybe caused by contamination of the specimen with pus, menstrual blood or vaginal discharge.
Frequently Asked Questions (FAQs):Not applicable
Related Words/Test:Albumin, Creatinine, Glucose, HbA1c, Urine Protein, Albumin/Creatinine Ratio

 

Test:Microalbumin/Creat. Ratio
Other Test Request Name:

• ACR
• UACR
• Albumin to Creatinine Ratio
• Microalbumin/Creatinine Ratio
• Microalbumin to Creatinine Ratio

Test Composition:

1. Random Urine Albumin
2. Random Urine Creatinine
3. Albumin/ Creatinine Ratio

Intended Use:

• Testing for microalbuminuria (low concentrations of albumin in urine) is recommended in patients with insulin-dependent diabetes mellitus (IDDM) as well as patients with non-insulin-dependent diabetes mellitus (NIDDM).
• This assay is intended for use in both screening and monitoring treatment of microalbuminuria.

Methodology:

Urine Protein: Immunoturbidimetric
Urine Creatinine: Enzymatic

Laboratory Section:

Chemistry

Special Instructions/Patient Preparations:

1. Refrain from eating a high meat diet and doing strenuous physical activity 8-12 hours before sample collection.
2. Refrain from taking medicines such as Acetaminophen (Paracetamol), N-acetyl-p-benzoquinone imine (NAPQI) metabolites, N-Acteylcysteine (NAC), Metamizole (Novaminsulfone, Dipyrone), 4-aminoantipyrine (4-AAP) and 4-Methylamino-antipyrine (4-MAP) metabolites 8-12 hrs before sample collection.

Note: If recommended preparation is not followed, you may accept but NOTE in clinical info.

Collection/Sample Container:

Clean plastic container

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

10-60 mL Random Urine

Alternative Specimen and Volume Requirement:

Not applicable

Specimen Stability  
•   Room Temperature (15-25°C):

2 Days

•   Refrigerated Temperature (2-8°C):

6 Days

•   Freezer Temperature (-20°C):

6 Months

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:

• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimens
• Improper urine collection

Running Day:

Daily

Cut Off Time:

Monday to Saturday: 10:00 PM

Sunday: 6:00PM

TAT/Releasing of Results:

RUNNING TIME: 6:00 AM onwards

RELEASING TIME:
ROUTINE
• 4 hours after receipt of specimen/ arrival of messenger
RELEASING TIME: STAT
• After 2½ hours from extraction/messenger arrival

Reference Interval/Result Interpretation:

Random Urine Creatinine:
MALE: 3.54-24.60 mmol/L (40.00-277.98 mg/dL)
FEMALE: 2.55-20.00 mmol/L (28.82-226.00 mg/dL)

Random Urine Protein:
<37.00 mg/L (<3.70 mg/dL)

Albumin/Creatinine Ratio:
MALE: <2.50 mg/mmol (<22.12 mg/g)
FEMALE: <3.50 mg/mmol (<30.97 mg/g)

Limitations/Interferences:

• Faslely elevated urine albumin results may be caused by contamination of the specimen with pus, menstrual blood, or vaginal discharge.
• Calcium dobesilate (e.g. Dexium), Levodopa and alpha?methyldopa cause artificially low creatinine results. Dicynone (Etamsylate) at therapeutic concentrations may lead to falsely low results.
• High homogentisic acid concentrations in urine samples lead to false results.
• Acetaminophen, Acetylcysteine and Metamizole are metabolized quickly. Therefore, interference from these substances is unlikely but cannot be excluded.

Frequently Asked Questions (FAQs):

Not applicable

Related Words/Test:

Albumin, Creatinine, Glucose, A1c, Urine Protein, Beta-2 Microglobulin

 

Test:Min.Residual DiseasePanel (BY APPOINTMENT PER PATIENT)
Other Test Request Name:

Minimal Residual Disease Panel

Test Composition:

Not Applicable

Intended Use:

Used to monitor Minimal residual disease (MRD). MRD is the name given to small numbers of leukaemic cells (cancer cells from the bone marrow) that remain in the patient during treatment, or after treatment when the patient is in remission (no symptoms or signs of disease). It is the major cause of relapse in cancer and leukemia.

Methodology:

Flow Cytometry

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:
  • No patient preparation necessary.
  • Request container from Special Test Section 
  • Call Special Test Section (By Appointment/Schedule.
  • Provide Clinical Abstract/Diagnosis

NOTE: Do not collect specimen without approval of Special Test Section

 

Collection/Sample Container:

Sodim Heparin (Green Top) for BONE MARROW ASPIRATE; or

Edta Tube (Violet top) for PERIPHERAL BLOOD

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

2 pcs of 4 mL Sodium Heparin (provided by Special Test); or

2pcs of 4ml EDTA Tube

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

24 Hours

•   Refrigerated Temperature (2-8°C):

Not Applicable

•   Freezer Temperature (-20°C):

Not Applicable

Transport Temperature:

Transport specimen at 15°C ~25 °C (room temperature)
NOTE: Send sample immediately to HPD Main Lab after extraction.

Rejection Criteria:
  • Exceeded sample stability requirement
  • Quantity not sufficient
  • Improperly labeled specimen
  • Improper collection tube used
  • Clotted Sample
  • Over-filled or Under-filled tube
Running Day:

Monday to Friday

Cut Off Time:

Monday to Friday, 7:00 AM

TAT/Releasing of Results:

7 days after cut-off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Not Applicable

Limitations/Interferences:

None Specified.

Frequently Asked Questions (FAQs):

Not Applicable

Related Words/Test:

CBCPLT, PBS

 

Test:Monospot Test/Heterophil
Other Test Request Name:

Not applicable

Test Composition:

Not applicable

Intended Use:

Helps to detect infectious Mononucleosis

Methodology:

Agglutination

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:

No patient preparation necessary.

Collection/Sample Container:

Red or Gold Tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

3 mL Serum

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

24 Hours

•   Refrigerated Temperature (2-8°C):

3 Days

•   Freezer Temperature (-20°C):

Not Applicable

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:
  • Hemolyzed specimen
  • Markedly lipemic specimen
  • Exceeded sample stability requirement
  • Quantity not sufficient
  • Improperly labeled specimen
  • Improper collection tube used
Running Day:

Monday to Friday

Cut Off Time:

Monday to Friday, 7:00 AM

TAT/Releasing of Results:

3 days after cut-off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Available upon request

Limitations/Interferences:

None specified

Frequently Asked Questions (FAQs):

Not applicable

Related Words/Test:

Mononucleosis Spot Test; Mononuclear Heterophile Test; Heterophile Antibody Test; Monospot, Heterophile Antibody Titer

 

Test:Morphine
Other Test Request Name:• M
• Dreamer
• Emma
• Emsel
• Pulbos
Test Composition:Not applicable
Intended Use:Used as a screening test for qualitative detection of morphine in urine.
Methodology:Lateral Flow Immunochromatography (Competitive Binding)
Laboratory Section:Drug Test
Special Instructions/Patient Preparations:No patient preparation necessary
Collection/Sample Container:60-mL clean, screw cap, polyethylene container
Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:60 mL urine
Alternative Specimen and Volume Requirement:Not applicable
Specimen Stability  
•   Room Temperature (15-25°C):Not specified
•   Refrigerated Temperature (2-8°C):48 Hours
•   Freezer Temperature (-20°C):Not specified
Transport Temperature:Not applicable
Rejection Criteria:• Incompatibility of the ID number on the specimen received by the laboratory with the number on the CCF.
• Absence of ID number on the specimen
• No printed Authorized Specimen Collector’s name and signature on the CCF.
• Broken or tampered seal on the specimen container
• Insufficient quantity of specimen, below 60 mL for single collection; below 30 mL for spilt collection
Running Day:Daily
Cut Off Time:Monday to Saturday
• 6:00 PM

Sunday
• 3:00 PM
TAT/Releasing of Results:ROUTINE
• 3 hours after receipt of specimen/ arrival of messenger
STAT
• After 1½ hours from receipt of specimen/ arrival of messenger
Reference Interval/Result Interpretation:Not applicable
Limitations/Interferences:• Other drugs with the same structure with the abused drugs
• Cross-reactant chemicals
• Bleach or alum
Frequently Asked Questions (FAQs):Not applicable
Related Words/Test:Pain medication

 

Test:Morphine and Derivatives
Other Test Request Name:Not applicable
Test Composition:1. Morphine
2. Codeine
3. Opiates
4. Heroine
Intended Use:Used as a screening test for qualitative detection of morphine, codeine and heroin in urine.
Methodology:Lateral Flow Immunochromatography (Competitive Binding)
Laboratory Section:Drug Test
Special Instructions/Patient Preparations:No patient preparation necessary
Collection/Sample Container:60-mL clean, screw cap, polyethylene container
Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:60 mL urine
Alternative Specimen and Volume Requirement:Not applicable
Specimen Stability  
•   Room Temperature (15-25°C):Not specified
•   Refrigerated Temperature (2-8°C):48 Hours
•   Freezer Temperature (-20°C):Not specified
Transport Temperature:Not applicable
Rejection Criteria:• Incompatibility of the ID number on the specimen received by the laboratory with the number on the CCF.
• Absence of ID number on the specimen
• No printed Authorized Specimen Collector’s name and signature on the CCF.
• Broken or tampered seal on the specimen container
• Insufficient quantity of specimen, below 60 mL for single collection; below 30 mL for spilt collection
Running Day:Daily
Cut Off Time:Monday to Saturday
• 6:00 PM

Sunday
• 3:00 PM
TAT/Releasing of Results:ROUTINE
• 3 hours after receipt of specimen/ arrival of messenger
STAT
• After 1½ hours from receipt of specimen/ arrival of messenger
Reference Interval/Result Interpretation:Not applicable
Limitations/Interferences:• Other drugs with the same structure with the abused drugs
• Cross-reactant chemicals
• Bleach or alum
Frequently Asked Questions (FAQs):Not applicable
Related Words/Test:Pain medication

 

Test:MSI (Electrophoresis/PCR)
Other Test Request Name:
  • Microsatellite Instability
  • Hereditary Nonpolyposis Colon Cancer / HNPCC
  • Lynch Syndrome
  • Mismatch Repair Gene Mutation
Test Composition:

Not Applicable

Intended Use:

Patients with early onset and family history of colon cancers are suggestive of Hereditary Nonpolyposis Colon Cancer (HNPCC) syndrome. Microsatellite instability analysis on tumor tissue is recommended as a screening test to identify individuals eligible for germline mutation analysis of mismatch repair genes.

 

Methodology:

Capillary Electrophoresis / Polymerase Chain Reaction (PCR)

 

 

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:

Need Histopathology report for the submitted tissue block

Collection/Sample Container:

Formalin-fixed, paraffin-embedded tissue collected in a paraffin block bag

 

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

1 pc of  Formalin-fixed, paraffin-embedded (FFPE) tissue block  with Pathologist report

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

5 Years

•   Refrigerated Temperature (2-8°C):

5 Years

•   Freezer Temperature (-20°C):

Not Applicable

Transport Temperature:

Transport specimen at 15°C ~25 °C (room temperature)

Rejection Criteria:
  • Exceeded sample stability requirement
  • Improperly labeled specimen
  • No histopathological report
Running Day:

Batch Running

Cut Off Time:

Monday to Friday, 12:00 PM

 

TAT/Releasing of Results:

2 weeks after cut-off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Not Applicable

Limitations/Interferences:

None Specified.

Frequently Asked Questions (FAQs):

Not Applicable

Related Words/Test:

CEA, Carcinoembryonic Antigen

 

Test:MTB GeneXpert(Others) (BY APPOINTMENT)
Other Test Request Name:

Not applicable

Test Composition:

Not applicable

Intended Use:

Used both to diagnose TB as well as to detect drug resistance. It can also detect the genetic mutations associatede with resistance to the drug rifampicin.

Methodology:

Polymerase Chain Reaction (PCR)

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:

NOTE: Do not collect specimen without approval of Special Test Section

  • Indicate SAMPLE TYPE AND SITE
  • TBPGE9 : use this code when request is MTB GeneXpert using other body fluid EXCEPT Sputum and Urine.
  • Note: Urine is not acceptable for TB GeneXpert testing
  • No patient preparation necessary
  • Call Special Test Section (By Appointment/Schedule).
Collection/Sample Container:

Sterile container

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:
  • At least 2 ml of other Body fluids EXCEPT Sputum and  Urine .
  • Note: CSF Sample should not be Xanthocromic
Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

Not Applicable

•   Refrigerated Temperature (2-8°C):

5 Days

•   Freezer Temperature (-20°C):

Not applicable

Transport Temperature:

Transport specimen at 2°C - 8°C (with cold packs)

Rejection Criteria:
  • Quantity Not sufficient
  • Improperly labeled specimen
  • Specimen stored and transported outside the required temperature
  • • Blood and Urine and Sputum specimen

 

Running Day:

Monday to Friday

Cut Off Time:

Monday to Friday, 7:00 AM

TAT/Releasing of Results:

1 week after cut-off  (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Not Applicable

Limitations/Interferences:

None specified.

Frequently Asked Questions (FAQs):

Not applicable

Related Words/Test:

Mycobacterium tuberculosis

 

Test:MTB/RIF GeneXpert (Sputum)
Other Test Request Name:
  • Mycobacterium tuberculosis PCR / Rifampicin-resistance 
  • M. tuberculosis-Drug Resistance (GeneXpert)
Test Composition:

Not applicable

Intended Use:

The MTB GeneXpert Assay is used for the detection of Mycobacterium tuberculosis and Rifampicin-resistance. It is intended to be used with specimens from untreated patients for whom there is clinical suspicion of tuberculosis (TB). and who have received no anti-tuberculosis therapy, or less than 3 days of therapy in the last 6 months.

Methodology:

Real Time Polymerase Chain Reaction (PCR)

Laboratory Section:

Molecular Diagnostics

Special Instructions/Patient Preparations:

Sputum collection:

  • Rinse mouth twice with water before collecting sputum sample.
  • Inhale deeply, cough vigorously, and expectorate the material into the container. Avoid spills or soiling the outside of the container.
  • Secure the lid on the collection container and then submit the container to a HPD Staff for checking of sample.
Collection/Sample Container:

Sterile container

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

At least 1ml of expectorated Sputum

Alternative Specimen and Volume Requirement:

1 mL Endotracheal Aspirate (ETA)

  • ETA samples from tubes must be transferred to a sterile screw cap container.
  • ETA samples should not be contaminated with blood, lubricants, foods & medicine.

 

Specimen Stability  
•   Room Temperature (15-25°C):

3 days

•   Refrigerated Temperature (2-8°C):

10 days

•   Freezer Temperature (-20°C):

Not acceptable

Transport Temperature:

Transport specimen at refrigerated temperature (2°C – 8 °C)

Rejection Criteria:
  • Bloody sputum sample
  • Sample with food particles
  • Exceeded sample stability/storage requirement
  • Quantity not sufficient
  • Improperly labeled specimen
Running Day:

Monday to Saturday

Cut Off Time:

5:00 PM

Note: Samples received after cut off time will be tested the following working day

TAT/Releasing of Results:

1 day excluding Sunday

Reference Interval/Result Interpretation:

MTB DETECTED, RIF DETECTED: indicates the sample is positive for both MTB and rifampicin resistance.

MTB DETECTED, RIF NOT DETECTED: indicates the sample is positive for MTB but not for rifampicin resistance.

MTB NOT DETECTED: indicates the sample is negative for MTB.

Limitations/Interferences:

None specified

Frequently Asked Questions (FAQs):

Q:What is the Xpert MTB/RIF Assay?

A: The Xpert MTB/RIF assay is a new test that is revolutionizing tuberculosis (TB) control by contributing to the rapid diagnosis of TB disease and drug resistance. The test simultaneously detects Mycobacterium tuberculosis complex (MTBC) and resistance to rifampin (RIF).

Q:  What is the public health significance of this test?

A:  Tuberculosis is one of the deadliest public health threats today, but there remains a lack of effective diagnostic tools. This contributes to the global TB problem, as untreated TB patients remain a source of infection for other members of the community. Untreated TB also results in considerable morbidity and mortality, especially in HIV co-infected individuals.

Q:  If the Xpert reports Rifampicin resistance does this mean the patient hasMulti-drug-resistant tuberculosis(MDR-TB)?

A:  The Xpert does not test for isoniazid so Line Probe Assay (LPA) or Drug Susceptibility Testing (DST) is needed to confirm MDR-TB and perform other drug testing.

Related Words/Test:

MTB nucleic acid amplification test (NAAT), TB Culture and Sensitivity, AFB stain

 

Test:MTHR
Other Test Request Name:
  •  p.Ala222Val mutation
  • Mutation of the methylenetetrahydrofolate reductase gene
  • MTHFR gene - c.677C>T mutation
Test Composition:

Not applicable

Intended Use:

Methylene tetrahydrofolate reductase (MTHFR) is one of the enzymes involved in the metabolism of the sulfur-containing amino acid, homocysteine. A specific point mutation (C677T) in the MTHFR gene gives rise to a heat-sensitive enzyme with reduced activity. Patients with this deficiency present with homocysteinuria and high blood levels of this amino acid which, being atherogenic and thrombogenic, constitute a cardiovascular risk. Clinical investigations are still necessary to define the role of this MTHFR variant in thrombotic risk.

Methodology:

Real-time PCR

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:

No patient preparation necessary.

Collection/Sample Container:

EDTA or Violet Tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

2 pcs of 4 mL EDTA whole blood

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

8 Days

•   Refrigerated Temperature (2-8°C):

8 Days

•   Freezer Temperature (-20°C):

Not Applicable 

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:

• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used
• Clotted Sample
• Over-filled or Under-filled tube

Running Day:

Batch Running

Cut Off Time:

Friday 4:00 PM

TAT/Releasing of Results:

3 weeks after cut-off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Not Applicable

Limitations/Interferences:

None specified.

Frequently Asked Questions (FAQs):

Not Applicable

Related Words/Test:

Not Applicable

 

Test:Mumps IgG
Other Test Request Name:

 

Mumps Antibody IgG

Test Composition:

Not Applicable

Intended Use:

For the determination of IgG-Class antibodies to Mumps virus in human serum.

Methodology:

 

ENZYME LINKED IMMUNOSORBET ASSAY (ELISA)

Laboratory Section:

Serology

Special Instructions/Patient Preparations:

No patient preparation necessary.

Collection/Sample Container:

Red or Gold top tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

2mL Serum

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

Not Specified

•   Refrigerated Temperature (2-8°C):

4 days

•   Freezer Temperature (-20°C):

>4 days

Transport Temperature:

Transport specimen at 2 – 10 °C (with cold packs)

Rejection Criteria:
  • Markedly lipemic specimens
  • Hemolyzed specimens
  • Specimens showing bacterial contamination
  • Improperly labeled specimens
  • Quantity not sufficient
  • Specimen stored exceeded outside required stability
Running Day:

Monday (Batch running)

Cut Off Time:

Sunday  5:00 PM

TAT/Releasing of Results:

Monday, 6:00PM

Reference Interval/Result Interpretation:

NEGATIVE: <0.80 Index

EQUIVOCAL: between 0.80 and 1.20 Index

POSITIVE: >1.2 Index

Limitations/Interferences:

 

Para-influenza viruses can interfere in the test

Frequently Asked Questions (FAQs):

Not Applicable

Related Words/Test:

Parotitis; Measles Immunity Test; Mumps Immunity Test; Measles Blood Test; Mumps Blood Test, Measles Antibody, IgM and IgG; Measles Viral Culture; Measles by RT-PCR; Mumps Antibody, IgM and IgG; Mumps Viral Culture; Mumps by RT-PCR