Test:NSE (Lung CA)
Other Test Request Name:

Neuron Specific Enolase

 

Test Composition:

Not applicable

Intended Use:

May be useful in treatment monitoring of small cell lung ca; also in brain injury like stroke, trauma epilepsy.

Methodology:

Electrochemiluminescence

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:
  • No patient preparation necessary.
  • Centrifuge and settle within one hour following sampling

NOTE:interference possible in patients treated with biotin (vitamin B7, B8 or H) or taking any food supplement containing biotin. Essential to STOP treatment 8 days before taking the sample.

 

Collection/Sample Container:

Red or Gold Tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:

3 mL Serum

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

1 Hour

•   Refrigerated Temperature (2-8°C):

7 Days

•   Freezer Temperature (-20°C):

28 Days

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:
  • Hemolyzed specimen
  • Markedly lipemic specimen
  • Exceeded sample stability requirement
  • Quantity not sufficient
  • Improperly labeled specimen
  • Improper collection tube used
Running Day:

Batch Running

Cut Off Time:

Friday, 4:00 PM

TAT/Releasing of Results:

2 weeks after cut-off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Available upon request

Limitations/Interferences:

None specified.

Frequently Asked Questions (FAQs):

Not applicable

Related Words/Test:

Glycolytic enzyme, small-cell lung cancer (SCLC)

 

Test:NST (Non-Stress Test)
Other Test Request Name:
  • Fetal Heart Rate Monitoring
  • Cardiotocography
Test Composition:

-

Intended Use:
The Non Stress Test (NST) is a prenatal test that measures fetal heart rate and response to movement in the third trimester to ensure that the baby is doing well and has no problem in receiving enough oxygen because of placental or umbilical cord problems.
 
During a non-stress test a baby's heart rate is monitored to see how it responds to the baby's movement. It is generally ordered when…
  • The baby is measuring small for his gestational age or is not very active.
  • The baby has decreased fetal movements or possible fetal growth problems.
  • There is any reason to suspect the placenta is not functioning adequately.
  • There is a condition in pregnancy characterized by a deficiency of amniotic fluid (oligohydramnios).
  • There is a multiple pregnancy with certain complications and/or a history of complications in a previous pregnancy.
  • A mother has an underlying medical condition, such as type 1 diabetes, heart disease or high blood pressure during pregnancy.
  • A pregnancy that has extended two weeks past due date (post-term pregnancy).
Methodology:

-

Laboratory Section:

IMAGING DEPARTMENT

Special Instructions/Patient Preparations:
The best time to do the procedure isafter 28 weeks (during the third trimester) of pregnancy. 
 
SCHEDULE
  • By Appointment - HPD Del Monte MomMe Clinic and HPD Las Piñas MomMe Clinic
Collection/Sample Container:

-

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:

-

Alternative Specimen and Volume Requirement:

-

Specimen Stability 

-

•   Room Temperature (15-25°C):

-

•   Refrigerated Temperature (2-8°C):

-

•   Freezer Temperature (-20°C):

-

Transport Temperature:

-

Rejection Criteria:

-

Running Day:

-

Cut Off Time:

-

TAT/Releasing of Results:

1 to 2 working days

Reference Interval/Result Interpretation:

-

Limitations/Interferences:

-

Frequently Asked Questions (FAQs):
How is NST being done?
  • The test involves attaching one belt to the mother’s abdomen to measure fetal heart rate and another belt to measure contractions. Movement, heart rate and “reactivity” of heart rate to movement is measured for 20-30 minutes. 
How often the test is done during pregnancy?
  • It depends on the situation. If the mother have a high risk pregnancy, women might start getting NST weekly or twice weekly after 28 weeks. Some may only need one isolated NST if the baby is not moving well. It is still best to consult your OB doctor.
What are the associated risks with the Non-Stress Test?
  • The test poses NO risk to the mother or the baby. The term nonstress means that nothing is done to place stress on the fetus during the procedure.
What to know about the test results?
  • A reactive non-stress result indicates that blood flow (and oxygen) to the fetus is adequate. A nonreactive non-stress result requires additional testing to determine whether the result is truly due to poor oxygenation, or whether there are other reasons for fetal non-reactivity (i.e. sleep patterns, certain maternal prescription or nonprescription drugs).
Related Words/Test:

-

 

Test:NTproBNP
Other Test Request Name:
  • BNP
  • N-terminal pro B-type natriuretic peptide
Test Composition:

Not applicable

Intended Use:
  • For prognostic information in patients with heart failure, coronary artery disease and valvular heart disease. Its particular strength is to rule out heart failure in patients who present with shortness of breath
  • For diagnosis of individuals suspected of having congestive heart failure
  • For detection of mild forms of cardiac dysfunction
  • For the risk stratification of patients with acute coronary syndrome and congestive heart failure
  • For monitoring the treatment in patients with left ventricular dysfunction
Methodology:

Electrochemiluminescence Immunoassay (ECLIA)

Laboratory Section:

Immunology

Special Instructions/Patient Preparations:
  • Samples should not be taken from patients receiving therapy with high biotin doses (i.e. > 5 mg/day) until at least 8 hours following the last biotin administration.
  • No fasting preparation required.
Collection/Sample Container:

Red / Gold tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:

2-3  mL serum

Alternative Specimen and Volume Requirement:

2-3 mL plasma ( K2 edta, K3 Edta)

Specimen Stability  
•   Room Temperature (15-25°C):

3 days

•   Refrigerated Temperature (2-8°C):

6 days

•   Freezer Temperature (-20°C):

24 months

Transport Temperature:

Specimen should be stored and transported at 2 – 8 °C (with cold packs).

Rejection Criteria:
  • Hemolyzed specimen
  • Markedly lipemic sample
  • Exceeded sample stability requirement
  • Quantity not sufficient
  • Improperly labeled specimen
  • Improper collection tube used
Running Day:

Tuesday - Thursday - Saturday

Cut Off Time:

7 pm on running Day

 

TAT/Releasing of Results:

 4 hours upon receipt of sample/ arrival of messenger

Reference Interval/Result Interpretation:

AVAILABLE UPON REQUEST

Limitations/Interferences:
  • Samples should not be taken from patients receiving therapy with high biotin doses (i.e. > 5 mg/day) until at least 8 hours following the last biotin administration.
  • In rare cases, interference due to extremely high titers of antibodies to analyte?specific antibodies, streptavidin or ruthenium can occur.
Frequently Asked Questions (FAQs):

Not Applicable

Related Words/Test:

Congestive Heart Failure, Heart Disease, Angina, Heart Attack and Acute Coronary Syndrome

 

Test:NTx
Other Test Request Name:
  • Peptides from NTx collagen degradation
  • Cross link
  • Osteomark
  • N-telopeptides
  • Collagen N-telopeptides
  • NTx - N-telopeptides of type I collagen - urines
Test Composition:

Not applicable

Intended Use:

This species corresponds to the N-terminal telopeptide of Type I collagen and its level in the urine gives a measure of the rate of bone resorption.

Methodology:

Enzyme-immunoassay (EIA)

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:
  • Fasting 8hrs
  • The urinary creatinine will be tested systematically on the same sample (SFBC recommendations). The sampling method chosen must be retained in repeat prescriptions
Collection/Sample Container:

Plastic screw cup container (sterile)
 

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:

100 mL of Random  Urine
 

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

24 Hours

•   Refrigerated Temperature (2-8°C):

5 Days

•   Freezer Temperature (-20°C):

28 Days

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:
  • Quantity not sufficient
  • Incorrect collection container used
  • Improperly labeled specimen
  • Exceed sample stability requirement
  • Urine specimens with preservatives
Running Day:

Batch Running

Cut Off Time:

Friday, 12:00 PM

TAT/Releasing of Results:

4 weeks after cut-off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Available upon request

Limitations/Interferences:

None Specified.

Frequently Asked Questions (FAQs):

Not Applicable

Related Words/Test:

Not Applicable

 

Test:Occult Bld(FIT)Test w/Kit
Other Test Request Name:

• Hidden Blood Test
• Occult Blood Test

• iFOBT

 

Test Composition:

Not applicable

Intended Use:

A colorectal cancer screening test that detects if there is any hidden blood in stool. The presence of blood may be a sign of lower gastrointestinal disorders that should be treated

Methodology:

Fecal Immunochemical Test (Immunochromatographic Assay)

Laboratory Section:

Clinical Microscopy

Special Instructions/Patient Preparations:

1. Carefully read Instructions before beginning your specimen collection.
2. No special dietary or medications restrictions.
Note: Eating fruits and vegetables can increase test accuracy
3. Check “Kit contents” ensure that it has all the components.
4. Remove any cleaners or bluing agents in your toilet bowl or tank and flush the toilet twice before sample collection.
5. Do not perform this test if patient has:
a. Hemorrhoids that are bleeding
b. Blood in urine
c. Blood in toilet bowl
d. 3 days before, during or after the menstrual period
e. If there are any bleeding cuts or wounds in the hands
f. Toilet bowl water is saltwater or rusty.

Collection/Sample Container:

Insure FIT Collection kit

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:

Fecal swabs collected from 2 different surface of the stool  of one time  bowel movement. Instructions for use for sample collection and handling are included in the insureONE Collection Kit.

Alternative Specimen and Volume Requirement:

Not applicable

Specimen Stability  
•   Room Temperature (15-25°C):

14 Days

•   Refrigerated Temperature (2-8°C):

Not applicable

•   Freezer Temperature (-20°C):

Not applicable

Transport Temperature:

Transport specimens immediately to HPD Laboratory at room temperature (15-25°C) after the second collection.

NOTE:
- Test card must be protected from heat (above 37°C) and direct sunlight.
- Do not delay transport of samples.

Rejection Criteria:

• Expired test card.
• Damaged, dirty or tampered test kits
• Non submission of test card after 14 days of first sample collection
• Exceeded sample stability requirement
• Improperly labeled specimens

Running Day:

Daily

Cut Off Time:

Monday to Saturday
• 6:00 PM

Sunday
• 3:00 PM

TAT/Releasing of Results:

ROUTINE
• 4 hours after receipt of specimen/ arrival of messenger
STAT
• After 1½ hours from receipt of specimen/ arrival of messenger

Reference Interval/Result Interpretation:

NEGATIVE

Limitations/Interferences:

• Patient with hemorrhoids, bleeding cuts or wounds can cause false-positive result.
• Patient with menstruation 3 days before, during, and 3 days after may cause false positive result.
• Bluing agents/ cleaners may interfere with result

Frequently Asked Questions (FAQs):

Not applicable

Related Words/Test:

• Bleeding hemorrhoids
• Bleeding in the lower colon or rectum

 

Test:Occult Blood (Guiac)
Other Test Request Name:

• Hidden Blood Test
• Occult Blood Test
• Hema-Screen Test

Stool Occult Blood

•Fecal Occult Blood Test (FOBT)

Guiac Based  FOBT

•gFOBT

 

*Fecal Occult Blood Test (FOBT)

*Guiac -based FOBT

*Hemocult

 

Test Composition:

Not applicable

Intended Use:

Simple, aesthetic, inexpensive test designated for use in collection and preparation of stool specimen. The positive test may result from either upper gastrointestinal bleeding or lower gastrointestinal bleeding and further investigation for peptic ulcers or a malignancy.

Methodology:

Guiac Principle

Laboratory Section:

Clinical Microscopy

Special Instructions/Patient Preparations:

1. Do not collect samples during or until 3 days after menstrual period or while having bleeding hemorrhoids or blood in the urine.
2. Do not use rectal medicines and tonics 3 days before and during testing period.
3. Do not received barium enemas 3 days before or during testing
4. Do not CONSUME the following:
a. Aspirin or other NSAIDs, except acetaminophen for 7 days
b. Red meat (beef, lamb) including processed meats and liver for 3 days
c. raw fruits, vegetables (melons, radishes, turnips, raw broccoli, cauliflower, mushroom, apple, banana, black grapes, pears, citrus fruits, plums and horseradish) for 3 days
d. Vitamin C in excess of 250 mg/24 hrs (250mg/day) from supplements, citrus, fruits and juice for 3 days
e. Oral medications (such as aspirin, indomethacin, reserpine, phenylbutazone, corticosteroids, etc.) and heavy alcohol, consumption may cause irritation or bleeding of the gastrointestinal tract and should be discontinued for 3 days prior to and during test period.

Collection/Sample Container:

Clean, leak-proof container

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:

Pea sized or size of 25 centavo coin stool

Alternative Specimen and Volume Requirement:

Not applicable

Specimen Stability  
•   Room Temperature (15-25°C):

2 Hours

•   Refrigerated Temperature (2-8°C):

24 Hours

•   Freezer Temperature (-20°C):

Not applicable

Transport Temperature:
  • Transport specimens immediately to HPD Laboratory at room temperature (15-25°C)within 2 hours
  •  Transport within 24 hours at Ref temperature (2-8°C)


NOTE: Do not delay transport of samples.

Rejection Criteria:

• Stool sample that has not met the patient preparation criteria
• Quantity not sufficient (QNS);sample less than 30 mg in volume
• Stool contaminated with urine or other fluids
• Stool not delivered in the laboratory within 2 hours of collection @ Room Temp (15-25°C) and exceeds 24 hours @ Ref Temp (2-8°C)

 

 

 

Running Day:

Daily

Cut Off Time:

Monday to Saturday
• 6:00 PM

Sunday
• 3:00 PM

TAT/Releasing of Results:

ROUTINE
• 4 hours after receipt of specimen/ arrival of messenger
STAT
• After 1½ hours from receipt of specimen/ arrival of messenger

Reference Interval/Result Interpretation:

NEGATIVE

Limitations/Interferences:

• Ingestion of ascorbic acid (Vit C) in high doses has been, shown to cause false negative results.
• Peroxidase from fruits and vegetables can cause false positive or negative results.
• Diet rich in meats, green and leafy vegetables, poultry, and fish may produce false positive results.
• Oral medications (such as aspirin, indomethacin, reserpine, phenylbutazone, corticosteroids, etc.) and heavy alcohol consumption may cause irritation or bleeding of the gastrointestinal tract.
• Specimens will be positive if contaminated by menstrual or hemorrhoidal blood or povidone-iodine solution.
• Inadequate stool on the slide may produce false negative results.

Frequently Asked Questions (FAQs):

Not applicable

Related Words/Test:

•Gastrointestinal diseases.
• Screening for Colorectal cancer

 

Test:Oestrone
Other Test Request Name:• Estrone
• E1
Test Composition:Not applicable
Intended Use:Estrone is both secreted by the ovary and (about 30% in females) generated by the conversion of precursors like estradiol and delta-4 androstenedione. In menstruating women, estrone levels are lower than those of estradiol but this situation is reversed following menopause when estrone becomes the predominant estrogen. Since there is no more ovarian production, all of the estrone is derived from the peripheral metabolism of delta-4 androstenedione.
Methodology:Radio Immuno Assay (RIA)
Laboratory Section:Special Test
Special Instructions/Patient Preparations:No patient preparation necessary.
Collection/Sample Container:Red or Gold Tube
Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:3 mL Serum
Alternative Specimen and Volume Requirement:Not Applicable
Specimen Stability  
•   Room Temperature (15-25°C):4 Days
•   Refrigerated Temperature (2-8°C):7 Days
•   Freezer Temperature (-20°C):30 Days
Transport Temperature:Transport specimen at 2 – 8 °C (with cold packs)
Rejection Criteria:• Hemolyzed specimen
• Markedly lipemic specimen
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used
Running Day:Batch Running
Cut Off Time:Friday 4:00 PM
TAT/Releasing of Results:2 weeks after cut-off (excluding Saturdays, Sundays and Holidays)
Reference Interval/Result Interpretation:Available upon request
Limitations/Interferences:None specified.
Frequently Asked Questions (FAQs):Not applicable
Related Words/Test:Estrogenic hormone secreted by the ovary

 

Test:OGTT Non-Pregnant (75 gms)
Other Test Request Name:

• Oral Glucose Tolerance Test
• Glucose Tolerance Test
• GTT

Test Composition:

1. OGTT FBS
2. OGTT 2 Hours

Intended Use:

• Used to support or rule out a diagnosis of diabetes mellitus
• Can also be a part of a workup for unexplained hypertriglyceridemia, neuropathy, impotence, renal diseases, or retinopathy.
• This test may be ordered when there is sugar in the urine or when the fasting blood sugar level is significantly elevated.

Methodology:

Hexokinase

Laboratory Section:

Chemistry

Special Instructions/Patient Preparations:

• 8-12 hours fasting prior to testing.
• Eat generous amounts of carbohydrate on each of the three days before the test. The carbohydrate foods that are recommended are bread, cereal, potatoes, vegetables, and fruit.
• Take your usual amounts of milk, meat, fish, eggs, cheese, butter and margarine and, as well, eat the following foods daily during each of the three days before the test in at least these amounts:
- 3 slices of bread or toast
- 1 serving of breakfast cereal or porridge, spaghetti
- 1 medium potato or one serving of rice
- 3 servings of vegetables
- 3 servings of fruit (fresh, cooked, canned or juice)
- For morning tea and afternoon tea have 2 biscuits or an extra slice of bread or toast.
***NOTE: The 3 day diet is desirable but not essential. For any patient whose doctor indicates that the diet is not required, please follow the doctor’s instructions.
• Take any medications on the same schedule prescribed by your physician, such as blood pressure or heart medication. Do not take any over-the-counter medications for 12 hours before the test, such as cough or cold preparations, vitamins, aspirin, etc.
(The OGTT should be postponed if the patient becomes ill, even with common illnesses such as the flu or a severe cold.)

Note:
• Prolonged fasting is highly discouraged since various metabolic changes associated with starvation may begin to develop. Water intake is acceptable.
• Patient is required to consume the entire amount of the specially-formulated glucose solution within a 5-minute period.
• Note in clinical info if patient drank more than 4 glasses of water during fasting period, ask patient for medical history as this is rather unusual.
• Note in clinical info in case patient overfasts and insists on proceeding test.
• Samples without preservatives (NaF) must be separated from red cells within 30 mins of blood extraction.

Collection/Sample Container:

Red or Gold tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:

1-3 mL Serum

Alternative Specimen and Volume Requirement:

1-3mL Plasma (Li-heparin and K2-EDTA, NaF/Na2 EDTA, KF/Na2 EDTA, NaF/K?Oxalate and NaF/citrate/Na2?EDTA)

Specimen Stability  
•   Room Temperature (15-25°C):

Serum (no hemolysis): 8 hours
Plasma (fluoride): 3 days

•   Refrigerated Temperature (2-8°C):

72 Hours

•   Freezer Temperature (-20°C):

Not specified

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:

• Hemolyzed specimen
• Markedly lipemic specimen
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used

Running Day:

Daily

Cut Off Time:

Monday to Saturday: 10:00 PM

Sunday: 6:00PM

TAT/Releasing of Results:

RUNNING TIME: 6:00 AM onwards

RELEASING TIME: ROUTINE
• 4 hours after receipt of specimen/ arrival of messenger
RELEASING TIME: STAT
• After 2½ hours from extraction/messenger arrival

                              

Reference Interval/Result Interpretation:

• FBS: 3.89~5.49 mmol/L (70.10~98.93 mg/dL)
ADA Recommendations 2015:
Pre-diabetics: 7.8-11.0 mmol/L(140-199 mg/dL)
Diabetics: >/=11.10 mmol/L(>/=200.00 mg/dL)
• OGTT 2 Hours: <7.80 mmol/L (<140.56 mg/dL)
ADA Recommendations 2015:
Pre-Diabetics: 7.80-11.00 mmol/L(140.56-198.22 mg/dL)
Diabetics : >/= 11.10 mmol/L(>/= 200.02 mg/dL)

Limitations/Interferences:

• The OGTT is not indicated in these situations:
- Persistent fasting hyperglycemia >140 mg/dL or >7.8 mmol/L
- Persistent fasting normal plasma glucose
- Patients with overt diabetes mellitus
- Persistent 2-hour plasma glucose >200 mg/dL or >11.1 mmol/L
• OGTT is contraindicated in patients with a recent history of surgery, MI, or labor and delivery; these conditions can produce invalid values.
• Test has limited value in diagnosis of diabetes mellitus in children and is rarely indicated for that purpose.
• In very rare cases, gammopathy, in particular type IgM (Waldenstrom’s
macroglobulinemia), may cause unreliable results.

Frequently Asked Questions (FAQs):

Q: When should testing for diabetes start?
A: Testing is recommended for persons above 45 years old and asymptomatic adults of any age who are overweight or obese. Testing to detect type 2 diabetes should be considered in children and adolescents who are overweight or obese and who have two or more additional risk factors for diabetes.

Q: How often should testing be done?
A: The appropriate interval between tests is not known. Although if tests are normal, repeat testing carried out at a minimum of 3-year intervals is reasonable. The rationale for the 3-year interval is that with this interval, the number of false-positive tests that require confirmatory testingwill be reduced and individuals with false-negative tests will be retested before substantial time elapses and complications develop.

Related Words/Test:

Diabetes, DM

 

Test:OGTT-Pregnant (100 gms)
Other Test Request Name:

• Oral Glucose Tolerance Test
• Glucose Tolerance Test
• GTT

Test Composition:

1. OGTT FBS
2. OGTT 1 Hour
3. OGTT 2 Hours
4. OGTT 3 Hours

Intended Use:

• OGTT-Pregnant (100 gms) test is used to detect gestational diabetes.
• Second step to the Two-step strategy of screening for and diagnosis of Gestational Diabetes Mellitus (GDM).

Methodology:

Hexokinase

Laboratory Section:

Chemistry

Special Instructions/Patient Preparations:

• 8-12 hours fasting prior to testing.
• Eat generous amounts of carbohydrate on each of the three days before the test. The carbohydrate foods that are recommended are bread, cereal, potatoes, vegetables, and fruit.
• Take your usual amounts of milk, meat, fish, eggs, cheese, butter and margarine and, as well, eat the following foods daily during each of the three days before the test in at least these amounts:
- 3 slices of bread or toast
- 1 serving of breakfast cereal or porridge, spaghetti
- 1 medium potato or one serving of rice
- 3 servings of vegetables
- 3 servings of fruit (fresh, cooked, canned or juice)
- For morning tea and afternoon tea have 2 biscuits or an extra slice of bread or toast.
***NOTE: The 3 day diet is desirable but not essential. For any patient whose doctor indicates that the diet is not required, please follow the doctor’s instructions.
• Take any medications on the same schedule prescribed by your physician, such as blood pressure or heart medication. Do not take any over-the-counter medications for 12 hours before the test, such as cough or cold preparations, vitamins, aspirin, etc.
(The OGTT should be postponed if the patient becomes ill, even with common illnesses such as the flu or a severe cold.)

Note:
• Prolonged fasting is highly discouraged since various metabolic changes associated with starvation may begin to develop. Water intake is acceptable.
• Patient is required to consume the entire amount of the specially-formulated glucose solution within a 5-minute period.
• Note in clinical info if patient drank more than 4 glasses of water during fasting period, ask patient for medical history as this is rather unusual.
• Note in clinical info in case patient overfasts and insists on proceeding test.
• Samples without preservatives (NaF) must be separated from red cells within 30 mins of blood extraction.

Collection/Sample Container:

Red or Gold tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:

1-3 mL Serum

Alternative Specimen and Volume Requirement:

1-3mL Plasma (Li-heparin and K2-EDTA, NaF/Na2 EDTA, KF/Na2 EDTA, NaF/K?Oxalate and NaF/citrate/Na2?EDTA)

Specimen Stability  
•   Room Temperature (15-25°C):

Serum (no hemolysis): 8 hours
Plasma (fluoride): 3 days

•   Refrigerated Temperature (2-8°C):

72 Hours

•   Freezer Temperature (-20°C):

Not specified

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:

• Hemolyzed specimen
• Markedly lipemic specimen
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used

Running Day:

Daily

Cut Off Time:

Monday to Saturday: 10:00 PM

Sunday: 6:00PM

TAT/Releasing of Results:

RUNNING TIME: 6:00 AM onwards

RELEASING TIME: ROUTINE
• 4 hours after receipt of specimen/ arrival of messenger
RELEASING TIME: STAT
• After 2½ hours from extraction/messenger arrival

                              

Reference Interval/Result Interpretation:

• FBS: 3.89~5.49 mmol/L (70.10~98.93 mg/dL)
ADA Recommendations 2015:
Pre-diabetics: 7.8-11.0 mmol/L(140-199 mg/dL)
Diabetics: >/=11.10 mmol/L(>/=200.00 mg/dL)
• OGTT 1 Hour: <10.00 mmol/L (<180.20 mg/dL)
• OGTT 2 Hours: <8.60 mmol/L (<154.97 mg/dL)
ADA Recommendations 2015:
Pre-Diabetics: 7.80-11.00mmol/L(140.56-198.22 mg/dL)
Diabetics : >/= 11.10 mmol/L(>/= 200.02 mg/dL)
• OGTT 3 Hours: <7.80 mmol/L (<140.56 mg/dL)

Limitations/Interferences:

In very rare cases, gammopathy, in particular type IgM (Waldenstrom’s
macroglobulinemia), may cause unreliable results.

Frequently Asked Questions (FAQs):

Not applicable

Related Words/Test:

Diabetes, DM, Pregnancy, Gestational Diabetes

 

Test:OGTT-Pregnant (75 gms)
Other Test Request Name:

• Oral Glucose Tolerance Test
• Glucose Tolerance Test
• GTT

Test Composition:

1. OGTT FBS
2. OGTT 1 Hour
3. OGTT 2 Hours

Intended Use:

• OGTT-Pregnant (75 gms) test is used to detect gestational diabetes.
• One-step strategy of screening for and diagnosis of Gestational Diabetes Mellitus (GDM).

Methodology:

Hexokinase

Laboratory Section:

Chemistry

Special Instructions/Patient Preparations:

• 8-12 hours fasting prior to testing.
• Eat generous amounts of carbohydrate on each of the three days before the test. The carbohydrate foods that are recommended are bread, cereal, potatoes, vegetables, and fruit.
• Take your usual amounts of milk, meat, fish, eggs, cheese, butter and margarine and, as well, eat the following foods daily during each of the three days before the test in at least these amounts:
- 3 slices of bread or toast
- 1 serving of breakfast cereal or porridge, spaghetti
- 1 medium potato or one serving of rice
- 3 servings of vegetables
- 3 servings of fruit (fresh, cooked, canned or juice)
- For morning tea and afternoon tea have 2 biscuits or an extra slice of bread or toast.
***NOTE: The 3 day diet is desirable but not essential. For any patient whose doctor indicates that the diet is not required, please follow the doctor’s instructions.
• Take any medications on the same schedule prescribed by your physician, such as blood pressure or heart medication. Do not take any over-the-counter medications for 12 hours before the test, such as cough or cold preparations, vitamins, aspirin, etc.
(The OGTT should be postponed if the patient becomes ill, even with common illnesses such as the flu or a severe cold.)

Note:
• Prolonged fasting is highly discouraged since various metabolic changes associated with starvation may begin to develop. Water intake is acceptable.
• Patient is required to consume the entire amount of the specially-formulated glucose solution within a 5-minute period.
• Note in clinical info if patient drank more than 4 glasses of water during fasting period, ask patient for medical history as this is rather unusual.
• Note in clinical info in case patient overfasts and insists on proceeding test.
• Samples without preservatives (NaF) must be separated from red cells within 30 mins of blood extraction.

Collection/Sample Container:

Red or Gold tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:

1-3 mL Serum

Alternative Specimen and Volume Requirement:

1-3mL Plasma (Li-heparin and K2-EDTA, NaF/Na2 EDTA, KF/Na2 EDTA, NaF/K?Oxalate and NaF/citrate/Na2?EDTA)

Specimen Stability  
•   Room Temperature (15-25°C):

Serum (no hemolysis): 8 hours
Plasma (fluoride): 3 days

•   Refrigerated Temperature (2-8°C):

72 Hours

•   Freezer Temperature (-20°C):

Not specified

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:

• Hemolyzed specimen
• Markedly lipemic specimen
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used

Running Day:

Daily

Cut Off Time:

Monday to Saturday: 10:00 PM

Sunday: 6:00PM

TAT/Releasing of Results:

RUNNING TIME: 6:00 AM onwards

RELEASING TIME: ROUTINE
• 4 hours after receipt of specimen/ arrival of messenger
RELEASING TIME: STAT
• After 2½ hours from extraction/messenger arrival

                              

Reference Interval/Result Interpretation:

• FBS: 3.89~5.49 mmol/L (70.10~98.93 mg/dL)
ADA Recommendations 2015:
Pre-diabetics: 7.8-11.0 mmol/L(140-199 mg/dL)
Diabetics: >/=11.10 mmol/L(>/=200.00 mg/dL)
• OGTT 1 Hour: <10.00 mmol/L (<180.20 mg/dL)
• OGTT 2 Hours: <8.50 mmol/L (<153.17 mg/dL)
ADA Recommendations 2015:
Pre-Diabetics: 7.80-11.00 mmol/L (140.56-198.22 mg/dL)
Diabetics : >/= 11.10 mmol/L (>/= 200.02 mg/dL)

Limitations/Interferences:

In very rare cases, gammopathy, in particular type IgM (Waldenstrom’s macroglobulinemia), may cause unreliable results.

Frequently Asked Questions (FAQs):

Not applicable

Related Words/Test:

Diabetes, DM, Pregnancy, Gestational Diabetes

 

Test:Oligoclonal Bands
Other Test Request Name:
  • Cerebrospinal Fluid-Immunofixation
  • Oligloconal Panel
Test Composition:

1. Immunofixatin Electrophoresisis
2. CSF IgG

Intended Use:

Helps support the diagnosis of Multiple Sclerosis (MS), however it does not confirm the diagnosis.

Methodology:

Hydragel Electrophoresis

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:

No patient preparation necessary.

Collection/Sample Container:

Sterile, leak proof clean container

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:

2 mL CSF

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

Not Applicable

•   Refrigerated Temperature (2-8°C):

24 Hours

•   Freezer Temperature (-20°C):

14 Days

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:
  • Quantity not sufficient
  • Non-sterile specimen container
  • Improperly labeled specimen
Running Day:

Monday

Cut Off Time:

Tuesday to Friday, 7:00 A.M

TAT/Releasing of Results:

7 working Days after running day (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Available upon request

Limitations/Interferences:

None specified.

Frequently Asked Questions (FAQs):

Not applicable

Related Words/Test:

Immunoglobulins, Multiple Sclerosis

 

Test:Omega 3 and Fatty Acids
Other Test Request Name:

Not applicable

Test Composition:

Not Applicable

Intended Use:

The Omega-3 Index is a measure of Omega-3 blood levels. It is the measurement of Docosahexaenoic Acid (DHA) and Eicosapentaenoic Acid (EPA) Omega-3 fatty acids as a fraction of the total fatty acids in your red blood cells and have antiinflammatory properties. Too much inflammation or persistent inflammation at low levels is undesirable.

Methodology:

Gas Chromatography

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:
  • No patient preparation necessary.
  • Request Omega 3 Collection Kit to Special test section atleast 2 days prior to collection

Collection Instructions:

  1. Get the Sample Collection Card inside the silver resealable biohazard
  2. Fill out the information requested on the Sample Collection Card
  3. Wash hands thoroughly with warm water. Then hold your arm down at your side for 2 seconds to increase blood flow to the fingers.

Collect the sample:

  • Open the alcohol wipe and clean the side (not the pad or tip) of your finger you plan to use, typically the middle or ring finger. Wipe away the excess alcohol with the cotton ball.
  • Twist off the blue protective lancet cover.
  • Place the lancet device firmly on the side of your clean finger and quickly press downward into your finger.
  • This will release a small spring-loaded needle and you will feel a faint sting.
  • Allow the blood to collect on your finger until a drop forms.
  • If needed, lightly squeeze your finger to form a drop.
  • Before the blood drop naturally falls, lightly touch it to the center of the sample collection paper.
  • When finished, apply pressure to the finger with the cotton ball to stop bleeding. Apply adhesive bandage if you wish.
  • Let the blood spot dry for 15 – 20 minutes. Once the spot is dry, fold the Sample Collection Card flap so it covers the blood spot.
  • Immediately place the Sample Collection Card inside the silver resealble biohazard pack with the desiccant packet and seal.
  • Discard the lancet and other supplies in the trash.
Collection/Sample Container:

Omega 3 and Fatty Acids Kit will be provided by HPD Special Test Section.

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:

Dried Blood Spot

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

14 Days

•   Refrigerated Temperature (2-8°C):

Not Applicable

•   Freezer Temperature (-20°C):

Not Applicable

Transport Temperature:

Transport specimen at 15°C ~25 °C (room temperature)

Rejection Criteria:
  • DBS not completely filled.
  • Improperly labeled specimen
  • Incomplete data on Specimen Cillection Card
Running Day:

Batch Running

Cut Off Time:

Friday, 4:00 PM

TAT/Releasing of Results:

2 weeks after cut-off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Not Applicable

Limitations/Interferences:

None specified.

Frequently Asked Questions (FAQs):

 

Q:What is the Omega-6 to Omega-3 Ratio?

A:Omega-6’s are important fatty acids mainly responsible for the

inflammatory response. Inflammation is important in fightinginfection and disease.Omega-6 is found in vegetable oils, cheaper margarines (and oftenused in food manufacturing pastries, pre-cooked oven bakedpotato chips), grains, seeds and nuts and reducing intake fromthese foods can help reach an optimal ratio.

Lower Omega-6 to Omega-3 ratios (less than 3) have beenassociated with lower incidence of certain cancers,cardiovascular, autoimmune and inflammatory diseases.

Q: What is the Arachidonic Acid (AA) to Eicosapentaenoic Acid (EPA) Ratio?  

A:Arachidonic acid (AA) is an Omega-6 polyunsaturated fat that is produced from the intake of vegetable oils and red meat. It is proinflammatory and plays an important role in warding off infections. Eicosapentaenoic acid (EPA) is one of the Omega-3 fatty acids and has anti-inflammatory properties and counterbalances the pro-inflammatory effects of AA. The AA/EPA ratio has been used as an indicator of cellular inflammation. An AA/EPA ratio between 1.5 and 3.0 is desirable.

 

 

Related Words/Test:

Oocosahexaenoic acid (DHA) and Eicosapentaenoic acid (EPA)

 

Test:Opiates
Other Test Request Name:Not applicable
Test Composition:Not applicable
Intended Use:Used as a screening test for qualitative detection of opiates in urine.
Methodology:Lateral Flow Immunochromatography (Competitive Binding)
Laboratory Section:Drug Test
Special Instructions/Patient Preparations:No patient preparation necessary
Collection/Sample Container:60-mL clean, screw cap, polyethylene container
Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:60 mL urine
Alternative Specimen and Volume Requirement:Not applicable
Specimen Stability  
•   Room Temperature (15-25°C):Not specified
•   Refrigerated Temperature (2-8°C):48 Hours
•   Freezer Temperature (-20°C):Not specified
Transport Temperature:Not applicable
Rejection Criteria:• Incompatibility of the ID number on the specimen received by the laboratory with the number on the CCF.
• Absence of ID number on the specimen
• No printed Authorized Specimen Collector’s name and signature on the CCF.
• Broken or tampered seal on the specimen container
• Insufficient quantity of specimen, below 60 mL for single collection; below 30 mL for spilt collection
Running Day:Daily
Cut Off Time:Monday to Saturday
• 6:00 PM

Sunday
• 3:00 PM
TAT/Releasing of Results:ROUTINE
• 3 hours after receipt of specimen/ arrival of messenger
STAT
• After 1½ hours from receipt of specimen/ arrival of messenger
Reference Interval/Result Interpretation:Not applicable
Limitations/Interferences:• Other drugs with the same structure with the abused drugs
• Cross-reactant chemicals
• Bleach or alum
Frequently Asked Questions (FAQs):Not applicable
Related Words/Test:Papaver somniferum, morphine, codeine, and thebaine

 

Test:Organic Acid-Temporarily Unavailable (currently not accepting samples)
Other Test Request Name:

Urinary Organic Acid Profile

Test Composition:

Not applicable

Intended Use:

Organic acidurias are a group of inborn errors of metabolism that may lead to acute life-threatening illness, developmental delays, and metabolic decompensation. Positive results may warrant confirmation by alternative specific methods.

a well-established procedure for diagnosis of classical and other organic acidurias, ornithine transcarbamoylase deficienc, and fatty acid oxidation defects.

Methodology:

Gas Chromatography/Mass Spectrometry

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:
  • No patient preparation necessary.
  • Note date and time of sample collection
  • Note medication and diagnosis
  • Note feeding history 48Hrs before sample collection
  • Inform laboratory 1 day prior urine collection for schedule.
  • Need Requesting physician with License No.

For newborn and geriatic patients: SPLIT COLLECTION (within 12Hrs): DO NOT COLLECT SAMPLE THAT WILL FALL ON WEEKEND

  • Note date and time of sample collection (each container)
Collection/Sample Container:

Plastic leak proof clean container

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:
  • 60 mL Random Urine
  • For Newborn/Geriatic Patients: atleast 30mL (split collection) - store in freezer
Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

Not Applicable

•   Refrigerated Temperature (2-8°C):

1 Hour(for Single Collection Only)

•   Freezer Temperature (-20°C):

12 Hours(for Single and Split Collection)

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:
  • Quantity not sufficient
  • Received room temperature
  • Improperly labeled specimen
  • Improper urine collection
  • Incomplete details
Running Day:

Tuesday and Thursday

Cut Off Time:

Monday and Wednesday, 7:00 AM

TAT/Releasing of Results:

4 weeks after cut-off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Available upon request

Limitations/Interferences:

None specified.

Frequently Asked Questions (FAQs):

Not applicable

Related Words/Test:

Metabolic Analysis, gastrointestinal function, cellular and mitochondrial energy production, neurotransmitter processing, and amino acid/organic acid balance as influenced by vitamin/mineral cofactors

 

Test:Organic and Metabolic Scr-Temporarily Unavailable (currently not accepting samples)
Other Test Request Name:

Organic Acid and Metabolic Screening

Test Composition:

Not applicable

Intended Use:

 

 

Involves analysing urine to detect altered levels ofintermediate metabolites that result from incomplete metabolism of amino or organicacids. The analytical methods used to diagnose amino acid and organic acid disordersdiffer.

Methodology:

Gas Chromatography/Mass Spectrometry

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:
  • No patient preparation necessary.
  • Note date and time of sample collection
  • Note medication and diagnosis
  • Note feeding history 48Hrs before sample collection
  • Inform laboratory 1 day prior urine collection for schedule.
  • Need Requesting physician with License No.

For newborn and geriatic patients: SPLIT COLLECTION (within 12Hrs): DO NOT COLLECT SAMPLE THAT WILL FALL ON WEEKEND

Note date and time of sample collection (each container)

Collection/Sample Container:

Plastic leak proof clean container

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:
  • 60 mL Random Urine
  • For Newborn/Geriatic Patients: atleast 30mL (split collection) - store in freezer
Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

Not Applicable

•   Refrigerated Temperature (2-8°C):

1 Hour(for Single Collection Only)

 

•   Freezer Temperature (-20°C):

12 Hours(for Single and Split Collection)

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs) -for Single Collection

Transport specimen at -20 °C (with ice packs) -for Single and Split

Rejection Criteria:
  • Quantity not sufficient
  • Received room temperature
  • Improperly labeled specimen
  • Improper urine collection
Running Day:

Tuesday and Thursday

 

Cut Off Time:

Monday and Wednesday, 7:00 AM

TAT/Releasing of Results:

2 months after cut-off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Available upon request

Limitations/Interferences:

None specified.

Frequently Asked Questions (FAQs):

Not applicable

Related Words/Test:

Organic aciduria, metabolic disorders