Laboratory Test Preparation

• Panel Reactive Antibody Class 2

Not applicable

Luminometry

Special Test

• No patient preparation necessary.
• Pease get form to Special Test Section
• Fill up the DONOR and RECIPIENT FORM 
• Call Special Test Section (By Appointment/Schedule).

NOTE: Do not collect specimen without approval of Special Test Section

Red tube only

2 mL Serum

Not Applicable

Not applicable

Not applicable

5 days

Transport specimen with dry ice

• Hemolyzed specimen
• Markedly lipemic specimen
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used

Tuesday 7:00 AM

Monday 7AM

Friday 5:00 PM

Available upon request

None specified.

Not applicable

for post-transplant monitoring and consists of HLA Class I & II Panel Reactive Antibody (PRA), the presence/absence of DSA(s), and the strength/trend of the DSA(s).

• Panel Reactive Antibody Specific Class 1

Not applicable

Luminometry

Special Test

• No patient preparation necessary.
• Pease get form to Special Test Sectio
• Fill up the DONOR and RECIPIENT FORM 
• Call Special Test Section (By Appointment/Schedule).

NOTE: Do not collect specimen without approval of Special Test Section

Red tube only.

2 mL Serum

Not Applicable

Not applicable

Not applicable

5 days

Transport specimen with dry ice

• Hemolyzed specimen
• Markedly lipemic specimen
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used

Tuesday 7:00 AM

Monday 7:00 AM

Friday 5:00 PM

Available upon request

None specified.

Not applicable

for post-transplant monitoring and consists of HLA Class I & II Panel Reactive Antibody (PRA), the presence/absence of DSA(s), and the strength/trend of the DSA(s).

• Panel Reactive Antibody Specific Class 1 and 2

Not applicable

Luminometry

Special Test

• No patient preparation necessary.
• Pease get form to Special Test Section
• Fill up the DONOR and RECIPIENT FORM 
• Call Special Test Section (By Appointment/Schedule).

NOTE: Do not collect specimen without approval of Special Test Section

Red tube only

2 mL Serum

Not Applicable

Not applicable

Not applicable

5 days

Transport specimen with dry ice

• Hemolyzed specimen
• Markedly lipemic specimen
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used

Tuesday 7:00 AM

Monday 7am

Friday 5:00 PM

Available upon request

None specified.

Not applicable

for post-transplant monitoring and consists of HLA Class I & II Panel Reactive Antibody (PRA), the presence/absence of DSA(s), and the strength/trend of the DSA(s).

• Panel Reactive Specific Class 2

Not applicable

Luminometry

Special Test

•    No patient preparation necessary.
• Pease get form to Special Test Section
• Fill up the DONOR and RECIPIENT FORM 
• Call Special Test Section (By Appointment/Schedule).

NOTE: Do not collect specimen without approval of Special Test Section

Red tube only.

2 mL Serum

Not Applicable

Not applicable

Not applicable

5 days

Transport specimen with dry ice

• Hemolyzed specimen
• Markedly lipemic specimen
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used

Tuesday 7:00 AM

Monday 7am

Friday 5:00 PM

Available upon request

None specified.

Not applicable

for post-transplant monitoring and consists of HLA Class I & II Panel Reactive Antibody (PRA), the presence/absence of DSA(s), and the strength/trend of the DSA(s).

• TTR

Not applicable

• Transthyretin or Pre Albumin is a protein secreted by the liver which transports thyroid hormones and also, in a tertiary complex with Retinol Binding Protein, Vitamin A.

• Transthyretin levels are low in malnutrition, liver disease, inflammation, hyperthyroidism, hyperestrogenemia, and if it is being lost by leakage into the urine. Its levels are high in renal glomerular insufficiency and hypothyroidism and in response to corticosteroid therapy, high androgen levels and re-alimentation.

Nephelometry

Special Test

No patient preparation necessary.

Red or Gold tube

5 mL Serum

7 Days

30 Days

6 Months

Transport specimen at 2 – 8 °C (with cold packs)

•Quantity not sufficient

• Received room temperature

•Improperly labeled specimen

• Hemolyzed sample

Batch Running 

Friday 4:00 PM

14 Working days After Cut-off (excluding Saturdays, Sundays and Holidays)

Available upon request

None specified

Not applicable

Not Applicable

Not Applicable

To determine a baby’s gender as early as 10 weeks of gestation.

Genetic testing of fetal cells in maternal’s blood to determine the presence of Y-chromosome.

Special Test

1. General Inquiries can be entertained by In-house Doctor who attended the training. Doctor should inform patient to consult with her personal Physician regarding this test.
2. Patients must be at least 10 weeks in gestation
3. Inform Special Test Section 1 day before the scheduled sample collection.
4. Inform Special Test Section before the scheduled sample collection for the collection kit.

Sample collection/handling:

1. Each collection kit (provided by HP Main Lab) includes 2 WhiteGray top tubes and 1 Pink/Purple top tube.
2. Collect sample using the 2 Gray top tube (with gel separator)
3. Mix sample by gentle inversion.
4. Centrifuge sample and transfer plasma into the Pink/Purple top tube.
5. NOTE: Plasma from the 2 mother tubes shall be mixed together into the Pink/Purple top tube.
6. Completely fill up the required information in the Submission form.
7. Send sample immediately to HP Main Lab.

Prenatal Gender Test Kit will be provided by Special Test Section.

 

4 mL Plasma

Not Applicable

Not Applicable

5 Days

Not Applicable

Transport specimen at 2 – 8 °C (with cold packs)

• Hemolyzed specimen
• Markedly lipemic specimen
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used

Batch running

Monday, 12:00 PM

2 weeks after cut-off (excluding Saturdays, Sundays and Holidays)

Not Applicable

None Specified.

Not Applicable

Prenatal sex discernment, prenatal testing for discerning the sex of a fetus before birth

Not Applicable

Not Applicable

Used to establish paternity while pregnant. Can be performed as early as 9 weeks after conception.

Next-Generation Sequencing (NGS) and Single Nucleotide Polymorphism (SNP)

Special Test

• No patient preparation necessary.
• Request collection kit from Speciqal Test Section, 3 days before the collection day.
• Follow collection procedure provided in the collection kit.
• Inform Special Test Section 1 day before the scheduled sample collection.
• Sample collection can only be done every Monday only up to 12:00 PM

Document Requirements:

• Photocopy of Valid Government I.D of alleged father and mother.(visible complete name and date of birth)
• If patients are under 18 years of age, include a recent photograph of the patient.
• Completely filled up Prenatal Identification Form (Chain of Custody)

NOTE: Double check completeness of information in the required documents prior to sending to HPD Main Laboratory to avoid delay in processing of sample.

Non-invasive Prenatal Paternity Blood Collection Kit(Provided by Special Test)
 

20 mL whole blood from mother (black and brown tops)
4 pcs buccal swab from alleged father o

Not Applicable

Not Applicable

5 Days

Not Applicable

Transport specimen at 15°C ~25 °C (room temperature)

 

• Hemolyzed specimen
• Markedly lipemic specimen
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used

Batch Running

Monday, 12:00 PM

3 weeks after cut-off  (excluding Saturdays, Sundays and Holidays)

Not Applicable

None Specified

Not Applicable

Non-invasive prenatal DNA paternity testing, amniocentesis

• Spirometry with pre-post bronchodilator testing
• Post BD or Post Bronchodilator testing

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IMAGING DEPARTMENT

BRANCHES & SCHEDULE

No need for appointment, available for walk-in patients

• HPD Del Monte

NOTE: Please note that availability is subject to change without prior notice. Call the branch for confirmation.

PREPARATIONS

• Wear comfortable clothing that will not restrict breathing. 
• Do not eat a heavy meal prior to the test.
• Avoid smoking and vigourous exercise on the day of the test.
• Patient must inform his/her requesting physician if he/she is taking or on lung-related medications (e.g. inhaler) as this may be withheld prior to the test.

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How is Spirometry performed?

1. The patient will be asked to place his/her mouth into a mouthpiece that is attached to the spirometer, the lips should form a tight seal so air will not escape the spirometer. The patient will also wear a soft clip on the nose to keep air from leaking out of it.
2. Breathe normally until the lungs are empty.
3. Then, take a big, deep breath to fill up the lungs completely and fully.
4. Blow the air as hard and as fast as you can until the lungs are absolutely empty.
5. Repeat the test until there are three good efforts.

How is Spirometry with Pre-Post Bronchodilator testing performed?

1. Perform spirometry.
2. Administer the bronchodilator (i.e. Salbutamol) medication.
3. Wait for 15-20 minutes, and then perform spirometry again.

Is Spirometry painful?

• The test itself is painless. However, because the test requires the patient to breath in and out quickly, there is a possibility that he/she may experience some shortness of breath, chest discomfort, lightheadedness, or headache. It is best to communicate with the staff if these symptoms are experienced.

Can I catch an infection from doing Spirometry?

• Micro filters are used per patient so he/she will never breathe the air exhaled by another person.

• Prealb
• PREA
• Thyroxine binding prealbumin
• PA
• Transthyretin

Not Applicable

Prealbumin test may be used to find out if you are getting enough nutrients, espesially protein, in your diet. May also help diagnose certain infections and chronic diseases.

Immunoturbidemetric Assay

Chemistry

No patient preparation necessary.

Gold and Red tube

1-3 mL Serum

1-3 mL Plasma ( Li-Heparin, K2-EDTA)

3 Days

6 Months

1 Year

Transport specimen at 2°C - 8°C (with cold packs)

• Failed Serum Index
• Quantity Not sufficient
• Improperly labeled specimen
• Exceed sample stability requirement
• Improper collection tube used.

Saturday

Monday to Saturday: 6:00 PM

Sunday: 4:00PM

ROUTINE (on running day)
• 4 hours after receipt of specimen/ arrival of messenger
STAT(on running day only)
• After 2½ hours from extraction/messenger arrival

0.20-0.40 g/L (20.00-40.00 mg/dL)

• Intralipid causes artificially high prealbumin results.

• In very rare cases, gammopathy, in particular type IgM (Walderstrom's macroglobulinemia), may cause unreliable result.

Q: Why do i need a prealbumin blood test

A: Your doctor may order a prealbumin test to keep track of your nutrition if you are in the hospital. You may also need this test if you have sysmptoms of malnutrition. These include:

• Weight loss
• Weakness
• Pale, dry skin
• Brittle hair
• Boe and joint pain

Q: What do the results mean?

A: If your prealbumin levels are lower than normal, it may mean you are not getting enough nutrition in your diet. Low prealbumin levels may also be signs of:

• Trauma, such as burn injury
• Chronic illness
• Liver disease
• Certain infections
• Inflammation

High prealbumin levels may be a sign of Hodgkins disease, kidney problems, or other disorders, but this test is not used to diagnose or monitor conditions related to high prealbumin. Other types of lab tests will be used to diagnose these disorders.

If your prealbumin levels are not normal, it doesn't necessarily mean you have a condition needing treatment. Certain medicines and even pregnancy can affect your results. If you have questions about your results, always refer to your attending doctor.

Not Applicable

Pre-Eclampsia Screening for 1^stTrimester

• PlGF 1-2-3
• PAPP-A
• Risk Assessment for Pre-Eclampsia

Used as a screening test for pre-eclampsia of patients on their first trimester of pregnancy(single pregnancy only).

Time-resolved Fluoroimmunoassay

Immunology

• No patient preparation required.
• Draw blood during:1^stTrimester: 11^thto  14^thgestation weeks
• Documentation Requirement:Filled upPRE-ECLAMPSIA SCREENING TEST FORM (HPD_F_954).
• NOTE:Preferably, Ultrasound (CRL, UTPi) and MAP results to be filled up by Obstetrician, Gynecologist or Sonologist.

Red or Gold tube

1-3 mL Serum

Not applicable

PlGF 1-2-3:2days

PAPP-A:Not specified

NOTE: Must be separated from red cells or serum separature tube gel plugs.

PlGF 1-2-3:30 Days

PAPP-A:6 Days

NOTE: Must be separated from red cells or serum separature tube gel plugs.

PlGF 1-2-3:Not specified (no more than 6 freeze-thaw cycles)

PAPP-A:Not specified (Freeze only once)

NOTE: Must be separated from red cells or serum separature tube gel plugs.

• Separate the serum from clot or gel separator ASAP after centrifugation.
• Transported specimen at 2⁰C to 8⁰C (with cold packs)

• Improperly labeled specimens
• Hemolyzed specimens
• Lipemic specimens
• Improper collection tube used
• Specimen transported outside the required temperature.
• Quantity not sufficient

1st And 3rd Monday Of The Month

Friday, 6:00PM

Blood markers (PlGF and PAPP-A):3 Days After Running Day

Risk Assessment of Pre-Eclampsia Result:3 Days After Running Day

• Result not available online
• Patient will be the one to pick up result.
• Soft copy will be saved in HPD Branch Special Test result shared folder.

Low Risk

• Samples containing heterophilic antibodies give falsely elevated results
• Bicarbonate and HIGH Protein concentrations interferes with the assay
• PLASMA samples is NOT acceptable
• Complement activation may in some rare cases give falsely low results

Q: Who should be screened for pre-eclampsia?

A: All pregnant women should be assessed early on in their pregnancy to prevent the development of pre-eclampsia. They should also have access to screening even if there are no maternal risk factors or history of pre-eclampsia.

Q: Why does all pregnant women need to be assessed for pre-eclampsia?

A: The benefit of detecting and treating pre-eclampsia early in the pregnancy always outweighs the conventional wait-and-see approach to pre-eclampsia management.

Q: What should women know?

A: While pre-eclampsia screening is critical to protecting the health of mother and child, many women are unaware of pre-eclampsia or combined pre-eclampsia screening with the PlGF 1-2-3 assay. They need to know that pre-eclampsia can affect any pregnancy. They should also be informed that some pregnancies are more at risk of developing pre-eclampsia than others. Combined pre-eclampsia screening with the PlGF 1-2-3 assay is an effective way to assess this risk. In fact, women with a history of pre-eclampsia have a three to four times greater risk of developing chronic hypertension than mothers with no history of pre-eclampsia and double the risk of ischemic heart disease, venous thromboembolism and stroke.

Q: What do the results mean?

A:Low risk- Low risk means that there is minimal risk of developing pre-eclampsia later in pregnancy. While it is possible to develop pre-eclampsia regardless of low risk status, the pregnancy can continue as normal and the mother can rest assured that there is little or no reason for concern.

High risk– If the risk of developing pre-eclampsia later in pregnancy is high, the doctor can start treatment at the optimum time and monitor the pregnancy more closely. While not all women in the high-risk group develop pre-eclampsia, doctors can now offer the best possible care early in the pregnancy and significantly improve the outcome for mother and child:

• Trimester 1 – Start preventive actions
• Trimester 2 –Close monitoring
• Trimester 3 – Prepare for early delivery and needed actions.

Not applicable

Pre-Eclampsia Screening for 2^ndand 3^rdTrimester

• PlGF 1-2-3
• Risk Assessment for Pre-Eclampsia

Used to screen risk for pre-eclampsia of patients on their second/third trimester of pregnancy(single pregnancy only)

Time-resolved Fluoroimmunoassay

Immunology

No patient preparation required.

Draw blood during:

• 2^ndTrimester: 20^thto  27^thgestation weeks
• 3^rdTrimester: 28^thto 40^thgestation weeks

Documentation Requirement:Filled upPRE-ECLAMPSIA SCREENING TEST FORM (HPD_F_954).

NOTE:Preferably, Ultrasound (CRL, UTPi) and MAP results to be filled up by Obstetrician, Gynecologist or Sonologist.

Red or Gold tube

1-3 mL Serum

Not applicable

2 Days

NOTE: Must be separated from red cells or serum separature tube gel plugs.

30 Days

NOTE: Must be separated from red cells or serum separature tube gel plugs.

Not specified (no more than 6 freeze-thaw cycles)

NOTE: Must be separated from red cells or serum separature tube gel plugs.

• Separate the serum from clot or gel separator ASAP after centrifugation.
• Transported specimen at 2⁰C to 8⁰C (with cold packs)

• Improperly labeled specimens
• Hemolyzed specimens
• Lipemic specimens
• Improper collection tube used
• Specimen transported outside the required temperature.
• Quantity not sufficient

1st And 3rd Monday Of The Month

Friday, 6:00PM

Blood markers (PlGF and PAPP-A):3 Days After Running Day

Risk Assessment of Pre-Eclampsia Result: 3 Days After Running Day

• Result not available online
• Patient will be the one to pick up result.
• Soft copy will be saved in HPD Branch Special Test result shared folder.

Low Risk

• Samples containing heterophilic antibodies give falsely elevated results
• Complement activation may in some rare cases give falsely low results

Q: Who should be screened for pre-eclampsia?

A: All pregnant women should be assessed early on in their pregnancy to prevent the development of pre-eclampsia. They should also have access to screening even if there are no maternal risk factors or history of pre-eclampsia.

Q: Why does all pregnant women need to be assessed for pre-eclampsia?

A: The benefit of detecting and treating pre-eclampsia early in the pregnancy always outweighs the conventional wait-and-see approach to pre-eclampsia management.

Q: What should women know?

A: While pre-eclampsia screening is critical to protecting the health of mother and child, many women are unaware of pre-eclampsia or combined pre-eclampsia screening with the PlGF 1-2-3 assay. They need to know that pre-eclampsia can affect any pregnancy. They should also be informed that some pregnancies are more at risk of developing pre-eclampsia than others. Combined pre-eclampsia screening with the PlGF 1-2-3 assay is an effective way to assess this risk. In fact, women with a history of pre-eclampsia have a three to four times greater risk of developing chronic hypertension than mothers with no history of pre-eclampsia and double the risk of ischemic heart disease, venous thromboembolism and stroke.

Q: What do the results mean?

A:Low risk- Low risk means that there is minimal risk of developing pre-eclampsia later in pregnancy. While it is possible to develop pre-eclampsia regardless of low risk status, the pregnancy can continue as normal and the mother can rest assured that there is little or no reason for concern.

High risk– If the risk of developing pre-eclampsia later in pregnancy is high, the doctor can start treatment at the optimum time and monitor the pregnancy more closely. While not all women in the high-risk group develop pre-eclampsia, doctors can now offer the best possible care early in the pregnancy and significantly improve the outcome for mother and child:

• Trimester 1 – Start preventive actions
• Trimester 2 –Close monitoring
• Trimester 3 – Prepare for early delivery and needed actions.

Not Applicable

• Rapid hCG test (Serum)
• Rapid Serum PT

•Qualitative HCG (serum)
 •Human Chorionic Gonadotropin Rapid test (Serum)

Not Applicable

Used for the detection of pregnancy.

Immunochromatography

Serology

No patient preparation necessary.

Red or Gold tube

1-2mL Serum

Not applicable

Not Specified

7 Days

>2 Weeks

Transport specimen at 2 – 10 °C (with cold packs)

• Contaminated and markedly lipemic serum
• Improperly labeled specimens
• Hemolyzed specimens
• Quantity not sufficient
• Specimen stored exceeded outside required stability

Daily

Monday to Saturday
• 8:00 PM

Sunday
• 3:00 PM

ROUTINE
• 3 hours after receipt of specimen/ arrival of messenger
STAT
• 1½ hours from extraction/messenger arrival

Not applicable

• hCG has been found in patients with both gestation and non-gestation trophoblastic diseases. These conditions should be ruled out when interpreting hCG levels.
• hCG is also detected after pregnancy, Caesarian section, miscarriage and abortion.

Not Applicable

Pregnancy, Qualitative hCG; Quantitative hCG; Beta hCG; Total hCG; Total beta hCG, Human Chorionic Gonadotropin, Progesterone, First Trimester Down Syndrome Screen, Second Trimester Maternal Serum Screening, Panorama Prenatal Screening Test

• Rapid hCG Test (Urine)
• Rapid Urine PT
• Qualitative HCG (Urine)
• Human Chorionic Gonadotropin Rapid test (Urine)
• HCG Pregnancy (Urine)
• Beta HCG  (Urine)

Not applicable

Test specific for hCG beta subunit and offers sensitivity necessary to detect pregnancy as early as ten (10) days after conception.

Double Antibody Sandwich and Immunochromatography Method

Clinical Microscopy

First morning urine specimen is preferred since it generally contains the highest concentration of hCG; however, urine specimens collected at any time of the day may also be used.

Clean, leak-proof container

• 10-15 ml urine (if with urinalysis)
• 5 ml urine(if only pregnancy test)

Not applicable

4 Hours

Not specified.

NOTE: Once taken out of the refrigerator, urine samples must be submitted to laboratory within 1 hour with cold packs.

Not applicable

Transport specimen at 2 – 8 °C (with cold packs)

• Quantity not sufficient
- Sample less than 10mL in volume (if with urinalysis)
- Sample less than 5mL in volume (If only pregnancy test)

Daily

Monday to Saturday
• 8:00 PM

Sunday
• 4:00 PM

ROUTINE
• 3 hours after receipt of specimen/ arrival of messenger
STAT
• After 1½ hours from receipt of specimen/ arrival of messenger

Not applicable

None identified.

Q: How many days from the missed menstrual period can hCG be detected in human urine?
A: Atleast 5 -15 days after the first missed period.

Progesterone, Panorama Prenatal Screening

• 5P
• 5-Pregnenolone
• Delta-5-pregnenolone

Not applicable

An adrenal androgen which is converted either into DHEA or DHEA-Sulfate by the action of 17,20 desmolase or into 17-OHP by the action of 3 betaOL-dehydrogenase. Useful in the investigation of hyperandrogenism due to 3 betaOL-dehydrogenase deficiency.

Radio Immuno Assay (RIA)

Special Test

No patient preparation necessary.

Red or Gold Tube

5 mL Serum

Not applicable

24 Hours

7 Days

28 Days

Transport specimen at 2 – 8 °C (with cold packs)

• Hemolyzed specimen
• Markedly lipemic specimen
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used

Batch Running

Friday, 4:00 PM

2 months after cut-off (excluding Saturdays, Sundays and Holidays)

Available upon request

None specified.

Not applicable

P5, an endogenous steroid hormone

T Cell

• CD 3
• CD 4
• CD 8

Flow Cytometry

Special Test

• No patient preparation necessary.
• Call Special Test Section (By Appointment/Schedule).

NOTE: Do not collect specimen without approval of Special Test Section

EDTA or Violet Tube

3 pcs of 4 mL whole blood

Not Applicable

6 Hours

Not Applicable

Not Applicable

Transport specimen at 15°C ~25 °C (room temperature)

• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used
• Clotted Sample
• Over-filled or Under-filled tube

Monday to Friday

Monday to Friday, 7:00 AM

7 days after running (excluding Saturdays, Sundays and Holidays)

Available upon request

None Specified.

Not Applicable

Immune system is missing or does not function normally