Laboratory Test Preparation

• Aspirin
• Acetylsalicylic acid
• Bismuth subsalicylate
• • Methyl Salicylate
• Sodium Salicylate
• Salsalate
• Magnesium Salicylate

NoT Applicable

Salicylate is used in the treatment of fever, analgesia and in the treaatment of acute rheumatic fever, rheumatoid arthritis and for inhibition of platelet aggregation in patients with CAD.

Monitored to assess toxicity.

Spectrometry

Special Test

• No patient preparation necessary.

Note the following info:

• Dosage
• Date and Time of last dose intake
• Date and Time of extraction

Red Top only (No Gel separator)

3 mL Serum

Not Applicable

8 Hours

14 Days

Not Applicable

Transport specimen at 2 – 8 °C (with cold packs)

• Hemolyzed specimen
• Markedly lipemic specimen
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used

Monday to Friday

Monday to Friday, 7:00 AM

3 Days (except Saturdays, Sundays and Holidays)

Available upon request

Not applicable

• SARS-CoV-2 (CPVID19) RT-PCR
• Severe Acute Respiratory Syndrome Coronavirus 2 (SARS CoV-2) RNA Detection by RT-PCR
• Coronavirus 2019 Test RT-PCR
• SARS CoV-2 Test RT-PCR
• COVID-19 RT-PCR
• 2019-nCoV RT-PCR

Not Applicable

Use for real-time PCR detection of Sever Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) viral RNA, the causative agent of COVID-19

Real-Time Polymerase Chain Reaction (RT-PCR)

Molecular Diagnostics

A. Documentation Requirements:

1. A doctor's request ismandatory.No doctor's request, no testing allowed. soft copy of doctor's request/screenshot from mobile phone is acceptable.
2. Patient is required to submit a completely filled outCase Investigation Form (CIF).
3. Patient must present a valid ID prior to swabbing.
4. Send in clients, branches, home service and mission personnel are required to submit completely filled upTABULATED REQUEST FORM FOR SARS-CoV-2 RT PCR TESTING (HPD_f_1067)

B. Specimen Collection:

1. For walk in patients, sample collection is available by schedule in selected branches (please check website for contqact details and branches available for collection).
2. For specimen collector's: Wear proper PPEs and practice safety procedures in handling specimens.
3. If the patient has a lot of mucous in his/her nose, this can interfere with the collection of cells. Patient should use a tissue to gently clean out visible nasal mucous before a swab is taken. Discard tissue used as biohazard waste.

1. Wash hands.
2. Put on appropriate PPE (head cap, goggles/face shield, face mask, disposable lab gown and double gloves)
3. Label tube of viral tube media (VTM) legibly with:

1. Patient's full name (last name, first name, with middle name)
2. Date and Time of collection
3. Specimen type: "NP" for nasopharyngeal swab, "OP" for oropharyngeal swab, or "NP & OP" for both
4. Write the Date and Time of collection and specimen type on the request form as well.

4. Packaging and Transport:Follow triple packaging protocol.

1. After specimen collection, ensure that covers of viral tube media (VTM) are tightly closed. Additionally, use parafilm or cling wrap to seal the primary specimen container (VTM)
2. Decontaminate the outer part of the primary specimen container using 10% Bleach solution as disinfectant. Be cautious not to remove the label.
3. c. After specimen collection, ensure that covers of viral tube media (VTM) are tughtly closed. Additionally, use parafilm or cling wrap to seal the primary specimen container.
4. Decontaminate the outer part of the primary specimen container using 10% Bleach solution as disinfectant. Be cautious not to remove the label.
5. Wrap the primary specimen container with absorbent material (cotton, tissue or gauze).
6. Individually, put the primary specimen container in a transparent zip-lock bag then seal the bag. One zip-lock bag per specimen.
7. Disinfect external side of the zip-lock bag (containing the wrapped primary specimen container) with 10% Bleach solution.
8.  Place it inside the secondary receptacle/container and properly close tightly. Disinfect the secondary container and place it inside the outer container.
9. Place 4-6 ice packs, one at the bottom, all four sides and at the top of the secondary container. Maintain refrigerated temperature (2-10°C).
10. Seal the outer container properly and disinfect the outside of the container.
11. Place the necessary labels (shipper and sender’s details) and forms (request forms) in separate zip lock bags and seal tightly. Securely tape the labels and forms outside the box
12. Send to laboratory within 72 hours after collection.

Note:If sample cannot be sent to laboratory immediately, store sample at refrigerated temperature (2-8°C).

Viral Tube Media (VTM)

1 nasopharyngeal swab (swabbed from both nostrils)AND1 oropharyngeal swab placed in one viral tube media (VTM)

1-2 nasopharyngeal swab (swabbed from both nostrils)

Not Applicable

3 days (72 hours)

Not Applicable

Transport specimen at refrigerated temperature (2-10'C)

Note: Send to laboratory with 72 hours after collection.

• Exceed sample stability requirement.
• Sample not properly packed (did not follow triple package requirement)
• Sample with leak
• Improperly labeled specimen
• Improper collection tube used
• Shipment with Request forms not placed outside the outer container
• Incompletely filled up CIF and Tabulated Request Form (pease see attached memo from DOH)
• Pnly 1 Oropahyngeal swab collected.

Monday to Sundays

6:00 PM

• Routine : 48 -72 hours
• STAT: 24 hours

Note: STAT requests will be received fromMonday up to Saturday 6PM ONLY

Test Results: SARS-CoV2 (causative agent of COVID-19) viral RNA Detected
Interpretation:POSITIVE for SARS-CoV2 (causative agent of COVID-19)

Test Results:SARS-CoV2 (causative agent of COVID-19) viral RNA NOT Detected
Interpretation: NEGATIVE for SARS-CoV2 (causative agent of COVID-19)

Test Results: Invalid due to specimen quality
Interpretation:Negative for test internal control (most likely due to poor specimen quality)

• The product test results are for clinical reference only and should not be the sole basis for clinical diagnosis and treatment. The clinical management of patients should be considered in combination with clinical symptoms/signs, medical history, other laboratory detection, treatment responses, epidemiology and etc.
• Suspected samples are recommended to be retested at intervals.
• The accuracy of the test may be affected by sample collection and storage. High temperature and direct sunlight should be avoided.

Q: Can blood be collected for PCR testing?

A: No, acceptable specimens for SARS-CoV-2 Virus PCR testing are nasopharyngeal and oropharyngeaql swabs placed in a viral tube media.

Q: How is a SARS-CoV-2 Virus PCR test different from COVID-19 IgG/IgM or Total Antibody

A: SARS-CoV-2 Virus PCR test detects the presence of the virus itself while COVID-19 IgG/IgM or Total Antibodydetects antibody to the virus.

Q: Who can prescribe and interpret SARS-CoV-2 Virus PCR test result?

A: ONLY medical doctors can prescribe and interpret result of SARS-CoV-2 Virus PCR test.

Q: What will I do if my test is positive for SARS-CoV-2 Virus PCR test?

A: Anyone who tests positive for SARS-CoV-2 Virus PCR test should immediately consult a doctor and quarantine himself.

Q: Who are at higher risk in having severe illness from COVID-19?

A: Older adults and people of any age who have serious underlying medical condition may be at higher risk for more severe complications from COVID-19.

2019-nCoV RT-PCR; Corona virus disease; SARS-CoV-2; COVID-19

Not applicable

Not applicable

Schistosomiasis affects over 250 million people in the world. Intestinal schistosomiasis is caused by Schistosoma mansoni in sub-Saharan Africa, the Middle East and South America, and by S. japonicum in the Far East. Intestinal infection causes colitis and, in some cases, the liver and spleen become involved which can lead to fibrosis and cirrhosis. In the Middle and Far East, and in Africa, S. haematobium causes vesical schistosomiasis, the main symptom of which is hematuria. Adult flukes live in the venous plexus and, after crossing the epithelial barrier, their eggs are shed in either the urine or the feces. Diagnosis depends on detecting the eggs but serological analysis can be of use either as a second-line option if very few flukes are present or for post-therapeutic monitoring purposes.

Enzyme-immunoassay

Special Test

No patient preparation necessary.

Red or Gold Tube

3 mL Serum

Not Applicable

7 days

14 days

30 days

Transport specimen at 2 – 8 °C (with cold packs)

• Hemolyzed specimen
• Markedly lipemic specimen
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used

Batch Running

Friday 4:00 PM

14 working days after cut-off (excluding Saturdays, Sundays and Holidays)

Not Applicable

None specified.

Not applicable

MARKERS IN PROGRESIVE SYSTEMIC SCLEROSIS(SCLERODERMA)

• anti-Scl-70                
• anti-Centromères A (CENP A)
• anti-Centromères B (CENP B)
• anti-ARN polymérase III (11)
• anti-ARN polymérase III (155)
• anti-fibrillarin          
• anti-NOR90                 
• anti-Th/To                 
• anti-PM100                 
• anti-PM75                  
• anti-Ku                    
• anti-PDGFR                  
• anti-Ro52     

Systemic sclerosis (SSc) is an autoimmune disease characterized by vascular abnormalities, and cutaneous and visceral fibrosis. Some antibodies are considered highly specific for SSc, including Scl-70 (anti-topoisomerase 1) antibody, anti-centromere (CENP) antibodies, and anti-RNA polymerase III antibody and are classification criteria for disease defined by the American College of Rheumatology. Centromere antibodies are found in patients with a limited form of cutaneous systemic sclerosis, "CREST" syndrome, a complex of subcutaneous calcinosis, Raynaud's phenomenon, esophageal dysmotility, sclerodactyly, and telangiectasias. 

Immunodot

Special test 

Not applicable 

Gold / Red tube

3ml Serum 

Not applicable 

4 Days

7 Days

30 Days

Transport specimen at 2 – 8 °C (with cold packs)

 Hemolyzed specimen

• Markedly lipemic 

• Exceeded sample stabilityrequirement

• Quantity Not Sufficient

• Improper labelled specimen

• Improperly collection tube used

Batch running

Friday 4:00 PM

4 Working Weeks after cut-off (excluding saturdays, Sundays and Holidays)

Not applicable 

Not applicable 

Not applicable 

Not applicable 

Ricketttsia tsutsugamushi

Not applicable

• Rickettsiae responsible for typhus. Exanthemic typhus, caused by R. prowazekii, transmitted by lice is a disease associated with war and poverty. It is tending to die out but may resurge. Murine thymus, caused by R. typhi (or mooseri) transmitted by rat fleas, is an attenuated form of the above disease. It is found in some cosmopolitan ports.
• Diagnosis is based on serological analysis demonstrating seroconversion with specific IgM.

Immunoflourescence

Special Test

No patient preparation necessary.

Red or Gold Tube

3 mL Serum

Not Applicable

7 Days

14 Days

30 Days

Transport specimen at 2 – 8 °C (with cold packs)

• Hemolyzed specimen
• Markedly lipemic specimen
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used

Batch Running

Friday 4:00 PM

2 weeks after cut-off (excluding Saturdays, Sundays and Holidays)

Available upon request

None specified.

Not applicable

Bush typhus, Intracellular parasite Orientia tsutsugamushi

• Silica Clotting Time
• Kaolin Clotting Time (KCT)

Not applicable

• Used to help in the diagnosis and/or assess a person with an autoimmune disease associated with Systemic Lupus Erythematosus (SLE).
• For autoimmune disorder that is associated with pregnancy

Hematology

• No patient preparation necessary.
• DO NOT use glass collection and transport tube

Citrated Blue top

at least 1mLCitrated Plasma

Not applicable

4 Hours

Not applicable

7 Days

• Improper use of anticoagulant
• Insufficient volume of blood to anticoagulant
• Improperly labeled specimens
• Hemolyzed specimens
• Specimen stored and transported outside the temperature requirement

Wednesday and Saturday

Wednesday and Saturday 2PM

Thursday and Sunday 9AM

1.08-1.21 (ratio)

• Dysfibrinogenemia
• Heparin intake
• Intrinsic pathway deficiencies
• Presence of factor inhibitors
• Hepatic disorders
• Treatments with vitamin K antagonists
• Treatment with thrombin inhibitors
• DIC

Not applicable

Phospholipid-dependent, Coagulation assay , Lupus Anticoagulant (LA) antibodies, DRVVT, SLE, ANA, Antiphospholipid syndrome, Pregnancy, Miscarriage,Partial Thromboplastin Time (PTT, aPTT),Thrombin Time,Antithrombin,Cardiolipin Antibodies,Beta-2 Glycoprotein 1 Antibodies,Factor V Leiden Mutation and PT 20210 Mutation,Homocysteine,Protein C and Protein S,Excessive Clotting Disorders,Deep Vein Thrombosis (DVT).

Se

Not Applicable

Selenium is considered an essential trace element. It enters the food chain through plants. Selenium plays an important role in the control of thyroid hormone metabolism. Selenium deficiency may cause reduced growth rates, owing to a feedback response, which lowers tri-iodothyronine-mediated synthesis of growth hormone in the pituitary, while a combined deficiency of selenium and iodine exacerbates hypothyroidism. Selenium is important for proper reproductive performance. Selenium testing is used to investigate for selenium deficiency or rarely to investigate for selenium toxicity. Selenium is often measured along with other trace elements (e.g iron, zinc & copper) to identify deficiencies where a person may benefit from supplementation.

A trace element which plays a key role in antioxidation mechanisms. Workers in the glass, dye and electronics industries, and those who use fungicides may be at risk of overexposure.

Inductively Coupled Plasma/Mass Spectrometry (ICP-MS)

Special Test

No patient preparation necessary.

Sodium heparin tube (do not use tubes with separator gel)

4 mL Plasma

Not Applicable

8 Hours

7 Days

28 Days

Transport specimen at 2 – 8 °C (with cold packs)

• Hemolyzed specimen
• Markedly lipemic specimen
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used

Batch Running

Friday, 12:00 PM

3 weeks after cut-off (excluding Saturdays, Sundays and Holidays)

Available upon request

None Specified.

Not Applicable

Glutathione peroxidase, other trace elements, copper, zinc, manganese, iron

• Se
• Selenium, Random Urine w/ Creatinine

• Selenium Urine
• Creatinine, Random Urine
• Selenium/Creatinine Ratio 

Selenium is considered an essential trace element. It enters the food chain through plants. Selenium plays an important role in the control of thyroid hormone metabolism. Selenium deficiency may cause reduced growth rates, owing to a feedback response, which lowers tri-iodothyronine-mediated synthesis of growth hormone in the pituitary, while a combined deficiency of selenium and iodine exacerbates hypothyroidism. Selenium is important for proper reproductive performance. Urinary selenium concentrations are used as an indicator of selenium status.

Selenium is considered an essential trace element. It enters the food chain through plants. Selenium plays an important role in the control of thyroid hormone metabolism. Selenium deficiency may cause reduced growth rates, owing to a feedback response, which lowers tri-iodothyronine-mediated synthesis of growth hormone in the pituitary, while a combined deficiency of selenium and iodine exacerbates hypothyroidism. Selenium is important for proper reproductive performance.

Inductively Coupled Plasma/Mass Spectrometry (ICP/MS)

Special Test

• Request Container to Special test section (Acid Washed Container)
• Avoid seafood consumption for 48hours prior to collection 

Acid Washed Container

60mL Urine in Acid Washed Container

Not Applicable

24 Hours

7 Days

28 Days

Transport specimen at 2 – 8 °C (with cold packs)

• Quantity not sufficient
• Received room temperature
• Improperly labeled specimen
• Improper urine collection
• If specimen submitted in a non-acid washed container

Batch Running

Monday, 12:00 PM

2 weeks after cut-off (excluding Saturdays, Sundays and Holidays)

Available upon request

None specified.

Not applicable

Glutathione peroxidase, other trace elements, copper, zinc, manganese, iron

• Sperm Testing /Semen Testing
• Sperm Analysis /Smen Analysis
• Seminal Fluid Analysis

 •Sperm Count / Semen Count

1. Macroscopic Examination:
• Liquefaction
• Viscosity
• Appearance
• Total Volume (mL)
• pH
2. Microscopic Examination:
• Round Cells Concentration
• Sperm Motility
• Sperm numbers
a. Sperm Concentration
b. Sperm Count
• Sperm Morphology

• Used as aid in diagnosis of male infertility
• Evaluation of post-vasectomy patients

World Health Organization (WHO) 2010

Clinical Microscopy

1. The sample should be collected after a minimum of 2 days and a maximum of 7 days of sexual abstinence.
2. The sample should be collected inside the facility of HPD in order to limit the exposure of the semen to fluctuations in temperature and to control the time between collection and analysis.

Pre-weighed clean wide mouth container provided by Hi-Precision Diagnostics Laboratory
*Note: Weight must be written at the bottom of the empty specimen container. Do not use container if weight is not noted.

> 1.5 mL
NOTE:
• Less than 1 mL sample is acceptable provided that full amount of ejaculate is completely collected.
• Spillage of sample is strictly not allowed.

Not applicable

Not applicable

Not applicable

Not applicable

Send sample immediately to laboratory for analysis (should be less than an hour after collection at 37°C)

1. Improperly labeled specimen container
2. Specimen delivered to the laboratory more than one hour after collection.
3. Incomplete collection of semen sample. E.g. spillage of sample and/or incomplete abstinence.

Daily

Monday to Saturday
• 6:00 PM

Sunday
• 3:00 PM

ROUTINE
• 5 hours after receipt of specimen/ arrival of messenger
 

NO STAT

Available upon request.

1.Incompletely collected/ Sample collected in unsuitable containers may lead to erroneous results. 2. High viscosity can interfere with determination of sperm motility and sperm concentration .

Q: How many days of abstinence is required?
A: 2-7 Days

Male fertility testing

• 5-Hydroxytryptamine
• Hydroxytryptamine
• 5-OHT

Not Applicable

 Serotonin concentrations are greatly increased in patients with carcinoid syndrome. Carcinoid tumors are associated with Multiple Endocrine Neoplasia (MEN) Types I and II. These tumors are associated with flushing, diarrhea, pain, and other symptoms.

Fluorescence Detection • High Performance Liquid Chromatography (HPLC)

Special Test

DIET: within 24 hours prior to the assay. Avoid consuming the following food below: 

• Bananas
• Tomato
• Avocado
• Walnuts and pecans
• Eggplant
• Plums
• Pineapple

Gold or Red Tube

3 mL Serum

Not Applicable

2 Hours

Not Applicable

28 Days

• Transport specimen at 2 – 8 °C (with cold packs) - 2 Hours
• Transport specimen at -20 °C (with cold packs) - More than 2 Hours

• Hemolyzed specimen
• Markedly lipemic specimen
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used

Batch Running

Monday, 12:00 PM

3 weeks after cut-off (excluding Saturdays, Sundays and Holidays)

Available upon request

Not applicable

• Serum Glutamic Oxaloacetic Transaminase
• GOT
• Aspartate Transaminase

Not applicable

Helps asses the liver function

IFCC with Pyridoxal Phosphate activation

Chemistry

No patient preparation necessary.

Red or Gold tube

1-3 mL Serum

1-3mL Plasma (Li-heparin and K2-EDTA)

4 Days

7 Days

3 Months

Transport specimen at 2 – 8 °C (with cold packs)

• Specimens that failed the serum index criteriaExceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used

Daily

MONDAY TO SATURDAY: 6:00 PM

SUNDAY:4:00PM

RUNNING TIME: 6:00 AM onwards

RELEASING TIME: ROUTINE
• 4 hours after receipt of specimen/ arrival of messenger
RELEASING TIME: STAT
• After 2½ hours from extraction/messenger arrival

MALE: 10.00~50.00 U/L
FEMALE: 10.00~35.00 U/L

• Cyanokit (Hydroxocobalamin) may cause interference with results.
• Physiological plasma concentrations of Sulfasalazine and Sulfapyridine may lead to false results.
• In very rare cases, gammopathy, in particular type IgM (Waldenstrom’s macroglobulinemia), may cause unreliable results.

Not applicable

ALT, ALP, GGT, Bilirubin, Liver Panel, Albumin, Total Protein, Comprehensive Metabolic Panel

• Serum Glutamic-Pyruvic Transaminase
• GPT
• Alanine Transaminase
• Alanine Aminotransferase

Not applicable

Helps asses the liver function

IFCC with Pyridoxal Phosphate activation

Chemistry

No patient preparation necessary.

Red or Gold tube

1-3 mL Serum

1-3mL Plasma (Li-heparin and K2-EDTA)

3 Days

7 Days

>7 Days (at -60 to -80 °C)

Transport specimen at 2 – 8 °C (with cold packs)

1. Specimens that failed the serum index criteria
2. Exceeded sample stability requirement
3. Quantity not sufficient
4. Improperly labeled specimen
5. Improper collection tube used

Daily

MONDAY TO SATURDAY: 6:00 PM

SUNDAY: 4:00PM

ROUTINE
• 4 hours after receipt of specimen/ arrival of messenger
STAT
• After 2½ hours from extraction/messenger arrival

MALE: 10.00~50.00 U/L
FEMALE: 10.00~35.00 U/L

• Cyanokit (Hydroxocobalamin) may cause interference with results.
• Physiological plasma concentrations of Sulfasalazine or Sulfapyridine may lead to false results.
• Calcium dobesilate can cause artificially low ALT results at therapeutic concentrations.
• In very rare cases, gammopathy, in particular type IgM (Waldenstrom’s macroglobulinemia), may cause unreliable results.

Not applicable

AST, ALP, GGT, Bilirubin, Liver Panel, Comprehensive Metabolic Panel, Albumin, Total Protein, Prothrombin Time, Acute Viral Hepatitis Panel

• Sex Hormone Binding Globulin
• Testosterone-estrogen Binding Globulin (TeBG)

Not applicable

May be used to help evaluate men for low testosterone and women for excess testosterone production. It may be ordered in conjunction with other tests to evaluate the status of a person's sex hormones.

Chemiluminescent Microparticle Immunoassay (CMIA)

Special test

No patient preparation necessary.

Red or Gold tube

3mL Serum

Not applicable

Not specified

3 Days

2 months (Freeze only once)

Transport specimen at 2°C – 8 °C (with cold packs)

• Hemolyzed specimen
• Markedly lipemic specimen
• Specimen stored/transported outside the temperature range
• Quantity not sufficient
• Improperly labeled specimens

Monday to Friday

Monday to Friday 7am

3 Working days Excluding Saturday, Sunday and Holidays

Not applicable

Not applicable

Not applicable

Not Applicable

Not Applicable

Test determines whether two individuals share one or both parents.

DNA Analysis

Special Test

• Hardcopy of RTPCR negative results for BOTH PARTIES are needed 2-3 days from the date of collection.
• Inform Special Test Section before the scheduled sample collection for the collection kit.
• Request DNA Collection Kit to Special test section 3 days prior of swab collection.
• Only trained doctors/RMTs are allowed to collect samples.

Sample collection/handling:

1. Each collection kit (provided by HP Main Lab) includes 4 swabs.
2. Collect sample using the 4 swabs for each patient (i.e. 4 swabs for Sibling 1, 4 swabs for Sibling 2)
3. Return swabs to its sterile package and place it inside the provided envelope.
4. Encircle whether sample came from Sibling 1 or Sibling 2.       NOTE:Sample from Sibling 1 and Sibling 2 must be placed in separate envelopes.
5. Completely fill up the required information in the envelope.      NOTE:Signatures of patient and collector is required. For minors, legal guardian must sign on the envelope containing the Sibling's sample.
6. Remove adhesive cover and seal the envelope with sample.
7. Send sample immediately to HP Main Lab.

Document Requirements:

• Birth Certificate/School I.D of Siblings actual and  2 photocopies
• Valid I.D of guardian, actual and  2 photocopies
• Completely filled up request form
• Completely filled up disclaimer form

NOTE:Double check completeness and correctness of information before sending to HP Main Lab to avoid delay ing processing of sample.

DNA Collection kit(provided by Special Test)

4 swabs each from Sibling 1 and Sibling 2

Not Applicable

7 Days

Not Applicable

Not Applicable

Transport specimen at 15°C ~25 °C (room temperature)
 

• Incomplete details of patient
• Unlabelled sample
• Incorrect collection container used
• Incomplete documents needed

Batch Running

Friday, 12:00 PM

2 weeks after cut-off (excluding Saturdays, Sundays and Holidays)

Not Applicable

None Specified.

Not Applicable