Test:Thyroxine Binding Globulin
Other Test Request Name:
  • TBG
  • Thyroid Binding Globulin

 

Test Composition:

Not applicable

Intended Use:

Thyroxine Binding Globulin (TBG) transports the thyroid hormones (all the triiodothyronine and 70% of the thyroxine) in the blood. The levels of TBG available impact directly on the bioavailability of the thyroid hormones. Increased TBG levels lead to increased total hormone and decreased free hormone levels, but this decrease is rapidly compensated when, in response to low levels, the hypothalamus stimulates the thyroid gland to increase its rate of secretion. In the opposite situation, low levels of TBG result in a reduced total thyroid hormone concentration in the blood with a corresponding increase in free hormone.TBG levels may be high in estrogenemia (e.g. in pregnant women, women taking estrogen-based products and in feminizing tumors), hyperproteinemia (e.g. in liver disease and porphyria) and in certain hereditary disorders.Low TBG levels are associated with congenital TBG deficiency, hyperadrenalism, virilizing tumors, acromegaly, nephrotic syndrome, shock and with the response to certain drugs (steroids,corticoides...)

Methodology:

Radioimmunoassay

 

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:

No patient preparation necessary.

Collection/Sample Container:

Red or Gold tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:

3 mL Serum

Alternative Specimen and Volume Requirement:

Not applicable

Specimen Stability  
•   Room Temperature (15-25°C):

24 Hours

•   Refrigerated Temperature (2-8°C):

7 Days

•   Freezer Temperature (-20°C):

28 Days

Transport Temperature:

Transport specimen at 2°C – 8 °C (with cold packs)

Rejection Criteria:
  • Hemolyzed specimen
  • Markedly lipemic specimen
  • Specimen stored/transported outside the temperature range
  • Quantity not sufficient
  • Improperly labeled specimens
Running Day:

Batch Running

Cut Off Time:

Friday, 4:00 PM

TAT/Releasing of Results:

2 months after cut-off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:Not Applicable
Limitations/Interferences:

None specified.

Frequently Asked Questions (FAQs):

Not applicable

Related Words/Test:

Thyroid Antibodies, Thyroid Autoantibodies; Antithyroid Antibodies; Antimicrosomal Antibody; Thyroid Microsomal Antibody; Thyroid Peroxidase Antibody; Thyroperoxidase Antibody; TPO; Anti-TPO; TBII; Antithyroglobulin Antibody; TgAb; TSH Receptor Antibody; TRAb; Thyrotropin Receptor Antibodies; Thyroid Stimulating Immunoglobulin; TSI, Thyroid Peroxidase Antibody; Thyroglobulin Antibody; Thyroid Stimulating Hormone Receptor Antibody

 

Test:TIBC with Iron
Other Test Request Name:

• Total Iron Binding Capacity with Fe

Test Composition:
  1. Total Iron Binding Capacity
  2. Iron
  3. UIBC
Intended Use:

The serum measurement of iron is useful in the differential diagnosis of anemia, iron deficiency anemia, thalassemia, possible sideroblastic anemia, and iron poisoning.

Methodology:

Iron :  Ferene

TIBC: Calculated based on the total value of UIBC and Iron.

Laboratory Section:

Chemistry

Special Instructions/Patient Preparations:
  • Sample should be taken in the morning before patient is given therapeutic iron or blood transfusion.
  • Iron determinations on patients who have had blood transfusions should be delayed several days.
Collection/Sample Container:

Red or Gold tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:

1-3 mL Serum

Alternative Specimen and Volume Requirement:

1-3 mL Plasma (Lithium Heparin or Sodium Heparin)

Specimen Stability  
•   Room Temperature (15-25°C):

7 Days

•   Refrigerated Temperature (2-8°C):

3 Weeks

•   Freezer Temperature (-20°C):

1 Year

 

NOTE:Serum must be separated from gel separator

Transport Temperature:
  • Uncentrifuged sample: Room temperature (15°C–25°C)
  • Refrigerated Temperature (2°C– 8°C) (with cold packs)

NOTE: Sample must be separated from serum within 2 hours of specimen collection

Rejection Criteria:
  • Specimens with any sign of hemolysis
  • Improper collection tube used
  • Specimen stored/transported outside the temperature range
  • Quantity not sufficient
  • Lipemic specimen
  • Improperly labeled specimen
  • EDTA plasma
Running Day:

Monday, Wednesday, Friday

Cut Off Time:

Monday to Saturday: 6:00 PM

Sunday: 4:00PM

TAT/Releasing of Results:

ROUTINE:

  • 4 hours after receipt of specimen/ arrival of messenger

STAT:

  • After 2½ HOURS from extraction/messenger arrival

 

Reference Interval/Result Interpretation:

Total Iron Binding Capacity: 44.80~71.60 umol/L (250.30~400.03 ug/dL)

Iron: Male:11.60~31.30 umol/L (64.81 - 174.87 ug/dL)

         Female:9.00~30.40 umol/L (50.28 – 169.85 ug/dL)

Limitations/Interferences:

Substances that may interfere with the assay:

  • Gadolinum Magnetic Resonance Contrast Agents
  • Deferasirox
Frequently Asked Questions (FAQs):

Not applicable

Related Words/Test:

Ferritin, TIBC, UIBC and Transferrin, Hemoglobin, Hematocrit, Complete Blood Count, Reticulocyte Count, Zinc Protoporphyrin, Iron Tests, Soluble Transferrin Receptor

 

Test:TIN (ICP-MS)
Other Test Request Name:

TIN, Total

Test Composition:

Not Applicable

Intended Use:

Occupational Health. The assay is not used frequently even in cases of occupational exposure. Tin binds with blood cells and is distributed throughout the skeleton.

Exposure monitoring/investigation.

To screen for, detect, and monitor excessive exposure to specific heavy metals.

Methodology:

Inductively Coupled Plasma/Mass Spectrometry (ICP/MS)

 

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:
  • Request Container to Special test section 
  • Carefully clean skin prior to venipuncture. Avoid worksite collection
Collection/Sample Container:

EDTA (Royal blue top) - (Request Container to Special test section)

 
Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:

3 pcs of  4mL EDTA wholeblood (royal blue-top) tube(Provided by Special Test)

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

24 Hours

•   Refrigerated Temperature (2-8°C):

7 Days

•   Freezer Temperature (-20°C):

28 Days

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:
  • Exceeded sample stability requirement
  • Quantity not sufficient
  • Improperly labeled specimen
  • Improper collection tube used
  • Clotted Sample
  • Over-filled or Under-filled tube
Running Day:

Batch Running

Cut Off Time:

Monday, 12:00 PM

 

TAT/Releasing of Results:

2 weeks after cut-off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Available upon request

Limitations/Interferences:

None Specified.

Frequently Asked Questions (FAQs):

Q: When to get tested? 

A: Periodically when you work with heavy metals, or when a healthcare practitioner suspects that you may have been exposed to one or more heavy metals

Q: How is the test used? 

A:Heavy metals testing is used to:

  • Screen for or to diagnose heavy metal poisoning in those who may have been acutely or chronically or exposed to one or more heavy metals
  • Guide management and treatment of heavy metal poisoning
  • Monitor excessive metal concentrations in those who work with various heavy metals; such occupations include construction work, mining, radiator repair shops, and firing ranges.
  • Monitor the effectiveness of chelation therapy a treatment to rid the body of high amounts of a heavy metal

As mentioned above, your healthcare practitioner may order the metals panel that corresponds to your occupation, hobby, suspected exposure, and/or clinical symptoms. Alternatively, if your healthcare practitioner suspects that you have been exposed to a specific metal, such as lead, your practitioner may order that specific test instead of, or in addition to, a panel. Lead is usually ordered by itself when screening for exposure, especially in children because of how susceptible they are to its effects. If clinically indicated, additional testing to aid in diagnosis may be desired including but not limited to kidney and liver function test, X-rays and electrocardiograms.

Q: When is it ordered? 

A:A heavy metals panel may be ordered when you have signs and symptoms and/or your healthcare practitioner suspects that you have been acutely or chronically exposed to one or more heavy metals. It may be ordered periodically when you are treated for heavy metal poisoning.

Signs and symptoms of heavy metal exposure will vary in type and severity depending on the type and quantity of metal involved. Early symptoms of poisoning can be missed because they are often non-specific. Excessive exposure and damage to several different organs can occur even if you have no, few, or nonspecific symptoms. Some signs and symptoms of metal poisoning may include:

  • Abdominal pain, nausea, vomiting, and diarrhea
  • Nervous system symptoms such as numbness, tingling of hands and feet, weakness
  • Anemia
  • Kidney damage
  • Liver damage
  • In the lungs – irritation, fluid accumulation (edema) 
  • Brain dysfunction, memory loss
  • Mees lines (horizontal lines on nails)
  • Changes in behavior
  • Malformed bones in children, weakened bones
  • Developmental delays in children
  • In pregnant women – miscarriage, premature labor

You may also be monitored periodically when you may be exposed to metals in the workplace. Frequency of monitoring will depend on level of initial exposure, presence or absence of ongoing exposure, and current intervention. Safety measures minimize risk to employees and help address problems when they are identified. 

Q: How is someone exposed to toxic metals? 

A:Heavy metals can be absorbed through the skin, inhaled, or by eating or drinking contaminated food or liquids. You can be poisoned when the metals displace the essential elements in the body and begin to affect the normal function of various organs. Most people will never be sufficiently exposed to be harmed or require testing.

The majority of short-term and long-term exposures occur in the workplace, especially in industries that use metals to manufacture products; such as the cadmium, lead, and mercury used in batteries and the arsenic used in some pesticides. Exposures can also occur in agricultural workers, in people whose job it is to clean up contaminated environmental sites, in those who work with certain products such as auto mechanics working with car batteries, and in those with hobbies that involve the use of metals such as the lead used by stained glass artisans.

Pregnant women at risk of metal toxicity such as lead can pass metals to their developing babies (fetuses). This is because lead is able to cross the placenta. Also, lead can be passed to infants through breast milk. When mothers should and should not to breastfeed will depend on blood levels

Most exposures to excessive concentrations in the general population are primarily due to increased levels of metals in food or water, products that they use, or soil contamination in or near the areas that they work and live.

Related Words/Test:

Not Applicable

 

Test:Tissue Crossmatch B-celL (BY APPOINTMENT PER PATIENT)
Other Test Request Name:

Not Applicable

Test Composition:

Not Applicable

Intended Use:Not Applicable
Methodology:

Complement Dependent Cytotoxicity

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:
  • No patient preparation necessary.
  • Inform Special Test Section 1 day before the scheduled sample collection.
  • For recipients undergoing dialysis, draw blood before dialysis or at least 6 hours post dialysis
  • If the recipient has had a sensitizing event such as blood transfusion, pregnancy, etc., a blood specimen must be obtained at least 14 days after the sensitizing event.
  • Need Blood typing of Donor or Recipien
  • Call Special Test Section (By Appointment/Schedule.

NOTE: Do not collect specimen without approval of Special Test Section

 

Collection/Sample Container:

DONOR : 2 ACD tube and 1 Red top 2 mL EDTA (Bld.Typing)

RECIPIENT : 2 ACD and 1 Red top 2 mL EDTA (Bld.Typing)

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:

20 mL of whole blood (2 pcs ACD tube), 2mL whole blood EDTA and 
5 mL serum (each)

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

24 Hours 

•   Refrigerated Temperature (2-8°C):

24 Hours 

•   Freezer Temperature (-20°C):

Not Applicable

Transport Temperature:

Transport specimen at 15°C ~25 °C (room temperature)

Rejection Criteria:
  • Exceeded sample stability requirement
  • Quantity not sufficient
  • Improperly labeled specimen
  • Improper collection tube used
  • Clotted Sample
  • Over-filled or Under-filled tube
Running Day:

Monday to Friday

Cut Off Time:

Monday to Friday, 7;00 AM

TAT/Releasing of Results:

3 days after cut off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Not Applicable

Limitations/Interferences:

None Specified.

Frequently Asked Questions (FAQs):

Not Applicable

Related Words/Test:

Transfusion medicine, Compatibility between donor and recipient

 

Test:Tissue Crossmatch T-cell (BY APPOINTMENT PER PATIENT)
Other Test Request Name:

Not Applicable

Test Composition:

Not Applicable

Intended Use:Not Applicable
Methodology:

Complement Dependent Cytotoxicity

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:
  • No patient preparation necessary.
  • For recipients undergoing dialysis, draw blood before dialysis or at least 6 hours post dialysis
  • If the recipient has had a sensitizing event such as blood transfusion, pregnancy, etc., a blood specimen must be obtained at least 14 days after the sensitizing event.
  • Need Blood typing of Donor or Recipient
  • Call Special Test Section (By Appointment/Schedule)

NOTE: Do not collect specimen without approval of Special Test Section

 

Collection/Sample Container:

DONOR : 2 ACD tube and 1 Red top  2 mL EDTA (Bld.Typing)

RECIPIENT : 2 ACD and 1 Red top  2 mL EDTA (Bld.Typing)

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:

20 mL of whole blood (2 pcs ACD tube) 2mL whole blood EDTA and 
5 mL serum (each)

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

24 Hours 

•   Refrigerated Temperature (2-8°C):

24 Hours 

•   Freezer Temperature (-20°C):

Not Applicable

Transport Temperature:

Transport specimen at 15°C ~25 °C (room temperature)

Rejection Criteria:
  • Exceeded sample stability requirement
  • Quantity not sufficient
  • Improperly labeled specimen
  • Improper collection tube used
  • Clotted Sample
  • Over-filled or Under-filled tube
Running Day:

Monday to Friday

Cut Off Time:

Monday to Friday, 7:00 AM
 

TAT/Releasing of Results:

3 days after cut-off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Not Applicable

Limitations/Interferences:

None Specified.

Frequently Asked Questions (FAQs):

Not Applicable

Related Words/Test:

Transfusion medicine, Compatibility between donor and recipient

 

Test:Tissue Myacin Heavy Chain
Other Test Request Name:Not applicable
Test Composition:Not applicable
Intended Use:Not Applicable
Methodology:Not Applicable
Laboratory Section:Special Test
Special Instructions/Patient Preparations:No patient preparation necessary.
Collection/Sample Container:Slide holders or mailers for microscope slides; FFPE tissue blocks can either be wrapped in paper or placed in a box.
Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:• Formalin-fixed paraffin-embedded (FFPE) tissue block
• 1 H&E stained slide representative of the submitted tissue block.
• Histopathology report for the submitted tissue block
Alternative Specimen and Volume Requirement:Not Applicable
Specimen Stability  
•   Room Temperature (15-25°C):Indefinite
•   Refrigerated Temperature (2-8°C):Not applicable
•   Freezer Temperature (-20°C):Not applicable
Transport Temperature:• Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.
• FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.
• Transport specimen at 15°C ~25 °C (room temperature)
Rejection Criteria:• Incomplete information in the Request Form
• Improper or insufficient labeling of tissue blocks or slides
• Inadequate tissue available on the tissue block
Running Day:Batch Running
Cut Off Time:4:00 PM
TAT/Releasing of Results:After 10 days (excluding Saturdays, Sundays and Holidays)
Reference Interval/Result Interpretation:Not Applicable
Limitations/Interferences:None specified.
Frequently Asked Questions (FAQs):Not Applicable
Related Words/Test:ATP-dependent motor proteins, muscle contraction

 

Test:TNF - Alpha
Other Test Request Name:

• Cachectin
• Tumor Neurosis Factor

Test Composition:

Not applicable

Intended Use:

A cytokine which is a key inflammatory mediator. It is secreted by monocytes and macrophages (TNF-alpha), and by activated T lymphocytes (TNF-beta). This test is useful in post-transplantation monitoring.

Methodology:

Enzyme Immunoassay (EIA)

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:

No patient preparation necessary.

Collection/Sample Container:

Red or Gold Tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:

3 mL Serum

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

6 Days

•   Refrigerated Temperature (2-8°C):

7 Days

•   Freezer Temperature (-20°C):

Not applicable

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:

• Hemolyzed specimen
• Markedly lipemic specimen
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used

Running Day:

Batch Running

Cut Off Time:

Friday 4:00 PM

TAT/Releasing of Results:

14 days after cut-off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Available upon request

Limitations/Interferences:

None specified.

Frequently Asked Questions (FAQs):

Not Applicable

Related Words/Test:

Not Applicable

 

Test:Toluene
Other Test Request Name:
  • Toluene Blood
  • Methylbenzene
  • Phenylmethane

 

Test Composition:

Not Applicable

Intended Use:

An aromatic hydrocarbon often used as a substitute for benzene. It is neurotoxic and contains impurities which can induce hepatic and renal toxicity. Toluene is metabolically converted into hippuric acid.

Methodology:

Headspace Gas Chromatography (GC)

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:

No patient preparation required.

 

Collection/Sample Container:

Gray tube

 

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:

4mL Whole Blood

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

5 Days

•   Refrigerated Temperature (2-8°C):

14 Days

•   Freezer Temperature (-20°C):

30 Days

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:
  • Insufficient volume
  • Improperly labeled sample
  • Hemolyzed sample
  • Exceeded sample stability requirement

 

 

Running Day:

Batch Running

Cut Off Time:

Monday, 12:00 PM

 

TAT/Releasing of Results:

2 weeks after cut off (excluding Saturday, Sunday and Holidays)

 

Reference Interval/Result Interpretation:

Available Upon Request

 

Limitations/Interferences:

None specified.

Frequently Asked Questions (FAQs):

Q:How does toluene affect the body?

A:Symptoms worsen as exposure increases, and long term exposure may lead to tiredness, slow reaction, difficulty sleeping, numbness in the hands or feet, or female reproductive system damage and pregnancy loss. If swallowed,toluenecan cause liver and kidney damage.

Q:What does Toluene do to the brain?

A:Long-term and intense exposure totoluenevapors in humans who abuse spray paint and related substances has led to the recognition thattoluenehas a severe impact on central nervous system myelin. Chronictolueneabuse produces a devastating neurological disorder, of which dementia is the most disabling component.

Q:Can toluene cause birth defects?

A:Some mothers who breathed large amounts oftolueneduring pregnancy to get  high have had children withbirth defects, including retardation of mental abilities and growth.

Related Words/Test:

Tolstoy, Leo,Toltec,tolu,toluate,Toluca,Toluic,Toluic Acid,Toluidine,Toluidine Blue  O,Toluol

 

 

Test:Topiramate
Other Test Request Name:• Epitomax
• Topamax
Test Composition:Not applicable
Intended Use:Topiramate is an anticonvulsant used as an adjunctive treatment of partial-onset epilepsy. Therapeutic drug monitoring is useful to optimize dose and avoid toxicity.
Methodology:Enzyme Immunoassay (EIA)
Laboratory Section:Special Test
Special Instructions/Patient Preparations:• No patient preparation necessary.
• Note date and time of sample collection
• Note date and time of last intake of Topiramate medication
• Note dosage regimen and frequency of administration
• Note height and weight
Collection/Sample Container:Red tube (DO NOT USE GOLD TUBE or any other type of tube with gel separator)
Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:3 mL Serum
Alternative Specimen and Volume Requirement:Not Applicable
Specimen Stability  
•   Room Temperature (15-25°C):7 Days
•   Refrigerated Temperature (2-8°C):14 Days
•   Freezer Temperature (-20°C):30 Days
Transport Temperature:Transport specimen at 2 – 8 °C (with cold packs)
Rejection Criteria:• Hemolyzed specimen
• Markedly lipemic specimen
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used
Running Day:Batch Running
Cut Off Time:Friday 4:00 PM
TAT/Releasing of Results:14 days after cut-off (excluding Saturdays, Sundays and Holidays)
Reference Interval/Result Interpretation:Available upon request
Limitations/Interferences:None specified.
Frequently Asked Questions (FAQs):Not Applicable
Related Words/Test:Anticonvulsant (antiepilepsy) drug

 

Test:ToRCH (IgG/IgM)
Other Test Request Name:
  • ToRCH (IgG/IgM) Panel
  • ToRCH Quantitative

 

Test Composition:
  1. Toxoplasma IgG
  2. Toxoplasma IgM
  3. Rubella IgG
  4. Rubella IgM
  5. CMV IgG
  6. CMV IgM
  7. Herpes Simplex Virus 1&2 IgG
  8. Herpes Simplex Virus 1&2 IgM

Individual Test Codes

 

ToRCH9:

 

ü  TOXG93  – Toxoplasma IgG

ü  TOXOM9 – Toxoplasma IgM

ü  RUBG93  – Rubella IgG (CMIA)

ü  RUBM93  – Rubella IgM

ü  CMVG93 – CMV IgG(CMIA)

ü  CMVM93 – CMV IgM (CMIA)

ü  HSVG9     – HSV 1/2 IgG (CLIA)

ü  HSVM9    – HSV 1/2 IgM (CLIA)                                                                         

 

Note:

HSV Type 1 or 2 for both IgG and IgMCANNOTbe requested separately. You need to use codeHSVG9for combined HSV Type 1/2 IgG test or use codeHSVM9for HSV 1/2 IgM test. 

Intended Use:

ToRCH stands for 4 different infections that can adversely affect pregnant women and fetus and newborn children.

Used for the detection of IgG and IgM antibodies to Toxoplasma gondii, Rubella, Cytomegalovirus (CMV), and Herpes Simplex Virus 1&2 (HSV)

Methodology:

Special Test :

  • Toxoplasma IgM  - Immunoassay (IA)
  • HSV 1/2 IgG - Flow Immunofluorimetry
  • HSV 1/2 IgM – Enzyme-linked Immunosorbent Assay (ELISA)

Immunology (Chemiluminescent Microparticle Immunoassay (CMIA)):

  • Rubella IgG
  • Rubella IgM
  • CMV IgG and CMV IgM
  • Toxoplasma IgG
Laboratory Section:

Special Test :

  • Toxoplasma IgM
  • HSV 1/2 IgG
  • HSV 1/2 IgM

Immunology:

  • Rubella IgG /Rubella IgM
  • CMV IgG and CMV IgM
  • Toxoplasma IgG 
Special Instructions/Patient Preparations:
  • No patient preparation necessary.
  • Take note of patient’s medical history, clinical diagnosis and medications currently taken, if any.
Collection/Sample Container:

(4) pieces GOLD/RED TOP

     Note:3 pieces for Special Section
                1 piece for Immunology Section

 

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:

 

Special Test:8 mL Serum

Immunology2-4 mL Serum

 

Alternative Specimen and Volume Requirement:

Special Test:Not Applicable

 

Immunology:

  • Toxoplasma IgG – Plasma (Li- or Na- heparin, K EDTA, Na Citrate)
  • Rubella IgG – Plasma (K-EDTA, Li- or Na- heparin)
  • Rubella IgM – Plasma (K-EDTA, Li- or Na- heparin, Na Citrate)
  • CMV IgG – Plasma (Li- or Na- heparin, K- EDTA, Na- citrate, ACD, CPDA-1, CPD Potassium oxalate/sodium fluoride)
  • CMV IgM – Plasma (Li- or Na- heparin, K- EDTA, Na citrate)
Specimen Stability  
•   Room Temperature (15-25°C):

Special test :

Toxoplasma IgM: 4 days

HSV 1/2 IgG: Not applicable

HSV 1/2 IgM: Not Applicable

 

Immunology:

Toxoplasma IgG: 3 Days

Rubella IgG: Not specified

Rubella IgM: Not specified

CMV IgG & CMV IgM: Not specified

 

•   Refrigerated Temperature (2-8°C):

Special test :

Toxoplasma IgM: 7 days

HSV 1/2 IgG : 7 days

HSV 1/2 IgM: 5 days

 

Immunology:

Toxoplasma IgG: 14 days

Rubella IgG/ Rubella IgM: 14 days

CMV IgG & CMV IgM: 14 days

 

•   Freezer Temperature (-20°C):

Special test :

Toxoplasma IgM: 28 days

HSV 1/2 IgG: Not Applicable

HSV 1/2 IgM: Not Applicable

  • Room Temperature (150C -250C): Not Applicable
  • Refrigerated Temperature (2°C to 8°C) : 5 days
  • Freezer Temperature (-20°C) : Not Applicable

 

 

 

Immunology:

Toxoplasma IgG: Not specified

Rubella IgG/ Rubella IgM: 1 month

CMV IgG & CMV IgM: >14 days (Not specified)

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:
  • Improperly labeled specimens
  • Hemolyzed specimens
  • Lipemic specimens
  • Quantity not sufficient
  • Specimen storage and transport exceeded required temperature
Running Day:

Special Test :

  • Batch Running: Toxoplasma IgM; HSV 1/2 IgG
  • Monday up to Friday :  HSV 1/2 IgM

 

Immunology:

  • Monday up to Friday : Rubella IgG
  • Friday : CMV IgG; CMV IgM; Toxoplasma IgG, Rubella IgM

 

Cut Off Time:

Special Test :

  • Toxoplasma IgM – Monday, 12:00 PM
  •  HSV 1/2 IgM – Monday to Friday, 7:00 AM
  • HSV 1/2 IgG – Friday, 12:00 PM

 

Immunology:

6:00 PM

TAT/Releasing of Results:

Special Test:

Toxoplasma IgM; HSV 1/2 IgG

  • 2 weeks after cut-off (excluding Saturdays, Sundays and Holidays)

 HSV 1/2 IgM

  • 3 days after cut-off (excluding Saturdays, Sundays and Holidays)

 

Immunology:

Toxoplasma IgG, Rubella IgG, CMV IgG & CMV IgM

  • ROUTINE(on running days): 4 hours after receipt of specimen/ arrival of messenger
  • STAT(on running days only): 2½ hours after receipt of specimen/ arrival of messenger

 

Reference Interval/Result Interpretation:

Special Test :Available upon request

Immunology :

  • Toxoplasma IgG

NONREACTIVE: <1.60 IU/mL

  • A nonreactive result does not exclude the possibility of Toxoplasma gondii infection. If early disease is suspected, suggest retest in 3 weeks.

EQUIVOCAL: 1.60~2.99 IU/mL

  • Grayzone, may contain low levels of IgG. Suggest Toxoplasma IgM test or repeat Toxoplasma IgG in 2 to 3 weeks.

REACTIVE: >/= 3.00 IU/mL

  • May indicate past or acute infection.
  • Rubella IgG:

       NEGATIVE:       <5.00 IU/mL

       EQUIVOCAL:      5.00~9.99 IU/mL

POSITIVE: >/= 10.0 IU/mL

  • Rubella IgM:

       NEGATIVE:       <1.20  S/CO

       EQUIVOCAL:      1.20 ~ 1.59 S/CO

       POSITIVE: >/= 1.60 S/CO

  • CMV IgG:

       NEGATIVE:      <6.00 AU/mL

POSITIVE: >/=   6.00 AU/mL

  • CMV IgM:

NEGATIVE: <0.85 Index

       EQUIVOCAL: 0.85~0.99 Index

POSITIVE: >/= 1.00 Index

 

Limitations/Interferences:

Special Test:Not Applicable

 

Immunology:

 

Toxoplasma IgG:None indicated

 

Rubella IgG/ Rubella IgM :Heterophilic antibodies, Mouse monoclonal antibodies and human anti-mouse antibodies (HAMA)

 

CMV IgG:

• Heterophilic antibodies in human serum can react with reagent immunoglobulins, interfering with in vitro immunoassays. Patients routinely exposed to animals or to animal serum products can be prone to this interference, and anomalous values may be observed.
• Samples from patients who have received preparations of mouse monoclonal antibodies for diagnosis or therapy may contain human anti-mouse antibodies (HAMA). Samples containing HAMA may produce anomalous values when tested with assay kits that employ mouse monoclonal antibodies.

 

CMV IgM:

·         IgM rheumatoid factor (RF) in combination with CMV specific IgG can lead to false reactive results in IgM detecting assays.

·         Samples from patients who have received preparations of mouse monoclonal antibodies for diagnosis or therapy may contain human anti-mouse antibodies (HAMA). Specimens containing HAMA may produce anomalous values when tested with assay kits that employ mouse monoclonal antibodies.

Heterophilic antibodies in human serum can react with reagent immunoglobulins, interfering with in vitro immunoassays.

Frequently Asked Questions (FAQs):

Q: When is TORCH test ordered?

A:The TORCH panel may be ordered when an infant shows any of the following signs/symptoms:

This panel may sometimes be ordered to a woman before becoming pregnant or very early in thepregnancy (first trimester).

 

Related Words/Test:

Rubella Test, Parotitis, Measles Blood Test; Mumps Blood Test, Measles Viral Culture, Measles by RT-PCR, Mumps Viral Culture, Mumps by RT-PCR

 

 

Test:Total Acid Phosphatase
Other Test Request Name:

• ACP

Test Composition:

Not applicable

Intended Use:

• Acid phosphatase is an enzyme produced in the prostate gland, semen, liver, spleen, blood cells, and bone marrow. If these organs and tissues are not functioning right, they may release acid phosphatase into the bloodstream.
• Acid phosphatase testing is done to diagnose whether prostate cancer has spread to other parts of the body (metastasized), and to check the effectiveness of treatment. The test has been largely supplanted by the prostate specific antigen test (PSA).

Methodology:

Colorimetric

Laboratory Section:

Chemistry

Special Instructions/Patient Preparations:
  • Blood collection should be done at least 3 days after rectal examination, prostatic massage, or urinary catheterization.
  • If sample cannot be sent to HPD Main Laboratory within8 hoursof collection, request for stabilizing reagent from HPD Main Laboratory prior to sample collection to extend sample stability.
  • Stabilizing Reagent Preparation: Bottle 3 of reagent pack. Add 1 drop (30uL) of stabilizing reagent to 1 mL of serum.
  • Separate serum from red cells immediately. Transfer 1mL of serum to tube with stabilizing reagent.
    NOTE: Upon addition of stabilizing reagent. Sample cannot be used for other analysis anymore.
Collection/Sample Container:

Red or Gold tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:

1-3 mL Serum

Alternative Specimen and Volume Requirement:

Not applicable

Specimen Stability  
•   Room Temperature (15-25°C):
  • 8 Days (With Stabilizing Reagent)
  • 2 Hours (Without Stabilizing Reagent)
•   Refrigerated Temperature (2-8°C):
  • 8 Days (With Stabilizing Reagent)
  • 8 Hours (Without Stabilizing Reagent)
•   Freezer Temperature (-20°C):
  • 4 Months (With Stabilizing Reagent)
  • 1 Day (Without Stabilizing Reagent)
Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:

• Hemolyzed specimen
• Markedly lipemic specimen
• Exceeded sample stability and temperature requirement
• Quantity not sufficient
• Improperly labeled specimens
• Plasma specimens

Running Day:

Every 3rd week of the month

Cut Off Time:

6 PM
 

TAT/Releasing of Results:

ROUTINE (on running days)
• 4 hours after receipt of specimen/ arrival of messenger
STAT (on running day only)
• After 2½ HOURS from extraction/messenger arrival

Reference Interval/Result Interpretation:

Prostatic ACP:

Total Acid Phosphatase:

MALE:

0.00-0.110 ukat/L (0.00-6.60 U/L)

FEMALE:

0.00-0.109 ukat/L (0.00-6.50 U/L)

Limitations/Interferences:

• Cefoxitine and doxycycline cause artificially high non-prostatic
acid phosphatase results.
• In very rare cases, gammopathy, in particular type IgM (Waldenstrom’s macroglobulinemia), may cause unreliable results.
• The addition of stabilizer to the sample interferes with the determination of other parameters.

Frequently Asked Questions (FAQs):

Not applicable

Related Words/Test:

Prostate Specific Antigen, PSA, Prostatic Acid Phosphatase

 

Test:Total ACP & Prostatic ACP
Other Test Request Name:

• Prostatic Acid Phosphatase
• PAP
• Prostatic Specific Acid Phosphatase
• PSAP

Test Composition:

1. Total Acid Phosphatase
2. Prostatic ACP

Intended Use:

• More helpful for diagnosis in advanced prostate cancer than in early prostate cancer.
• Acid phosphatase testing is done to diagnose whether prostate cancer has spread to other parts of the body (metastasized), and to check the effectiveness of treatment. The test has been largely supplanted by the prostate specific antigen test (PSA).

Methodology:

Colorimetric

Laboratory Section:

Chemistry

Special Instructions/Patient Preparations:

    • Blood collection should be done at least 3 days after rectal examination, prostatic massage, or urinary catheterization.
    • If sample cannot be sent to HPD Main Laboratory within 8 hours of collection, request for stabilizing reagent from HPD Main Laboratory prior to sample collection to extend sample stability.
    • Stabilizing Reagent Preparation: Bottle 3 of reagent pack. Add 1 drop (30uL) of stabilizing reagent to 1 mL of serum.
    • Separate serum from red cells immediately. Transfer 1mL of serum to tube with stabilizing reagent.
    NOTE: Upon addition of stabilizing reagent. Sample cannot be used for other analysis anymore.

Collection/Sample Container:

Red or Gold tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:

1-3 mL Serum

Alternative Specimen and Volume Requirement:

Not applicable

Specimen Stability  
•   Room Temperature (15-25°C):
  • 8 Days (With Stabilizing Reagent)
  • 2 Hours (Without Stabilizing Reagent)
•   Refrigerated Temperature (2-8°C):
  • 8 Days (With Stabilizing Reagent)
  • 8 Hours (Without Stabilizing Reagent)
•   Freezer Temperature (-20°C):
  • 4 Months (With Stabilizing Reagent)
  • 1 Day (Without Stabilizing Reagent)
Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:

• Hemolyzed specimen
• Markedly lipemic specimen
• Exceeded sample stability and temperature requirement
• Quantity not sufficient
• Improperly labeled specimens
• Plasma specimens

Running Day:

Every 3rd week of the month

Cut Off Time:

6:00 PM

TAT/Releasing of Results:

ROUTINE (on running days)
• 4 hours after receipt of specimen/ arrival of messenger
STAT (on running day only)
• After 2½ HOURS from extraction/messenger arrival

Reference Interval/Result Interpretation:

Prostatic ACP:

0.00-0.058 ukat/L (0.00-3.50 U/L)

Total Acid Phosphatase:

MALE:

0.00-0.110 ukat/L (0.00-6.60 U/L)

FEMALE:

0.00-0.109 ukat/L (0.00-6.50 U/L)

Limitations/Interferences:

• Cefoxitine and doxycycline cause artificially high non-prostatic
acid phosphatase results.
• In very rare cases, gammopathy, in particular type IgM (Waldenstrom’s macroglobulinemia), may cause unreliable results.
• The addition of stabilizer to the sample interferes with the determination of other parameters.

Frequently Asked Questions (FAQs):

Not applicable

Related Words/Test:

Prostate Specific Antigen, PSA, Prostatic Acid Phosphatase

 

Test:Total IgE (ECLIA)
Other Test Request Name:

• Immunoglobulin E
• Quantitative IgE

Test Composition:

Not applicable

Intended Use:

Determination of total IgE is useful as an aid in the diagnosis of allergic diseases

Methodology:

Electrochemiluminescence Immunoassay (ECLIA)

Laboratory Section:

Immunology

Special Instructions/Patient Preparations:

Samples should not be taken from patients receiving therapy with high biotin doses (i.e. > 5 mg/day) until at least 8 hours following the last biotin administration.

Collection/Sample Container:

Red or Gold tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:

1-3 mL Serum

Alternative Specimen and Volume Requirement:

1-3 mL Plasma (Li-, Na-heparin, K3-EDTA)

Specimen Stability  
•   Room Temperature (15-25°C):

Not specified

•   Refrigerated Temperature (2-8°C):

7 Days

•   Freezer Temperature (-20°C):

6 Months (Sample may be frozen 5 times)

Transport Temperature:

Transport specimen at 2°C – 8 °C (with cold packs)

Rejection Criteria:

• Hemolyzed specimen
• Markedly lipemic specimen
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used

Running Day:

Monday and Thursday

Cut Off Time:

6:00 pm

TAT/Releasing of Results:

ROUTINE (on running days)
• 4 hours after receipt of specimen/ arrival of messenger
STAT (on running days only)
• 2½ hours from extraction/messenger arrival

Reference Interval/Result Interpretation:

Adult: <100 IU/mL
Neonate: <1.5 IU/mL
Children:
1 y/o: <15 IU/mL
2-5 y/o: <60 IU/mL
6-9 y/o: <90 IU/mL
10-15 y/o: <200 IU/mL

Limitations/Interferences:

• Samples should not be taken from patients receiving therapy with high biotin doses (i.e. > 5 mg/day) until at least 8 hours following the last biotin administration.
• In rare cases, interference due to extremely high titers of antibodies to analyte-specific antibodies, streptavidin or ruthenium can occur.
• An interference was found for samples from patients treated with Xolair (omalizumab). Do not use samples from patients under treatment with Xolair (omalizumab) or similar drugs containing anti-IgE antibodies.

Frequently Asked Questions (FAQs):

Not applicable

Related Words/Test:

Allergy Blood Testing, Quantitative Immunoglobulins, Protein Electrophoresis, Eosinophil Count

 

Test:Total Protein/ALB/GLOB
Other Test Request Name:

• Albumin/Globulin Ratio
• A/G Ratio
• Albumin to Globulin Ratio

Test Composition:

1. Total Protein
2. Albumin
3. Globulin
4. Albumin/ Globulin Ratio

Intended Use:

• May be used in a variety of settings to help diagnose disease, to monitor changes in health status with treatment or with disease progression, and as a screen that may indicate the need for other kinds of testing.

Methodology:

Colorimetric

Laboratory Section:

Chemistry

Special Instructions/Patient Preparations:

• No patient preparation necessary.

Collection/Sample Container:

Red or Gold tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:

1-3 mL Serum

Alternative Specimen and Volume Requirement:

1-3 mL Plasma (Li- or Na-Heparin)

Specimen Stability  
•   Room Temperature (15-25°C):

4 Hours

•   Refrigerated Temperature (2-8°C):

3 Days

•   Freezer Temperature (-20°C):

6 Months

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:

• Improper collection tube used
• Specimen stored/transported outside the temperature range
• Quantity not sufficient
• Hemolyzed specimen
• Lipemic specimen
• Improperly labeled specimen
• EDTA plasma

Running Day:

Daily

Cut Off Time:

Monday to Saturday:6:00 PM

 

TAT/Releasing of Results:

RUNNING TIME: 6:00 AM onwards

RELEASING TIME: ROUTINE
• 4 hours after receipt of specimen/ arrival of messenger
RELEASING TIME: STAT
• After 2½ hours from extraction/messenger arrival

Reference Interval/Result Interpretation:

TOTAL PROTEIN:
0-3Y: 59.00-70.00 g/L (5.90-7.00 g/dL)
3Y & 1 Day - 6Y: 59.00-78.00 g/L (5.90-7.80 g/dL)
6Y & 1 Day - 9Y: 62.00-81.00 g/L (6.20-8.10 g/dL)
9Y & 1 Day - 11Y : 63.00-86.00 g/L (6.30-8.60 g/dL)
11Y & 1 Day - 999Y: 64.00-87.00 g/L (6.40-8.70 g/dL)

ALBUMIN:
0-3Y: 34.00-42.00 g/L (3.40-4.20 g/dL)
3Y & 1 Day - 6Y: 35.00-52.00 g/L (3.50-5.20 g/dL)
6Y & 1 Day - 11Y: 37.00-56.00 g/L (3.70-5.60 g/dL)
11Y & 1 Day - 999Y: 35.00-50.00 g/L (3.50-5.00 g/dL)

GLOBULIN: 23.00-35.00 g/L (2.50-3.50 g/dL)

Limitations/Interferences:

• Substances that may interfere with the assay: Hemoglobin, Bilirubin
• Albumin concentrations vary with posture. Results from an upright posture may be approximately 0.3 g/dL (3 g/L) higher than those from a recumbent posture.

Frequently Asked Questions (FAQs):

Not applicable

Related Words/Test:

Albumin, Liver Panel, Protein Electrophoresis, Comprehensive Metabolic Panel, BUN, Creatinine, Urine Protein, Body Fluid Analysis

 

Test:Toxocara Ab(Confirmatory)
Other Test Request Name:
  • Toxocara canis
  • VLM - serology
  • Visceral larva migrans - serology
  • Toxocara Western blot IgG
Test Composition:

Not applicable

Intended Use:

Humans can become accidentally infected with the larvae of certain nematode worms whose natural host is either the dog (T. canis) or the cat (T. catis). This leads to larva migrans which presents with severe eosinophilia. Serological analysis is important in the diagnosis of this condition despite the fact that there is extensive cross-reactivity (which can be confirmed by Western blot analysis).

Methodology:

Westernblot

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:

No patient preparation necessary.

Collection/Sample Container:

Red or Gold Tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
:

3 mL Serum

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

7 Days

•   Refrigerated Temperature (2-8°C):

14 Days

•   Freezer Temperature (-20°C):

30 Days

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:
  • Hemolyzed specimen
  • Markedly lipemic specimen
  • Exceeded sample stability requirement
  • Quantity not sufficient
  • Improperly labeled specimen
  • Improper collection tube used
Running Day:

Batch Running

Cut Off Time:

Friday 4:00 PM

TAT/Releasing of Results:

2 weeks after cut-off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Available upon request

Limitations/Interferences:

None specified.

Frequently Asked Questions (FAQs):

Not Applicable

Related Words/Test:

Toxocariasis, visceral larva migrans (VLM)