Laboratory Test Preparation
Test
24H Urine Calcium
Other Test Request Name
• 24 Hour Urine Ca
Test Composition
Not applicable
Intended Use
• Used for evaluating whether high calcium levels in the urine resulted in the development of a kidney stone
• Used for evaluating whether your dietary intake of calcium is high enough
• Used for evaluating how well your intestines are absorbing the calcium
• Used for detecting conditions that lead to calcium loss from your bones
• Used for evaluating how well your kidney is functioning
• Used when looking for problems with the parathyroid gland
Methodology
Colorimetric
Laboratory Section
Chemistry
Special Instructions/patient Preparations
24 Hour Urine Collection procedure:
1. Void at the beginning of the collection period and discard the specimen.
2. Collect all urine including the final specimen voided at the end of the collection period.
3. Keep specimens on ice during collection.
4. Label the bottle with patient name and date & time collection started and ended.
Collection/Sample Container
Clean plastic container
Specimen and Volume Requirement Note:Follow tube manufacturer recommendation.
24 Hr Urine Sample
NOTE: 60mL aliquot of the whole 24hr urine sample is acceptable. Ensure correct aliquoting procedure is followed (place all samples in one container and mix well prior to obtaining an aliquot)
Alternative Specimen and Volume Requirement
Not applicable
Specimen Stability
• Room Temperature (15-25°C)
5 Days
• Refrigerated Temperature (2-8°C)
5 Weeks
• Freezer Temperature (-20°C)
6 Months
Transport Temperature
Transport specimen at 2 – 8 °C (with cold packs)
Rejection Criteria
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimens
• Improper urine collection
Running Day
Daily
Cut Off Time
Monday to Saturday:10:00 PM
Sunday:6:00PM
TAT/Releasing of Results
RELEASING: 6:00 am onwards
ROUTINE
• 4 hours after receipt of specimen/ arrival of messenger
STAT
• 2½ hours from extraction/messenger arrival
Reference Interval/Result Interpretation
2.50-7.50 mmol/24Hr (100.00-300.00 mg/24Hr)
Limitations/Interferences
None specified
Frequently Asked Questions (FAQs)
Not applicable
Related Words/Test
hypocalcemia, hypercalcemia
Test
24H Urine Calcium
Other Test Request Name
• 24 Hour Urine Ca
Test Composition
Not applicable
Intended Use
• Used for evaluating whether high calcium levels in the urine resulted in the development of a kidney stone
• Used for evaluating whether your dietary intake of calcium is high enough
• Used for evaluating how well your intestines are absorbing the calcium
• Used for detecting conditions that lead to calcium loss from your bones
• Used for evaluating how well your kidney is functioning
• Used when looking for problems with the parathyroid gland
Methodology
Colorimetric
Laboratory Section
Chemistry
Special Instructions/Patient Preparations
24 Hour Urine Collection procedure:
1. Void at the beginning of the collection period and discard the specimen.
2. Collect all urine including the final specimen voided at the end of the collection period.
3. Keep specimens on ice during collection.
4. Label the bottle with patient name and date & time collection started and ended.
Collection/Sample Container
Clean plastic container
Specimen and Volume RequirementNote:Follow tube manufacturer recommendation.
24 Hr Urine Sample
NOTE: 60mL aliquot of the whole 24hr urine sample is acceptable. Ensure correct aliquoting procedure is followed (place all samples in one container and mix well prior to obtaining an aliquot)
Alternative Specimen and Volume Requirement
Not applicable
Specimen Stability
• Room Temperature (15-25°C)
5 Days
• Refrigerated Temperature (2-8°C)
5 Weeks
• Freezer Temperature (-20°C)
6 Months
Transport Temperature
Transport specimen at 2 – 8 °C (with cold packs)
Rejection Criteria
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimens
• Improper urine collection
Running Day
Daily
Cut Off Time
Monday to Saturday:10:00 PM
Sunday:6:00PM
TAT/Releasing of Results
RELEASING: 6:00 am onwards
ROUTINE
• 4 hours after receipt of specimen/ arrival of messenger
STAT
• 2½ hours from extraction/messenger arrival
Reference Interval/Result Interpretation
2.50-7.50 mmol/24Hr (100.00-300.00 mg/24Hr)
Limitations/Interferences
None specified
Frequently Asked Questions (FAQs)
Not applicable
Related Words/Test
hypocalcemia, hypercalcemia
Test
24Hr Protein/Crea Ratio
Other Test Request Name
Urine Protein to Creatinine Ratio
Test Composition
• 24-Hour Urine Protein
• 24-Hour Urine Creatinine
• 24-Hour Urine Protein/Creatinine Ratio
Intended Use
• Used to monitor a person with known kidney disease or damage.
• May be used to screen people on a regular basis when they are taking a medication that may affect their kidney function.
Methodology
Urine Protein: Turbidimetric
Urine Creatinine: Enzymatic
Laboratory Section
Chemistry
Special Instructions/Patient Preparations
1. Refrain from eating meat and doing strenuous physical activity 8-12 hours before sample collection.
2. Refrain from taking medicines such as Acetaminophen (Paracetamol), N-acetyl-p-benzoquinone imine (NAPQI) metabolites, N-Acteylcysteine (NAC), Metamizole (Novaminsulfone, Dipyrone), 4-aminoantipyrine (4-AAP) and 4-Methylamino-antipyrine (4-MAP) metabolites 8-12 hrs before sample collection.
Note: If recommended preparation is not followed, you may accept but NOTE in clinical info.
24 Hour Urine Collection procedure:
1. Void at the beginning of the collection period and discard the specimen.
2. Collect all urine including the final specimen voided at the end of the collection period.
3. Keep specimen on ice during collection.
4. Label the bottle with patient name and date & time collection started and ended.
Collection/Sample Container
Clean plastic container
Specimen and Volume RequirementNote: Follow tube manufacturer recommendation.
• 24 Hr Urine Sample
NOTE: 60mL aliquot of the whole 24hr urine sample is acceptable. Ensure correct aliquoting procedure is followed (place all samples in one container and mix well prior to obtaining an aliquot)
Alternative Specimen and Volume Requirement
Not applicable
Specimen Stability
• Room Temperature (15-25°C)
1 Day
• Refrigerated Temperature (2-8°C)
6 Days
• Freezer Temperature (-20°C)
1 Month
Transport Temperature
Transport specimen at 2°C – 8 °C (with cold packs)
Rejection Criteria
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimens
• Improper urine collection
Running Day
Daily
Cut Off Time
Monday to Saturday:6:00 PM
TAT/Releasing of Results
RUNNING TIME: 11:00AM onwards
ROUTINE
• 4 hours after receipt of specimen/ arrival of messenger
STAT
• After 2½ HOURS from extraction/messenger arrival
Reference Interval/Result Interpretation
24 hour urine Creatinine
Females: 6-13 mmol/24h (0.68-1.47 g/24h)
Males: 9-19 mmol/24h (1.02-2.15 g/24h)
24 Hour Urine Protein: 0.00-0.14 g/24H (0.00-140.00 mg/24H)
*No established range for 24Hr Urine Protein/Creatinine Ratio
Reference Interval/Result Interpretation
24 hour urine Creatinine
Females: 6-13 mmol/24h (0.68-1.47 g/24h)
Males: 9-19 mmol/24h (1.02-2.15 g/24h)
24 Hour Urine Protein: 0.00-0.14 g/24H (0.00-140.00 mg/24H)
*No established range for 24Hr Urine Protein/Creatinine Ratio
Limitations/Interferences
• Calcium dobesilate (e.g. Dexium), Levodopa and alpha methyldopa cause artificially low creatinine results. Dicynone (Etamsylate) at therapeutic concentrations may lead to falsely low results.
• High homogentisic acid concentrations in urine samples lead to false results.
• Acetaminophen, Acetylcysteine and Metamizole are metabolized quickly. Therefore, interference from these substances is unlikely but cannot be excluded.
• Levodopa, methyldopa and Na2 cefoxitin cause artificially high total protein results and calcium dobesilate causes artificially low protein results.
Frequently Asked Questions (FAQs)
Not applicable
Related Words/Test
Kidney Function, Urinalysis, Albumin, Urine Albumin, Protein Electrophoresis, Total Protein, BUN, Creatinine, eGFR
Test
ACE (CSF)
Other Test Request Name
Angiotensin Converting Enzyme
dipeptidyl carboxypeptidase I
peptidyl dipeptidase
carboxycathepsin
hypertensin converting enzyme peptidyl dipeptidase I
Angioconvertase
Test Composition
Not applicable
Intended Use
This test is useful in diagnosing patients with sarcoidosis involving the central nervous system and meninges.
Methodology
Kinetic
Laboratory Section
Special Test
Special Instructions/Patient Preparations
No patient preparation necessary.
Collection/Sample Container
Sterile Container
Specimen and Volume Requirement
Note: Follow tube manufacturer recommendation.
2 mL Cerebrospinal Fluid (CSF)
Alternative Specimen and Volume Requirement
Not Applicable
Specimen Stability
• Room Temperature (15-25°C)
24 Hours
• Refrigerated Temperature (2-8°C)
7 Days
• Freezer Temperature (-20°C)
28 days
Transport Temperature
Transport specimen at 2 – 8 °C (with cold packs)
Rejection Criteria
Insufficient volume
Incorrect storage and transport temperature of specimen
Insufficient volume
Incorrect collection container used
Improperly labeled specimen
Running Day
Batch Running
Cut Off Time
Monday, 12:00 PM
TAT/Releasing of Results
2 weeks after cut-off (excluding Saturdays, Sundays and Holidays)
Reference Interval/Result Interpretation
Available upon request
Limitations/Interferences
None specified.
Frequently Asked Questions (FAQs)
Q:Why Get Tested?
A: To help diagnose and monitor sarcoidosis; to help differentiate this systemic condition from other disorders causing similar symptoms
Q: When To Get Tested?
A: When you have granulomas that produce small bumps under the skin, a lingering cough, red watery eyes, and/or other symptoms suggestive of sarcoidosis; regularly when you have active sarcoidosis to monitor its course
Related Words/Test
Serum Angiotensin Converting Enzyme; SACE, Angiotensin-Converting Enzyme, Acid-Fast Bacillus (AFB) Testing;Sputum Culture Bacterial, Liver Panel, Complete Blood Count, Calcium
Test
ACE LEVEL
Other Test Request Name
Angiotensin Converting Enzyme
dipeptidyl carboxypeptidase I
peptidyl dipeptidase
carboxycathepsin
hypertensin converting enzyme peptidyl dipeptidase I
Angioconvertase
ACE - Angiotensin Converting Enzyme - serum
Test Composition
Not Applicable
Intended Use
Peptidyl-dipeptidase A is a catalytic glycoprotein which cleaves angiotensin I to generate the active form of the hormone, angiotensin II. This test is of diagnostic and prognostic use in sarcoidosis and other lung pathologies such as silicosis and asbestosis (diffuse fibrous pneumoconiosis caused by inhaling asbestos fibers).
Methodology
Chemistry - Spectrophotometry
Laboratory Section
Special Test
Special Instructions/Patient Preparations
No patient preparation necessary.
Collection/Sample Container
Red or Gold tube
Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
3 mL Serum
Alternative Specimen and Volume Requirement
Not Applicable
Specimen Stability
• Room Temperature (15-25°C)
24 Hours
• Refrigerated Temperature (2-8°C)
7 Days
• Freezer Temperature (-20°C)
28 Days
Transport Temperature
Transport specimen at 2 – 8 °C (with cold packs)
Rejection Criteria
Hemolyzed specimen
Quantity Not sufficient
Improperly labeled specimen
Markedly lipemic sample
container used
Wrong
Running Day
Batch Running
Test
Acetylcholine Receptor Ab
Other Test Request Name
- Anti-Neuromuscular Junction Antibodies / RAC
- Anti-Cholinesterase antibodiesMyasthenia
- Myasthenia Gravis Antibody
- AChR Antibody
- Acetylcholine Receptor Binding Antibody
Test Composition
Not applicable
Intended Use
For serological diagnosis of myasthenia gravis
Methodology
Enzyme-Linked lmmunosorbent Assay (ELISA)
Laboratory Section
Serology
Special Instructions/Patient Preparations
No patient preparation necessary.
Collection/Sample Container
Red or Gold tube
Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
2 mL Serum
Alternative Specimen and Volume Requirement
2mL Plasma (EDTA, Heparin or Citrate)
Specimen Stability
• Room Temperature (15-25°C)
Not specified
• Refrigerated Temperature (2-8°C)
14 Days
• Freezer Temperature (-20°C)
Not specified
Transport Temperature
Transport specimen at 2 – 10 °C (with cold packs)
Rejection Criteria
- Lipemic specimens
- Hemolyzed specimens
- lcteric sam ples
- Specimens showing bacterial contamination
- lmproperly la beled specimens
- Quantity not sufficient
- Specimen stored exceeded outside requlred stability
Running Day
Batch Running every Thursday
Cut Off Time
Wednesday, 9:00 PM
TAT/Releasing of Results
Friday, 4:00PM
Reference Interval/Result Interpretation
Not applicable
Limitations/Interferences
None specified.
Frequently Asked Questions (FAQs)
Not applicable
Related Words/Test
Gravis; Muscle nicotinic Acetylcholine Receptor (AChR) Binding Antibody; AChR Antibody; Myasthenia Gravis Antibodies; Acetylcholine Receptor Binding Antibody; Acetylcholine Receptor Blocking Antibody; Acetylcholine Receptor Modulating Antibody
Test
ACTH (ECLIA)
Other Test Request Name
• Adenocorticotropic Hormone
• Corticotropin
• Cosyntropin
Test Composition
Not applicable
Intended Use
Usually used together with Cortisol for differential diagnosis of abnormal ACTH levels
- Plasma ACTH measurements are useful in the differential diagnosis of pituitary Cushing's disease (ACTH hypersecretion), autonomous ACTH producing pituitary tumors (e.g. Nelson's syndrome), hypopituitarism with ACTH deficiency and ectopic ACTH syndrome.
- ACTH determinations can be used together with functional or stimulation tests to diagnose the origin of glucocorticoid overproduction. Similarly, ACTH measurements can be employed to facilitate differential diagnosis of adrenocortical insufficiency (Addison’s disease).
Methodology
Electrochemiluminescence Immunoassay (ECLIA)
Laboratory Section
Immunology
Special Instructions/Patient Preparations
- No patient preparation required.
- Blood extraction should be done between 7:00 AM to 10:00 AM unless specified by requesting clinician.
- Use ice-cooled (chilled) EDTA tube.
- Note: Branches are required to store their EDTA Tubes at 2°C -8°C
Collection/Sample Container
CHILLED EDTA
Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
1-3 mL Plasma
Alternative Specimen and Volume Requirement
Not applicable
Specimen Stability
• Room Temperature (15-25°C)
2 Hours
• Refrigerated Temperature (2-8°C)
24 Hours
• Freezer Temperature (-20°C)
4 Weeks (Freeze only once)
Transport Temperature
• Whole blood/ Plasma specimens should be immediately placed on ice (2°C -8°C)
• Transport to laboratory within 2 hours after collection (for whole blood) or within 24 hours (for plasma specimens).
NOTE: It is very important that 2-8°C temperature is maintained during sample transport as ACTH test is highly affected by temperature storage.
Rejection Criteria
• Hemolyzed specimen
• Markedly lipemic specimen
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used
Running Day
Tuesday
Cut Off Time
Monday to Saturday:10:00 PM
Sunday:6:00PM
TAT/Releasing of Results
RUNNING TIME: 10:00 AM onwards
RELEASING TIME: ROUTINE
• 4 hours after receipt of specimen/ arrival of messenger
RELEASING TIME: STAT
• After 2½ hours from extraction/messenger arrival
Reference Interval/Result Interpretation
(7AM-10AM):7.20-63.30
Limitations/Interferences
•ACTH concentrations vary considerably depending on physiological conditions. Therefore, ACTH results should always be evaluated together with simultaneously measured cortisol.
Frequently Asked Questions (FAQs)
Not applicable
Related Words/Test
Cortisol, Androstenedione
Test
Activated Partial Thromboplastin Time (aPTT/ PTT)
Other Test Request Name
• Activated Partial thromboplastin Time (APTT)
• PTT
Test Composition
Not applicable
Intended Use
• Aids in the diagnosis of bleeding disorders
• Monitors response to heparin therapy
Methodology
Laboratory Section
Hematology
Special Instructions/Patient Preparations
• No patient preparation necessary.
• DO NOT use glass collection and transport tube.
Collection/Sample Container
Citrated Blue top (Plastic)
Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
1-2 mL Citrated Plasma
Alternative Specimen and Volume Requirement
Not applicable
Specimen Stability
• Room Temperature (15-25°C)
4 Hours
• Refrigerated Temperature (2-8°C)
Not applicable
• Freezer Temperature (-20°C)
2 weeks
Transport Temperature
• Sample must be sent to laboratory immediately after collection at 15-25°c
• Samples that cannot be sent to HPD laboratory within 4 hours should be stored at -20°C and should be transported with dry ice.
Rejection Criteria
• Specimen in glass satellite tube.
• Improperly labeled specimens
• Hemolyzed specimens
• Quantity not sufficient
• Specimen transported outside the required temperature
• Insufficient volume of blood to anticoagulant
Running Day
Daily
Cut Off Time
Monday to Saturday
• 6:00 PM
Sunday
• 3:00 PM
TAT/Releasing of Results
ROUTINE
• 3 hours after receipt of specimen/ arrival of messenger
STAT
• After 1½ HOURS from extraction/messenger arrival
Reference Interval/Result Interpretation
25.00-40.00 Seconds
Limitations/Interferences
• Heparin intake
• Glass tube causes the release of platelet factor IV which is a potent inhibitor of heparin
• Treatments with thrombin inhibitors
• Liver diseases
Frequently Asked Questions (FAQs)
Not applicable
Related Words/Test
TT, Thrombin Clotting Time, Thrombin Time,Prothrombin Time and International Normalized Ratio (PT/INR),Fibrinogen, D-dimer,Thrombin Time, Lupus Anticoagulant Testing, Activated Clotting Time (ACT), Coagulation Factors, Platelet Count, Heparin Anti-Xa, von Willebrand Factor, Antiphospholipid Antibodies, Bleeding Disorders, Excessive Clotting Disorders, Vitamin K Deficiency, Disseminated Intravascular Coagulation (DIC), Antiphospholipid Syndrome, Deep Vein Thrombosis (DVT).
Test
Acylcarnitine
Other Test Request Name
Not applicable
Test Composition
Not applicable
Intended Use
Elevation of one or more acylcarnitines is diagnostic for an organic aciduria or a fatty disorder. A low carnitine level may mask a disease state.
Methodology
Chromatography/Mass Spectrometry
Laboratory Section
Special Test
Special Instructions/Patient Preparations
- Patient should be in a well-fed state
- Collect blood sample in sodium heparin green-top tube. Separate plasma by centrifugation ASAP. Avoid hemolysis. Remove plasma and place in plastic tube.Freeze immediately after separation.Date of birth is required.
Collection/Sample Container
2pcs 4mL Sodium heparin (green-top) tube
Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
4mL Sodium Heparin Plasma
Alternative Specimen and Volume Requirement
Not applicable
Specimen Stability
• Room Temperature (15-25°C)
1 hour
• Refrigerated Temperature (2-8°C)
1 hour
• Freezer Temperature (-20°C)
30 days
Transport Temperature
Transport specimen with Dry Ice
Rejection Criteria
Gross hemolysis
Running Day
Batch Running
Cut Off Time
Monday, 12:00 PM
TAT/Releasing of Results
14 working days (excluding saturday, sunday and holidays)
Reference Interval/Result Interpretation
Not applicable
Limitations/Interferences
Not applicable
Frequently Asked Questions (FAQs)
Not applicable
Related Words/Test
Not Applicable
Test
Anti HBc Total (CMIA)
Other Test Request Name
- Hepatitis B core Total Antibody
- Anti HBc IgG - Anti HBc Total (not specific for IgG) is the replacement test for Anti HBc IgG.
Test Composition
Not applicable
Intended Use
Anti-HBc determinations can be used as an indicator of current or past HBV infection.
Methodology
Chemiluminescent Microparticle Immunoassay (CMIA)
Laboratory Section
Immunology
Special Instructions/Patient Preparations
No patient preparation necessary.
Collection/Sample Container
Red or Gold tube
Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
1-3 mL Serum
Alternative Specimen and Volume Requirement
1-3 mL Plasma (Li-Heparin (PST), Na-Heparin, K-EDTA, Na-citrate, ACD, CPDA-1, CPD, and Potassium oxalate)
Specimen Stability
• Room Temperature (15-25°C)
Not specified
• Refrigerated Temperature (2-8°C)
14 Days
• Freezer Temperature (-20°C)
6 Freeze-Thaw cycles
Transport Temperature
Transport specimen at 2°C – 8 °C (with cold packs)
Rejection Criteria
• Hemolyzed specimen
• Markedly lipemic specimen
• Specimen stored/transported outside the temperature range
• Quantity not sufficient
• Improperly labeled specimens
Running Day
Tuesday, Wednesday, Thursday, Friday, Saturday
Cut Off Time
Monday to Saturday:6:00 PM
TAT/Releasing of Results
ROUTINE (on running days)
• 6 hours after receipt of specimen/ arrival of messenger
STAT (on running days only)
• 2½ hours from extraction/messenger arrival
Reference Interval/Result Interpretation
• NON-REACTIVE: C.O.V. 0.000~0.900
• GRAYZONE: C.O.V. 0.901~1.100
• REACTIVE: C.O.V. GREATER THAN (>) 1.100
Limitations/Interferences
• Specimens from patients who have received preparations of mouse monoclonal antibodies for diagnosis of therapy may contain human anti-mouse antibodies (HAMA). Specimens containing HAMA may produce anomalous values when tested with this assay.
• Hemophilic antibodies in human serum can react with reagent immunoglobulins, interfering with in vitro immunoassays. Patients routinely exposed to animals or to animal serum products can be prone to this interference and anomalous results may be observed. Additional information may be required for diagnosis.
Frequently Asked Questions (FAQs)
Not applicable
Related Words/Test
Hepatitis B, HBV, HBV Tests; Hep B; anti-HBs; Hepatitis B Surface Antibody; HBsAg; Hepatitis B Surface Antigen; HBeAg; Hepatitis B e Antigen; anti-HBc; Hepatitis B Core Antibody; anti-HBc, IgM; anti-HBe; Hepatitis B e Antibody; HBV DNA, Hepatitis B Virus
Test
Allergy Panel(Food)
Other Test Request Name
- IgE against multiple individual allergens
- Food allergens- Serum
- CLA30 - food allergens
- EUROIMMUN EUROLINE Dot
- Specific IgEs - CLA30 - food allergens
Test Composition
Food Allergens
- F13 Peanut
- F20 Almond
- F12 Pea
- F89 Mustard
- F25 Tomato
- F48 Onion
- F49 Apple
- F92 Banana
- F17 Hazel Nut
- F202 Cashew Nut
- F14 Soya Bean
- F96 Avocado
- F47 Garlic
- F85 Celery
- F84 Kiwi
- F95 Peach
- F26 Pork
- F83 Chicken
- F27 Beef
- F3 Codfish
- F24 Shrimp
- F23 Crab
- F40 Tuna
- F4 Wheat Flour
- F9 Rice
- F45 Yeast
- F8 Corn Flour
- F10 Sesame Seed
- F2 Cow's Milk
- F300 Goat's Milk
- F75 Egg Yolk
- F78 nBos D8 - Casein
- F1 Egg White
- CCD Carbohydrate Markers
Intended Use
Quantitative determination of specific IgE against 34 Food Allergens
An Immunoglobulins which mediate immediate-type allergic reactions.
Methodology
Enzymes Immunoassay
Laboratory Section
Special Test
Special Instructions/Patient Preparations
• It is recommended to test after 4-6 weeks of allergic/anaphylactic reaction.
NOTE:
•Inform patient that it is recommended to test after 4-6 weeks of allergic/anaphylactic reaction.
• Regardless if patient requested for only one or less than 20 Food Allergens, still use the respective test codes for the complete panel.
• Patient will receive results for ALL allergens in the list depending upon the type of allergy panel requested.
Collection/Sample Container
Red or Gold tube
Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
3 mL Serum
Alternative Specimen and Volume Requirement
Not Applicable
Specimen Stability
• Room Temperature (15-25°C)
Not Specified
• Refrigerated Temperature (2-8°C)
7 Days
• Freezer Temperature (-20°C)
28 Days
Transport Temperature
Transport specimen at 2 – 8 °C (with cold packs)
Rejection Criteria
• Improperly labeled specimen
• Specimen stored outside the temperature range (2°C to 8°C.)
• Hemolyzed specimen
• Quantity not sufficient
Running Day
Batch Running
Cut Off Time
Friday, 4:00 PM
TAT/Releasing of Results
2 weeks after cut-off (excluding saturdays, sundays & holidays)
Reference Interval/Result Interpretation
Not Applicable
Limitations/Interferences
None specified
Frequently Asked Questions (FAQs)
- Q: Who should get an allergy panel test?
A: All patients who should be tested for specific IgE.
2. Q: When is the best time to test my allergens?
A: It is recommended to test after 4-6 weeks of allergic/anaphylactic reaction.
3. Q: Will antihistamines affect my allergy panel results? Are there other medication restrictions for this test?
A:Antihistamines will never affect any in vitro allergy test results. We have tested a couple of drugs like bilirubin and omalizumab. Omalizumab might have an effect on the results
4. Q: If my test results are negative, does that mean I am not allergic?
A: No. A negative test does not rule out allergy. A negative SPT or serum IgE test does not entirely exclude a diagnosis of a (food) allergy. A positive sIgE test results indicate sensitization, but are not equivalent to clinical allergy. Increasingly higher levels of sIgE generally correlate with an increased risk of clinical allergy. SIgE test results typically do not reflect the severity of allergies (Sicherer and Wood, 2012).
5. Q: Are there any patient preparations/ restrictions before testing?
A: No preparations needed before the test. Fasting or interruption of the treatment with antihistamines is not required.
6. Q: Percent of reliability (specificity and sensitivity)?
A:The measurement of sensitivity is thru limit of detection (LoD).The LoD was determined by using a positive human serum on a panel of 18 single allergens in different concentrations and a negative human reference serum as the limit of blank. All LoDs for the tested single allergens were determined
7. Q: Advantage/disadvantage of Rida QLine against other Allergy Test in the market (in vivo/ in vitro)?
A:Advantages: cost effective; comprehensive panel; quantitative for each allergen composition; batch testing not required; universal reagents (not lot specific); user-friendly; less maintenance; not invasive unlike SPT; ideal for patients unable to withdraw from antihistamines, uncooperative patients, high risk patients.
Disadvantages: manual method; single allergen testing not possible; turn around time longer than SPT
Test
ALP (COLORIMETRIC)
Other Test Request Name
• ALK Phos
• ALKP
• Alkaline Phosphatase
• ALK PO4
Test Composition
Not applicable
Intended Use
• Used to help detect liver disease or bone disorders
• Often used to detect blocked bile ducts because ALP is especially high in the edges of cells that join to form bile ducts
• May be used to help diagnose Paget's disease, a condition that causes malformed bones.
• May also be used to monitor treatment of Paget's disease or other bone conditions, such as vitamin D deficiency
Methodology
Colorimetric
Laboratory Section
Chemistry
Special Instructions/Patient Preparations
8-12 hours fasting.
Note:
• Prolonged fasting is highly discouraged since various metabolic changes associated with starvation may begin to develop. Water intake is acceptable.
• Note in clinical info if patient drank more than 4 glasses of water during fasting period, ask patient for medical history as this is rather unusual.
• Note in clinical info in case patient overfasts and insists on proceeding test.
Collection/Sample Container
Red or Gold tube
Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
1-3 mL Serum
Specimen Stability
• Room Temperature (15-25°C)
7 Days
• Refrigerated Temperature (2-8°C)
7 Days
• Freezer Temperature (-20°C)
2 Months
Transport Temperature
Transport specimen at 2 – 8 °C (with cold packs)
Rejection Criteria
- Improper collection tube used
- Specimen stored/transported outside the temperature range
- Quantity not sufficient
- Failed serum index
- Improperly labeled specimen
- EDTA plasma
Running Day
Daily
Cut Off Time
Monday to Saturday:6:00 PM
Sunday:4:00PM
TAT/Releasing of Results
ROUTINE: 4 hours after receipt of specimen/ arrival of messenger
STAT ON RUNNING DAY ONLY: 2 hours after receipt of specimen/ arrival of messenger
Reference Interval/Result Interpretation
0 Day - 14 Days MALE/FEMALE: 83.00-248.00 U/L
15 Day - < 1 y/o MALE/FEMALE: 122.00-469.00
1 y/o - < 10 y/o MALE/FEMALE: 142.00-335.00
10 y/o - < 13 y/o MALE/FEMALE: 129.00-417.00
13 y/o - < 15 y/o MALE: 116.00-468.00
13 y/o - < 15 y/o FEMALE: 57.00-254.00
15 y/o - < 17 y/o MALE: 82.00-331.00
15 y/o - < 17 y/o FEMALE: 50.00-117.00
17 y/o - < 19 y/o MALE: 55.00-149.00
17 y/o - < 19 y/o FEMALE: 45.00-87.00
19 y/o – 999 y/o MALE: 40.00-129.00
19 y/o – 999 y/o FEMALE: 35.00-104.00
Limitations/Interferences
- In very rare cases, gammopathy, in particular type IgM (Waldenstrom’s macroglobulinemia), may cause unreliable results.
- Interferences from medications or endogenous substances may affect results
Frequently Asked Questions (FAQs)
Not applicable
Related Words/Test
AST, ALT, GGT, Bilirubin, Liver Panel, Bone Markers, Alkaline Phosphatase Isoenzymes, Bone Specific ALP
Test
Alpha 1 Antitrypsin
Other Test Request Name
- Alpha-1 Antitrypsin
- A1 Antitrypsin
- Pi - Protease Inhibitor
- Protease Inhibitor
- A1AT
- AAT
Test Composition
Not Applicable
Intended Use
Alpha-1-antitrypsin (A1AT) is a glycoprotein synthesized in the liver which is found in most biological fluids and in the feces. It inactivates a wide range of proteolytic enzymes including elastase, chymotrypsin, trypsin and cathepsin. It is also involved in inhibiting Factor XIIa and kallikrein Biochemical polymorphisms of A1AT constitute the Pi (Protease inhibitor) system.A1AT levels rise during pregnancy and can fall as a result of heavy consumption at a focus of inflammation. Congenital deficiencies in this enzyme are associated with variably decreased levels in the serum.Alpha-1 antitrypsin (AAT) is a protein in the blood that protects the lungs from damage caused by activated enxymes. Laboratory tests measure the level of AAT in blood or identify abnormal forms of AAT that a person has inherited to help diagnose alpha-1 antitrypsin deficiency.
Methodology
Nephelometry
Laboratory Section
Special Test
Special Instructions/Patient Preparations
- No patient preparation necessary.
- Please specify the patient gender, age and the possible treatment (ALFALASTIN)
Collection/Sample Container
Red or Gold tube
Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
3 mL Serum
Alternative Specimen and Volume Requirement
Not Applicable
Specimen Stability
• Room Temperature (15-25°C)
24 Hours
• Refrigerated Temperature (2-8°C)
7 Days
• Freezer Temperature (-20°C)
28 Days
Transport Temperature
Transport specimen at 2 – 8 °C (with cold packs)
Rejection Criteria
- Hemolyzed specimen
- Quantity Not sufficient
- Insufficient volume
- Markedly lipemic sample
- Improperly labeled specimen
Running Day
Batch Running
Cut Off Time
Friday, 4:00 PM
TAT/Releasing of Results
2 weeks after cut-off (excluding Saturdays, Sundays and Holidays)
Reference Interval/Result Interpretation
Available upon request
None specified.
Frequently Asked Questions (FAQs)
Q: Why get tested?
A:To help diagnose the cause of early onset emphysema and/or liver dysfunction; to establish the risk of emphysema and/or liver disease due to alpha-1 antitrypsin deficiency and determine the likelihood that children might inherit the risk
Q: When to get tested?
A:When your infant or young child show signs of liver disease; when you develop emphysema before age 40; when you have chronic obstructive pulmonary disease (COPD) or unexplained liver disease at any age; when you have a close relative with alpha-1 antitrypsin deficiency
Q: When is it ordered?
A:Alpha-1 antitrypsin testing may be ordered when:
- An infant has jaundice that lasts for more than a week or two, an enlarged spleen, fluid accumulation in the abdomen (ascites), persistent itching (pruritis), and other signs of liver injury
- Someone of any age has COPD, raised, tender skin spots that form ulcers (necrotizing panniculitis), a vascular disease called granulomatosis with polyangiitis, or unexplained airway widening (bronchiectasis)
- A person younger than 40 years of age develops wheezing, a chrmonic cough or bronchitis, is short of breath after exertion, and/or shows other signs of emphysema; this is especially true when the person is not a smoker, has not been exposed to known lung irritants, and when the lung damage appears to be located low in the lungs.
- Someone has a close relative with alpha-1 antitrypsin deficiency
- An individual has an affected family member and wants to know the likelihood of having an affected child
Q:What can I do to take care of myself if I have alpha-1antitrypsin (AAT) deficiency)?
A:Don’t smoke. Taking care of your lungs can increase your lifespan and delay the onset of emphysema. Avoid lung irritants such as dust and fumes, get regular vaccinations to protect against pneumococcal pneumonia andHaemophilus influenzaetype b infections, for example, and an annual influenza shot, get prompt medical attention for lung infections, and get regular exercise to help maintain lung function. Work with your healthcare practitioner to determine what steps will help you take care of yourself.
Related Words/Test
Emphysema in adults, rare form of liver disease (cirrhosis), Protein Electrophoresis, Immunofixation Electrophoresis, Total Protein, Albumin-Globulin (A/G) Ratio, Blood Gases, Liver Panel.
Test
Alpha 2 Macroglobulin
Other Test Request Name
• A2M
Test Composition
Not applicable
Intended Use
Synthesized by the cells of the hepatic parenchyma, Alpha2-Macroglobulin is involved in inhibition of enzymes, complement, coagulation and fibrinolytic systems. An increase in serum alpha2- macroglobulin is associated with nephrotic syndrome, estrogen stimulation as in pregnancy or with oral contraceptive use. While a decrease indicates presence of pancreatitis, liver disease, and prostate carcinoma.
Methodology
Immunotubidimetry
Laboratory Section
Chemistry
Special Instructions/Patient Preparations
• No patient preparation necessary.
Red or Gold tube
Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
1-3 mL Serum
Alternative Specimen and Volume Requirement
1-3mL Plasma (Li-Heparin)
Specimen Stability
• Room Temperature (15-25°C)
24 Hours
• Refrigerated Temperature (2-8°C)
7 Days
• Freezer Temperature (-20°C)
3 Months
Transport Temperature
Transport specimen at 2 – 8 °C (with cold packs)
Rejection Criteria
• Hemolyzed specimen
• Markedly lipemic specimen
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used
Running Day
Wednesday and Saturday
Cut Off Time
Monday to Saturday: 6:00PM
Sunday: 4:00PM
TAT/Releasing of Results
ROUTINE
• 4 hours after receipt of specimen/ arrival of messenger
STAT
• After 2½ hours from extraction/messenger arrival
Reference Interval/Result Interpretation
Male: 1.50-3.50 g/L (150.00-350.00 mg/dL)
Female: 1.75-4.20 g/L (175.00-420.00 mg/dL)
Limitations/Interferences
Bilirubin (conjugated/unconjugated) >500 umol/L
Hemolysis >120 umol/L
Lipemia >15.1 mmol/L
Frequently Asked Questions (FAQs)
Not applicable
Related Words/Test
LiverFast
Test
Alpha Globin Genotyping
Other Test Request Name
Not applicable
Test Composition
Not applicable
Intended Use
Not Applicable
Methodology
Genotyping
Laboratory Section
Special Test
Special Instructions/Patient Preparations
- For schedule
- Sample must be collected every Tuesday
- Need Updated CBC (within 1 month) and HGB ELECTROPHORESIS result (within 1 month)
Collection/Sample Container
EDTA
Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.
3 pcs 4mL EDTA Wholeblood
Alternative Specimen and Volume Requirement
Not Applicable
Specimen Stability
• Room Temperature (15-25°C)
24 Hours
• Refrigerated Temperature (2-8°C)
24 Hours
• Freezer Temperature (-20°C)
Not Applicable
Transport Temperature
Transport specimen at 2 – 8 °C (with cold packs)
Rejection Criteria
- Improperly labeled specimens
- Clotted specimens
- Over-filled or Under-filled tube
- Quantity not sufficient
- Specimen stored and transported outside the required temperature
Running Day
Wednesday
Cut Off Time
Tuesday 7am
TAT/Releasing of Results
3 Working days (excluding saturdays, sundays and holidays)
Reference Interval/Result Interpretation
Not Applicable
Limitations/Interferences
Not Applicable
Frequently Asked Questions (FAQs)
Not Applicable
Related Words/Test
Not Applicable