Laboratory Test Preparation

Not Applicable

Immunohistochem

Special Test

No patient preparation

Doctors Order is needed

Slide holders or mailers for microscope slides; FFPE tissue blocks can either be wrapped in paper or placed in a box.

• Formalin-fixed paraffin-embedded (FFPE) tissue block
• 1 H&E stained slide representative of the submitted tissue block.
• Histopathology report for the submitted tissue block

Not Applicable

Indefinite

Not applicable

Not applicable

• Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.
• FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.
• Transport specimen at 15°C ~25 °C (room temperature)

• Incomplete information in the Request Form
• Improper or insufficient labeling of tissue blocks or slides
• Inadequate tissue available on the tissue block

Monday - Friday

A day before running day

10 days (excluding Saturdays, Sundays and Holidays)

Not applicable

Not applicable

Not applicable

Not applicable

Not applicable

Not applicable

H-Caldesmon, a calcium-binding protein mediating smooth muscle contraction, is predominantly expressed in smooth muscle and a subset of myoepithelial cells.

Immuno Histochemistry

Special Test

No patient preparation necessary.

Slide holders or mailers for microscope slides; FFPE tissue blocks can either be wrapped in paper or placed in a box.

• Formalin-fixed paraffin-embedded (FFPE) tissue block
• 1 H&E stained slide representative of the submitted tissue block.
• Histopathology report for the submitted tissue block

Not Applicable

Indefinite

Not applicable

Not applicable

• Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.
• FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.
• Transport specimen at 15°C ~25 °C (room temperature)

• Incomplete information in the Request Form
• Improper or insufficient labeling of tissue blocks or slides
• Inadequate tissue available on the tissue block

Monday to Friday

Day before running day

10 days (excluding Saturdays, Sundays and Holidays)

Not Applicable

None specified.

Not applicable

Not applicable

Not applicable

• Calretinin is a diagnostic marker for some human disease, including Hirschsprung disease and some cancers.

Immuno Histochemistry

Special Test

No patient preparation necessary.

Slide holders or mailers for microscope slides; FFPE tissue blocks can either be wrapped in paper or placed in a box.

• Formalin-fixed paraffin-embedded (FFPE) tissue block
• 1 H&E stained slide representative of the submitted tissue block.
• Histopathology report for the submitted tissue block

Not Applicable

Indefinite

Not applicable

Not applicable

• Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.
• FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.
• Transport specimen at 15°C ~25 °C (room temperature)

• Incomplete information in the Request Form
• Improper or insufficient labeling of tissue blocks or slides
• Inadequate tissue available on the tissue block

Monday to Friday

Day before running day

10 days (excluding Saturdays, Sundays and Holidays)

Not Applicable

None specified.

Not applicable

Not applicable

Not applicable

• The following CD markers are expressed on specific hematologic cells. Individual and a series of IHC markers can be run on unknown cells and presence or absence of the antigen can help to characterize the cell of interest.

Immuno Histochemistry

Special Test

No patient preparation necessary.

Slide holders or mailers for microscope slides; FFPE tissue blocks can either be wrapped in paper or placed in a box.

• Formalin-fixed paraffin-embedded (FFPE) tissue block
• 1 H&E stained slide representative of the submitted tissue block.
• Histopathology report for the submitted tissue block

Indefinite

Not applicable

Not applicable

• Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.
• FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.
• Transport specimen at 15°C ~25 °C (room temperature)

• Incomplete information in the Request Form
• Improper or insufficient labeling of tissue blocks or slides
• Inadequate tissue available on the tissue block

Monday to Friday

Day before running day

10 days (excluding Saturdays, Sundays and Holidays)

Not Applicable

None specified.

Not applicable

Anti-c-KIT

Not applicable

- Used as an aid in the diagnosis of GIST within the context of the patient's clinical history, tumor morphology, and other diagnostic tests evaluated by a qualified pathologist.

- Used as an aid in the selection of GIST patients who may qualify for imatinib mesylate (Gleevec®/Glivec®) therapy.

Immunohistochemistry (IHC)

Histopathology

All specimens must be LABELED properly and legibly. The label must match the information on the test request form and on the provided histopathology report

- Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.

- FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.

- Formalin-fixed paraffin-embedded (FFPE) tissue block

- H&E stained slide, representative of the submitted tissue block.

- Histopathology report for the submitted tissue block and slide

- Completely filled out HPD Histopathology Request Form (HPD_F_258)

Note:Submit to HPD Main Laboratory all tissue blocks and slides received.

Not Applicable

- FFPE tissue blocks and H&E slides are stable indefinitely when stored properly at room temperature (15^oC~30^oC).

- Hi-Precision Diagnostics will store FFPE tissue blocks and H&E slides for 10 years, after which samples will be disposed properly according to biosafety disposal guidelines.

Indefinite

Not applicable

Not applicable

Transport specimen at 15^oC -30^oC

- Incorrect/Incomplete information in the HPD Histopathology Request Form

- Improper or insufficient labeling of tissue blocks or slides

- Inadequate tissue available on the tissue block

- Tissue blocks and slides do not correspond to its histopathology report

- Specimen stored and transported outside the required temperature

Monday to Saturday

12:00 PM

- With Reading - 3 to 5 days after receipt of specimen/ messengers arrival or 10 working days (if with special test)

- For Processing Only - 1 day after receipt of specimen/ messengers arrival

Note: Additional 2 days may be necessary if specimen needs further evaluation and confirmation by another Pathologist

- The clinical interpretation of any staining, or absence of staining, must be complemented by histological studies and evaluation of proper controls.

- Evaluation must be made by a qualified pathologist within the context of the patient’s clinical history and other diagnostic tests.

- Ventana recommends fixation in 10% NBF for 6-48 hours.

- Fixation for less than 6 hours is not recommended.

Q. What is Immunohistochemistry?

A.Immunohistochemistry or IHC refers to the process of detecting antigens (e.g., proteins) in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues.

Q. Why do we need to undergo Immunohistochemistry test?

A. - Confirm a pathologic diagnosis

      - Differentiate between benign or malignant tumor

      - Detect among different types of tumor

      - Monitor prognosis response to treatment

- ImmunoHistoChemistry (IHC)

- Anti-c-KIT (9.7)

- GIST

Not applicable

The following CD markers are expressed on specific hematologic cells. Individual and a series of IHC markers can be run on unknown cells and presence or absence of the antigen can help to characterize the cell of interest.

Immuno Histochemistry

Special Test

No patient preparation necessary.

Slide holders or mailers for microscope slides; FFPE tissue blocks can either be wrapped in paper or placed in a box.

• Formalin-fixed paraffin-embedded (FFPE) tissue block
• 1 H&E stained slide representative of the submitted tissue block.
• Histopathology report for the submitted tissue block

Not Applicable

Indefinite

Not applicable

Not applicable

• Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.
• FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.
• Transport specimen at 15°C ~25 °C (room temperature)

• Incomplete information in the Request Form
• Improper or insufficient labeling of tissue blocks or slides
• Inadequate tissue available on the tissue block

Monday to Friday

Day before running day

10 days (excluding Saturdays, Sundays and Holidays)

Not Applicable

None specified.

Not applicable

- anti-CD15 (MMA)

- lacto-N-fucopentose III

Not applicable

- In haematopathology CD15 is important for the diagnosis of classical HD and characterization of acute leukemia. In the differentiation of mesothelioma vs. adenocarcinoma, CD15 can be used in the primary panel.

- Hepatocellular, gastric and colonic carcinoma and thyroid medullary carcinoma appear to have worse prognosis if positive for CD15.

- CD15 may be used for histopathological grading of gliomas and differentiating between malignant gliomas and non-neoplastic glial cells (the latter usually strongly stained).

- CD15 is also a pertinent panel marker for thymoma, myxoinflammatory fibroblastic sarcoma, histiocytic lymphoma-sarcoma, peripheral T-cell lymphomas, anaplastic large cell lymphoma, and renal cell carcinoma.

Immunohistochemistry (IHC)

Histopathology

All specimens must be LABELED properly and legibly. The label must match the information on the test request form and on the provided histopathology report

- Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.

- FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.

- Formalin-fixed paraffin-embedded (FFPE) tissue block

- H&E stained slide, representative of the submitted tissue block.

- Histopathology report for the submitted tissue block and slide

- Completely filled out HPD Histopathology Request Form (HPD_F_258)

Note:Submit to HPD Main Laboratory all tissue blocks and slides received.

Not Applicable

- FFPE tissue blocks and H&E slides are stable indefinitely when stored properly at room temperature (15^oC~30^oC).

- Hi-Precision Diagnostics will store FFPE tissue blocks and H&E slides for 10 years, after which samples will be disposed properly according to biosafety disposal guidelines.

Indefinite

Not applicable

Not applicable

Transport specimen at 15^oC -30^oC

- Incorrect/Incomplete information in the HPD Histopathology Request Form

- Improper or insufficient labeling of tissue blocks or slides

- Inadequate tissue available on the tissue block

- Tissue blocks and slides do not correspond to its histopathology report

- Specimen stored and transported outside the required temperature

Monday to Saturday

12:00 PM

- With Reading - 3 to 5 days after receipt of specimen/ messengers arrival or 10 working days (if with special test)

- For Processing Only - 1 day after receipt of specimen/ messengers arrival

Note: Additional 2 days may be necessary if specimen needs further evaluation and confirmation by another Pathologist

- The clinical interpretation of any staining, or absence of staining, must be complemented by histological studies and evaluation of proper controls.

- Evaluation must be made by a qualified pathologist within the context of the patient’s clinical history and other diagnostic tests.

- Ventana recommends fixation in 10% NBF for 6-48 hours.

- Fixation for less than 6 hours is not recommended.

Q. What is Immunohistochemistry?

A.Immunohistochemistry or IHC refers to the process of detecting antigens (e.g., proteins) in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues.

Q. Why do we need to undergo Immunohistochemistry test?

A. - Confirm a pathologic diagnosis

      - Differentiate between benign or malignant tumor

      - Detect among different types of tumor

      - Monitor prognosis response to treatment

- ImmunoHistoChemistry (IHC)

- anti-CD15 (MMA)

- lacto-N-fucopentose III

- Lymphoma

CD20

Not applicable

- As part of the basic IHC panel in lymphoma diagnosis

- One of the most important markers for the identification of B-cell neoplasms.

- CD20 is expressed in the large majority of cases of B-cell leukaemia/lymphoma.

Immunohistochemistry (IHC)

Histopathology

All specimens must be LABELED properly and legibly. The label must match the information on the test request form and on the provided histopathology report.

- Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.

- FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.

- Formalin-fixed paraffin-embedded (FFPE) tissue block

- H&E stained slide, representative of the submitted tissue block.

- Histopathology report for the submitted tissue block and slide

- Completely filled out HPD Histopathology Request Form (HPD_F_258)

Note:Submit to HPD Main Laboratory all tissue blocks and slides received.

Not applicable

- FFPE tissue blocks and H&E slides are stable indefinitely when stored properly at room temperature (15^oC~30^oC).

- Hi-Precision Diagnostics will store FFPE tissue blocks and H&E slides for 10 years, after which samples will be disposed properly according to biosafety disposal guidelines.

Indefinite

Not applicable

Not applicable

Transport specimen at 15°C to 30°C

- Incorrect/Incomplete information in the HPD Histopathology Request Form

- Improper or insufficient labeling of tissue blocks or slides

- Inadequate tissue available on the tissue block

- Tissue blocks and slides do not correspond to its histopathology report

- Specimen stored and transported outside the required temperature

Monday to Saturday

12:00 PM

- With Reading - 3 to 5 days after receipt of specimen/ messengers arrival or 10 working days (if with special test)

- For Processing Only - 1 day after receipt of specimen/ messengers arrival

Note: Additional 2 days may be necessary if specimen needs further evaluation and confirmation by another Pathologist

- The clinical interpretation of any staining, or absence of staining, must be complemented by histological studies and evaluation of proper controls.

- Evaluation must be made by a qualified pathologist within the context of the patient’s clinical history and other diagnostic tests.

- Ventana recommends fixation in 10% NBF for 6-48 hours.

- Fixation for less than 6 hours is not recommended.

Q. What is Immunohistochemistry?

A.Immunohistochemistry or IHC refers to the process of detecting antigens (e.g., proteins) in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues.

Q. Why do we need to undergo Immunohistochemistry test?

A. - Confirm a pathologic diagnosis

      - Differentiate between benign or malignant tumor

      - Detect among different types of tumor

      - Monitor prognosis response to treatment

- Immunohistochemistry (IHC)

- Lymphoma panel

- CD20

• CD23 Immunohistochemistry
• Fc epsilon RII
• FcεRII

Not Applicable

Identification of follicular dendritic cells

Clasifications of low-grade B-cell lymphomas

Immunohistochemistry (IHC)

Special test

• All specimens must be LABELED properly and legibly.
• The label must match the information on the test request form and on the provided histopathology report.

.

• Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.
• FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.

Formalin-fixed paraffin-embedded (FFPE) tissue block:

• H&E stained slide, representative of the submitted tissue block.
• Histopathology report for the submitted tissue block and slide
• Completely filled out HPD Histopathology Request Form (HPD_F_258)

Note:Submit to HPD Main Laboratory all tissue blocks and slides received.

Not Applicable

Indefinite

Not Applicable

Not Applicable

Transport specimen at 15^oC -30^oC

• Incorrect/Incomplete information in the HPD Histopathology Request Form
• Improper or insufficient labeling of tissue blocks or slides
• Inadequate tissue available on the tissue block
• Tissue blocks and slides do not correspond to its histopathology report
• Specimen stored and transported outside the required temperature

Monday, Wednesday and Friday

a day before running day. 7:00 AM

10 working days after running day (excluding saturdays, sundays and holidays)

• The clinical interpretation of any staining, or absence of staining, must be complemented by histological studies and evaluation of proper controls.
• Evaluation must be made by a qualified pathologist within the context of the patient’s clinical history and other diagnostic tests.

Not Applicable

Not Applicable

Not Applicable

CD3

Not applicable

- As part of the basic IHC panel in lymphoma diagnosis

- Pan T-cell marker, late thymocytes and mature T-cells.

- Expressed on many neoplasms of mature T cells.

- There is surface membrane expression in a few cases of precursor T-cell leukaemia/lymphoma and cytoplasmic expression in most cases

Immunohistochemistry (IHC)

Histopathology

All specimens must be LABELED properly and legibly. The label must match the information on the test request form and on the provided histopathology report.

- Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.

- FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.

- Formalin-fixed paraffin-embedded (FFPE) tissue block

- H&E stained slide, representative of the submitted tissue block.

- Histopathology report for the submitted tissue block and slide

- Completely filled out HPD Histopathology Request Form (HPD_F_258)

Note:Submit to HPD Main Laboratory all tissue blocks and slides received.

Not applicable

- FFPE tissue blocks and H&E slides are stable indefinitely when stored properly at room temperature (15^oC~30^oC).

- Hi-Precision Diagnostics will store FFPE tissue blocks and H&E slides for 10 years, after which samples will be disposed properly according to biosafety disposal guidelines.

Indefinite

Not applicable

Not applicable

Transport specimen at 15°C to 30°C

- Incorrect/Incomplete information in the HPD Histopathology Request Form

- Improper or insufficient labeling of tissue blocks or slides

- Inadequate tissue available on the tissue block

- Tissue blocks and slides do not correspond to its histopathology report

- Specimen stored and transported outside the required temperature

Monday to Saturday

12:00 PM

- With Reading - 3 to 5 days after receipt of specimen/ messengers arrival or 10 working days (if with special test)

- For Processing Only - 1 day after receipt of specimen/ messengers arrival

Note: Additional 2 days may be necessary if specimen needs further evaluation and confirmation by another Pathologist

- The clinical interpretation of any staining, or absence of staining, must be complemented by histological studies and evaluation of proper controls.

- Evaluation must be made by a qualified pathologist within the context of the patient’s clinical history and other diagnostic tests.

- Ventana recommends fixation in 10% NBF for 6-48 hours.

- Fixation for less than 6 hours is not recommended.

Q. What is Immunohistochemistry?

A.Immunohistochemistry or IHC refers to the process of detecting antigens (e.g., proteins) in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues.

Q. Why do we need to undergo Immunohistochemistry test?

A. - Confirm a pathologic diagnosis

      - Differentiate between benign or malignant tumor

      - Detect among different types of tumor

      - Monitor prognosis response to treatment

- Immunohistochemistry (IHC)

- Lymphoma panel

- CD3

CD30

Not applicable

- As part of the basic IHC panel in lymphoma diagnosis

- Activated T and B lymphoid cell marker; also stains embryonal carcinoma and malignant melanoma cells.

- Expressed in Reed-Sternberg cells and Hodgkin cells in classical Hodgkin lymphoma, anaplastic large cell lymphoma, some diffuse large B-cell lymphomas (in particular mediastinal B-cell lymphoma), plasmablastic lymphoma and some cases of primary effusion lymphoma

Immunohistochemistry (IHC)

Histopathology

All specimens must be LABELED properly and legibly. The label must match the information on the test request form and on the provided histopathology report.

- Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.

- FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.

- Formalin-fixed paraffin-embedded (FFPE) tissue block

- H&E stained slide, representative of the submitted tissue block.

- Histopathology report for the submitted tissue block and slide

- Completely filled out HPD Histopathology Request Form (HPD_F_258)

Note:Submit to HPD Main Laboratory all tissue blocks and slides received.

Not applicable

- FFPE tissue blocks and H&E slides are stable indefinitely when stored properly at room temperature (15^oC~30^oC).

- Hi-Precision Diagnostics will store FFPE tissue blocks and H&E slides for 10 years, after which samples will be disposed properly according to biosafety disposal guidelines.

Indefinite

Not applicable

Not applicable

Transport specimen at 15°C to 30°C

- Incorrect/Incomplete information in the HPD Histopathology Request Form

- Improper or insufficient labeling of tissue blocks or slides

- Inadequate tissue available on the tissue block

- Tissue blocks and slides do not correspond to its histopathology report

- Specimen stored and transported outside the required temperature

Monday to Saturday

12:00 PM

- With Reading - 3 to 5 days after receipt of specimen/ messengers arrival or 10 working days (if with special test)

- For Processing Only - 1 day after receipt of specimen/ messengers arrival

Note: Additional 2 days may be necessary if specimen needs further evaluation and confirmation by another Pathologist

- The clinical interpretation of any staining, or absence of staining, must be complemented by histological studies and evaluation of proper controls.

- Evaluation must be made by a qualified pathologist within the context of the patient’s clinical history and other diagnostic tests.

- Ventana recommends fixation in 10% NBF for 6-48 hours.

- Fixation for less than 6 hours is not recommended.

Q. What is Immunohistochemistry?

A.Immunohistochemistry or IHC refers to the process of detecting antigens (e.g., proteins) in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues.

Q. Why do we need to undergo Immunohistochemistry test?

A. - Confirm a pathologic diagnosis

      - Differentiate between benign or malignant tumor

      - Detect among different types of tumor

      - Monitor prognosis response to treatment

- Immunohistochemistry (IHC)

- Lymphoma panel

- CD30

Not Applicable

Not Applicable

Immuno Histochemistry

Special Test

No patient preparation necessary.

Slide holders or mailers for microscope slides; FFPE tissue blocks can either be wrapped in paper or placed in a box.

• Formalin-fixed paraffin-embedded (FFPE) tissue block
• 1 H&E stained slide representative of the submitted tissue block.
• Histopathology report for the submitted tissue block

Not Applicable

Indefinite

Not Applicable

Not Applicable

• Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.
• FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.
• Transport specimen at 15°C ~25 °C (room temperature)

• Incomplete information in the Request Form
• Improper or insufficient labeling of tissue blocks or slides
• Inadequate tissue available on the tissue block

Tuesday and Thursday

A day before running day

10 working days except saturday, sunday and Holidays

Not Applicable

Not Applicable

Not Applicable

Not Applicable

CD34

Not applicable

- Facilitates identification of blasts in cases of acute leukemia.

- Used as a marker for endothelial cells in the differential diagnosis of poorly differentiated malignant tumors.

- Used as part of a panel in determining the cell lineage of mesenchymal tumors

Immunohistochemistry (IHC)

Histopathology

All specimens must be LABELED properly and legibly. The label must match the information on the test request form and on the provided histopathology report.

- Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.

- FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.

- Formalin-fixed paraffin-embedded (FFPE) tissue block

- H&E stained slide, representative of the submitted tissue block.

- Histopathology report for the submitted tissue block and slide

- Completely filled out HPD Histopathology Request Form (HPD_F_258)

Note:Submit to HPD Main Laboratory all tissue blocks and slides received.

Not applicable

- FFPE tissue blocks and H&E slides are stable indefinitely when stored properly at room temperature (15^oC~30^oC).

- Hi-Precision Diagnostics will store FFPE tissue blocks and H&E slides for 10 years, after which samples will be disposed properly according to biosafety disposal guidelines.

Indefinite

Not applicable

Not applicable

Transport specimen at 15°C to 30°C

- Incorrect/Incomplete information in the HPD Histopathology Request Form

- Improper or insufficient labeling of tissue blocks or slides

- Inadequate tissue available on the tissue block

- Tissue blocks and slides do not correspond to its histopathology report

- Specimen stored and transported outside the required temperature

Monday to Saturday

12:00 PM

- With Reading - 3 to 5 days after receipt of specimen/ messengers arrival or 10 working days (if with special test)

- For Processing Only - 1 day after receipt of specimen/ messengers arrival

Note: Additional 2 days may be necessary if specimen needs further evaluation and confirmation by another Pathologist

- The clinical interpretation of any staining, or absence of staining, must be complemented by histological studies and evaluation of proper controls.

- Evaluation must be made by a qualified pathologist within the context of the patient’s clinical history and other diagnostic tests.

- Ventana recommends fixation in 10% NBF for 6-48 hours.

- Fixation for less than 6 hours is not recommended.

Q. What is Immunohistochemistry?

A.Immunohistochemistry or IHC refers to the process of detecting antigens (e.g., proteins) in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues.

Q. Why do we need to undergo Immunohistochemistry test?

A. - Confirm a pathologic diagnosis

      - Differentiate between benign or malignant tumor

      - Detect among different types of tumor

      - Monitor prognosis response to treatment

- Immunohistochemistry (IHC)

- Mesenchymal tumors

- CD34

Not applicable

Not applicable

The following CD markers are expressed on specific hematologic cells. Individual and a series of IHC markers can be run on unknown cells and presence or absence of the antigen can help to characterize the cell of interest.

Immunohistochemistry (IHC)

Special Test

All specimens must be LABELED properly and legibly. The label must match the information on the test request form and on the provided histopathology report.

- Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.

- FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.

- Formalin-fixed paraffin-embedded (FFPE) tissue block

- H&E stained slide, representative of the submitted tissue block.

- Histopathology report for the submitted tissue block and slide

Not applicable

Indefinite

Not applicable

Not applicable

Transport specimen at 15°C to 30°C

- Improper or insufficient labeling of tissue blocks or slides

- Inadequate tissue available on the tissue block

- Tissue blocks and slides do not correspond to its histopathology report

- Specimen stored and transported outside the required temperature

Monday, Wednesday and Friday

Tuesday and Thursday 7am

10 working days excluding saturday, sunday and holidays

Not applicable

Not applicable

Not applicable