Laboratory Test Preparation

LCA (Leukocyte common antigen)

Not applicable

- As part of the basic IHC panel in lymphoma diagnosis

- CD45 is an important marker in the primary tumour screening panel in order to identify haematolymphoid differentiation.

- Identification of poorly differentiated neoplasms as lymphomas

Immunohistochemistry (IHC)

Histopathology

All specimens must be LABELED properly and legibly. The label must match the information on the test request form and on the provided histopathology report.

- Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.

- FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.

- Formalin-fixed paraffin-embedded (FFPE) tissue block

- H&E stained slide, representative of the submitted tissue block.

- Histopathology report for the submitted tissue block and slide

- Completely filled out HPD Histopathology Request Form (HPD_F_258)

Note:Submit to HPD Main Laboratory all tissue blocks and slides received.

Not applicable

- FFPE tissue blocks and H&E slides are stable indefinitely when stored properly at room temperature (15^oC~30^oC).

- Hi-Precision Diagnostics will store FFPE tissue blocks and H&E slides for 10 years, after which samples will be disposed properly according to biosafety disposal guidelines.

Indefinite

Not applicable

Not applicable

Transport specimen at 15^oC -30^oC

- Incorrect/Incomplete information in the HPD Histopathology Request Form

- Improper or insufficient labeling of tissue blocks or slides

- Inadequate tissue available on the tissue block

- Tissue blocks and slides do not correspond to its histopathology report

- Specimen stored and transported outside the required temperature

Monday to Saturday

12:00 PM

- With Reading - 3 to 5 days after receipt of specimen/ messengers arrival or 10 working days (if with special test)

- For Processing Only - 1 day after receipt of specimen/ messengers arrival

Note: Additional 2 days may be necessary if specimen needs further evaluation and confirmation by another Pathologist

- The clinical interpretation of any staining, or absence of staining, must be complemented by histological studies and evaluation of proper controls.

- Evaluation must be made by a qualified pathologist within the context of the patient’s clinical history and other diagnostic tests.

- Ventana recommends fixation in 10% NBF for 6-48 hours.

- Fixation for less than 6 hours is not recommended.

Q. What is Immunohistochemistry?

A.Immunohistochemistry or IHC refers to the process of detecting antigens (e.g., proteins) in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues.

Q. Why do we need to undergo Immunohistochemistry test?

A. - Confirm a pathologic diagnosis

      - Differentiate between benign or malignant tumor

      - Detect among different types of tumor

      - Monitor prognosis response to treatment

- Immunohistochemistry (IHC)

- Lymphoma panel

- Leukocyte common antigen

- LCA

Not applicable

Not applicable

Immuno Histochemistry

Special Test

• All specimens must be LABELED properly and legibly.
• The label must match the information on the test request form and on the provided histopathology report.

• Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.
• FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.

Formalin-fixed paraffin-embedded (FFPE) tissue block:

• H&E stained slide, representative of the submitted tissue block.
• Histopathology report for the submitted tissue block and slide
• Completely filled out HPD Histopathology Request Form (HPD_F_258)

Note:Submit to HPD Main Laboratory all tissue blocks and slides received.

Not applicable

Not Applicable

Indefinite

Not Applicable

Not Applicable

Transport specimen at 15^oC -30^oC

• Incorrect/Incomplete information in the HPD Histopathology Request Form
• Improper or insufficient labeling of tissue blocks or slides
• Inadequate tissue available on the tissue block
• Tissue blocks and slides do not correspond to its histopathology report
• Specimen stored and transported outside the required temperature

Tuesday and Thursday

Monday & Wednesday 7am

10 working days (excluding Saturday, Sunday and Holidays)

IHC-CD 68

Not Applicable

Useful as a marker for the various cells of the macrophage lineage, including monocytes, histiocytes, giant cells, Kupffer cells, and osteoclasts.

Used to distinguish diseases of otherwise similar appearance, such as the monocyte/macrophage and lymphoid forms of leukaemia (the latter being CD68 negative).

Its presence in macrophages also makes it useful in diagnosing conditions related to proliferation or abnormality of these cells, such as malignant histiocytosis, histiocytic lymphoma, and Gaucher's disease.

Immuno Histochemistry

Special Test

No patient preparation necessary.

Slide holders or mailers for microscope slides; FFPE tissue blocks can either be wrapped in paper or placed in a box

• Formalin-fixed paraffin-embedded (FFPE) tissue block
• 1 H&E stained slide representative of the submitted tissue block.
• Histopathology report for the submitted tissue block
• Completely filled HPD Histopathology Request Form (HPD_F_258)

Not Applicable

Indefinite

Not applicable

Not applicable

• mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.
• FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.
• Transport specimen at 15°C ~25 °C (room temperature)

• Incomplete information in the Request Form
• Improper or insufficient labeling of tissue blocks or slides
• Inadequate tissue available on the tissue block

Mondays, Wednesdays and Fridays

Day before running day

10 Days (excluding Saturdays, Sundays and Holidays)

Not Applicable

None specified.

Not Applicable

Antibody directed against lysosomes

Not applicable

Not applicable

The following CD markers are expressed on specific hemotologic cells. Individual and a series of IHC markers can be run on unknown cells and presence or absence of the antigen can help to characterize the cell of interest.

Immuno Histochemistry

Special Test

No patient preparation necessary.

Slide holders or mailers for microscope slides; FFPE tissue blocks can either be wrapped in paper or placed in a box.

• Formalin-fixed paraffin-embedded (FFPE) tissue block
• 1 H&E stained slide representative of the submitted tissue block.
• Histopathology report for the submitted tissue block

Not Applicable

Indefinite

Not applicable

Not applicable

• Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.
• FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.
• Transport specimen at 15°C ~25 °C (room temperature)

• Incomplete information in the Request Form
• Improper or insufficient labeling of tissue blocks or slides
• Inadequate tissue available on the tissue block

Monday to Friday

Day before running day

10 days (excluding Saturdays, Sundays and Holidays)

Not Applicable

None specified.

Not applicable

• MIC2

• Single Chain Type 1 Glycoprotein

Not applicable

• Useful in diagnosis of Ewing’s sarcoma, sex cord-stromal tumor, endocrine tumor of pancreas. Additionally, it is found in a subset of other tumors including lymphoblastic lymphoma, breast carcinoma and other malignancies.

Immuno Histochemistry

Special Test

No patient preparation necessary.

Slide holders or mailers for microscope slides; FFPE tissue blocks can either be wrapped in paper or placed in a box.

• Formalin-fixed paraffin-embedded (FFPE) tissue block
• 1 H&E stained slide representative of the submitted tissue block.
• Histopathology report for the submitted tissue block

Not Applicable

Indefinite

Not applicable

Not applicable

• Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.
• FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.
• Transport specimen at 15°C ~25 °C (room temperature)

• Incomplete information in the Request Form
• Improper or insufficient labeling of tissue blocks or slides
• Inadequate tissue available on the tissue block

Monday to Friday

Day before running day

10 days (excluding Saturdays, Sundays and Holidays)

Not Applicable

None specified.

Not applicable

Not applicable

• CDX2is a homeobox domain–containing transcription factor that is important in the development and differentiation of the intestine and is expressed in colorectal carcinoma
• CDX2is also seen in mucinous tumors with gastrointestinal differentiation that originate in the lung or ovary.

Immuno Histochemistry

Special test

No patient preparation

Slide holders or mailers for microscope slides; FFPE tissue blocks can either be wrapped in paper or placed in a box.

• Formalin-fixed paraffin-embedded (FFPE) tissue block
• 1 H&E stained slide representative of the submitted tissue block.
• Histopathology report for the submitted tissue block

Not applicable

Indefinite

Not applicable

Not applicable

• Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.
• FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.
• Transport specimen at 15°C ~25 °C (room temperature)

• Incomplete information in the Request Form
• Improper or insufficient labeling of tissue blocks or slides
• Inadequate tissue available on the tissue block

Monday- Friday

The day before running day

10 working days except saturday, sundays and Holidays

Not applicable

Not applicable

Not applicable

Not applicable

IHC - Carcinoembryonic Antigen

Not applicable

The following CD markers are expressed on specific hemotologic cells. Individual and a series of IHC markers can be run on unknown cells and presence or absence of the antigen can help to characterize the cell of interest.

Immuno Histochemistry

Special Test

No patient preparation necessary.

Slide holders or mailers for microscope slides; FFPE tissue blocks can either be wrapped in paper or placed in a box.

• Formalin-fixed paraffin-embedded (FFPE) tissue block
• 1 H&E stained slide representative of the submitted tissue block.
• Histopathology report for the submitted tissue block

Not Applicable

Indefinite

Not applicable

Not applicable

• Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.
• FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.
• Transport specimen at 15°C ~25 °C (room temperature)

• Incomplete information in the Request Form
• Improper or insufficient labeling of tissue blocks or slides
• Inadequate tissue available on the tissue block

Tuesday and Thursday

Day before running day

10 days (excluding Saturdays, Sundays and Holidays)

Not Applicable

None specified.

Not applicable

CEA, Carcinoembryonic Antigen

IHC - Carcinoembryonic Antigen

N/A

The following CD markers are expressed on specific hemotologic cells. Individual and a series of IHC markers can be run on unknown cells and presence or absence of the antigen can help to characterize the cell of interest.

Immuno Histochemistry

Special Test

No patient preparation necessary.

Slide holders or mailers for microscope slides; FFPE tissue blocks can either be wrapped in paper or placed in a box.

• Formalin-fixed paraffin-embedded (FFPE) tissue block
• 1 H&E stained slide representative of the submitted tissue block.
• Histopathology report for the submitted tissue block

Not Applicable

Indefinite

Not applicable

Not applicable

• Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.
• FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.
• Transport specimen at 15°C ~25 °C (room temperature)

• Incomplete information in the Request Form
• Improper or insufficient labeling of tissue blocks or slides
• Inadequate tissue available on the tissue block

Tuesday and Thursday

Day before running day

10 days (excluding Saturdays, Sundays and Holidays)

Not Applicable

None specified.

Not applicable

CEA, Carcinoembryonic Antigen

• CYTOKERATIN 19
• CK-19

The use of Cytokeratin 19 (CK19) immunohistochemistry in lesions of the pancreas, gastrointestinal tract, and liver.

Immuno histochemistry

Special Test

No preparation needed

Slide holders or mailers for microscope slides; FFPE tissue blocks can either be wrapped in paper or placed in a box.

• Formalin-fixed paraffin-embedded (FFPE) tissue block
• 1 H&E stained slide representative of the submitted tissue block.
• Histopathology report for the submitted tissue block

Not Applicable

Indefinite

Not Applicable

Not Applicable

Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.
• FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.
• Transport specimen at 15°C ~25 °C (room temperature)

• Incomplete information in the Request Form
• Improper or insufficient labeling of tissue blocks or slides
• Inadequate tissue available on the tissue block

Tuesday, Thursday and Saturday

 A day before running day

7 working days excluding saturday, sunday and holidays

Not Applicable

Not Applicable

Not Applicable

Not Applicable

Cytokeratin 20

Not applicable

- Used in the identification of normal CK20 expressing cells and neoplastic cells of colonic epithelial lineage.

- Mucinous ovarian carcinomas, transitional cell carcinomas and Merkel cell carcinomas are also usually positive for CK20.

Immunohistochemistry (IHC)

Histopathology

All specimens must be LABELED properly and legibly. The label must match the information on the test request form and on the provided histopathology report.

- Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.

- FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.

- Formalin-fixed paraffin-embedded (FFPE) tissue block

- H&E stained slide, representative of the submitted tissue block.

- Histopathology report for the submitted tissue block and slide

- Completely filled out HPD Histopathology Request Form (HPD_F_258)

Note:Submit to HPD Main Laboratory all tissue blocks and slides received.

Not applicable

- FFPE tissue blocks and H&E slides are stable indefinitely when stored properly at room temperature (15^oC~30^oC).

- Hi-Precision Diagnostics will store FFPE tissue blocks and H&E slides for 10 years, after which samples will be disposed properly according to biosafety disposal guidelines.

Indefinite

Not applicable

Not applicable

Transport specimen at 15^oC -30^oC

- Incorrect/Incomplete information in the HPD Histopathology Request Form

- Improper or insufficient labeling of tissue blocks or slides

- Inadequate tissue available on the tissue block

- Tissue blocks and slides do not correspond to its histopathology report

- Specimen stored and transported outside the required temperature

Monday to Saturday

12:00 PM

- With Reading - 3 to 5 days after receipt of specimen/ messengers arrival or 10 working days (if with special test)

- For Processing Only - 1 day after receipt of specimen/ messengers arrival

Note: Additional 2 days may be necessary if specimen needs further evaluation and confirmation by another Pathologist

- The clinical interpretation of any staining, or absence of staining, must be complemented by histological studies and evaluation of proper controls.

- Evaluation must be made by a qualified pathologist within the context of the patient’s clinical history and other diagnostic tests.

- Ventana recommends fixation in 10% NBF for 6-48 hours.

- Fixation for less than 6 hours is not recommended.

Q. What is Immunohistochemistry?

A.Immunohistochemistry or IHC refers to the process of detecting antigens (e.g., proteins) in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues.

Q. Why do we need to undergo Immunohistochemistry test?

A. - Confirm a pathologic diagnosis

      - Differentiate between benign or malignant tumor

      - Detect among different types of tumor

      - Monitor prognosis response to treatment

- Immunohistochemistry (IHC)

- CK20

Cytokeratin 5/6

Not applicable

- Anti-Cytokeratin 5/6 (D5/16B4) Mouse Monoclonal Primary Antibody detects cytokeratins 5 and 6.

- Cytokeratin 5 is expressed in various epithelia and mesothelial cells, while cytokeratin 6 is expressed by proliferating squamous epithelium.

- This antibody exhibits a cytoplasmic staining pattern and may be used to aid in the identification of squamous cell carcinoma and in distinguishing malignant mesothelioma of the lung adenocarcinomas.

- The detection of cytokeratin 5 in myoepithelial cells with this antibody may also be used to aid in the determination of breast and prostate malignancies.

- Together with p63 , this antibody identify squamous origin in poorly differentiated metastatic carcinomas (e.g., cervical)

- As part of panel, this antibody discriminate between undifferentiated sinonasal carcinoma (CK5/6-) and other poorly differentiated head and neck tumors (poorly differentiated squamous cell carcinoma, olfactory neuroblastoma and nasopharyngeal carcinoma)

Immunohistochemistry (IHC)

Histopathology

All specimens must be LABELED properly and legibly. The label must match the information on the test request form and on the provided histopathology report.

- Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.

- FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.

- Formalin-fixed paraffin-embedded (FFPE) tissue block

- H&E stained slide, representative of the submitted tissue block.

- Histopathology report for the submitted tissue block and slide

- Completely filled out HPD Histopathology Request Form (HPD_F_258)

Note:Submit to HPD Main Laboratory all tissue blocks and slides received.

Not applicable

- FFPE tissue blocks and H&E slides are stable indefinitely when stored properly at room temperature (15^oC~30^oC).

- Hi-Precision Diagnostics will store FFPE tissue blocks and H&E slides for 10 years, after which samples will be disposed properly according to biosafety disposal guidelines.

Indefinite

Not applicable

Not applicable

Transport specimen at 15°C to 30°C

- Incorrect/Incomplete information in the HPD Histopathology Request Form

- Improper or insufficient labeling of tissue blocks or slides

- Inadequate tissue available on the tissue block

- Tissue blocks and slides do not correspond to its histopathology report

- Specimen stored and transported outside the required temperature

Monday to Saturday

12:00 PM

- With Reading - 3 to 5 days after receipt of specimen/ messengers arrival or 10 working days (if with special test)

- For Processing Only - 1 day after receipt of specimen/ messengers arrival

Note: Additional 2 days may be necessary if specimen needs further evaluation and confirmation by another Pathologist

- The clinical interpretation of any staining, or absence of staining, must be complemented by histological studies and evaluation of proper controls.

- Evaluation must be made by a qualified pathologist within the context of the patient’s clinical history and other diagnostic tests.

- Ventana recommends fixation in 10% NBF for 6-48 hours.

- Fixation for less than 6 hours is not recommended.

Q. What is Immunohistochemistry?

A.Immunohistochemistry or IHC refers to the process of detecting antigens (e.g., proteins) in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues.

Q. Why do we need to undergo Immunohistochemistry test?

A. - Confirm a pathologic diagnosis

      - Differentiate between benign or malignant tumor

      - Detect among different types of tumor

      - Monitor prognosis response to treatment

- Immunohistochemistry (IHC)

- Cytokeratin 5/6

- Cytokeratin

Cytokeratin 7

Not applicable

- Used in the identification of normal and neoplastic cells of the ovary, lung and breast epithelial cells which express CK7, and those of colonic and prostate epithelial cells which lack CK7 expression.

Immunohistochemistry (IHC)

Histopathology

All specimens must be LABELED properly and legibly. The label must match the information on the test request form and on the provided histopathology report.

- Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.

- FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.

- Formalin-fixed paraffin-embedded (FFPE) tissue block

- H&E stained slide, representative of the submitted tissue block.

- Histopathology report for the submitted tissue block and slide

- Completely filled out HPD Histopathology Request Form (HPD_F_258)

Note:Submit to HPD Main Laboratory all tissue blocks and slides received.

Not applicable

- FFPE tissue blocks and H&E slides are stable indefinitely when stored properly at room temperature (15^oC~30^oC).

- Hi-Precision Diagnostics will store FFPE tissue blocks and H&E slides for 10 years, after which samples will be disposed properly according to biosafety disposal guidelines.

Indefinite

Not applicable

Not applicable

Transport specimen at 15^oC -30^oC

- Incorrect/Incomplete information in the HPD Histopathology Request Form

- Improper or insufficient labeling of tissue blocks or slides

- Inadequate tissue available on the tissue block

- Tissue blocks and slides do not correspond to its histopathology report

- Specimen stored and transported outside the required temperature

Monday to Saturday

12:00 PM

- With Reading - 3 to 5 days after receipt of specimen/ messengers arrival or 10 working days (if with special test)

- For Processing Only - 1 day after receipt of specimen/ messengers arrival

Note: Additional 2 days may be necessary if specimen needs further evaluation and confirmation by another Pathologist

- The clinical interpretation of any staining, or absence of staining, must be complemented by histological studies and evaluation of proper controls.

- Evaluation must be made by a qualified pathologist within the context of the patient’s clinical history and other diagnostic tests.

- Ventana recommends fixation in 10% NBF for 6-48 hours.

- Fixation for less than 6 hours is not recommended.

Q. What is Immunohistochemistry?

A.Immunohistochemistry or IHC refers to the process of detecting antigens (e.g., proteins) in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues.

Q. Why do we need to undergo Immunohistochemistry test?

A. - Confirm a pathologic diagnosis

      - Differentiate between benign or malignant tumor

      - Detect among different types of tumor

      - Monitor prognosis response to treatment

- Immunohistochemistry (IHC)

- CK7

Not applicable

Not applicable

• Approximately 65% of mantle cell lymphomas (MCL) are characterized by a t (11; 14) (q13; Q32) gene translocation that causes deregulation of the expression of the BCL-1 gene. This abnormality in turn leads to deregulation of Cyclin D1. Detection of Cyclin D1 is useful in detecting disease.

Immuno Histochemistry

Special Test

No patient preparation necessary.

Slide holders or mailers for microscope slides; FFPE tissue blocks can either be wrapped in paper or placed in a box.

• Formalin-fixed paraffin-embedded (FFPE) tissue block
• 1 H&E stained slide representative of the submitted tissue block.
• Histopathology report for the submitted tissue block

Not Applicable

Indefinite

Not applicable

Not applicable

• Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.
• FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.
• Transport specimen at 15°C ~25 °C (room temperature)

• Incomplete information in the Request Form
• Improper or insufficient labeling of tissue blocks or slides
• Inadequate tissue available on the tissue block

Monday to Friday

Day before running day

10 days (excluding Saturdays, Sundays and Holidays)

Not Applicable

None specified.

Not applicable

- Cytokeratin

- AE1/AE3

Not applicable

- Confirmation of epithelial nature of tissue, tumors, or components of tumors.

- Detects keratin in all carcinomas, including poorly differentiated carcinomas.

Immunohistochemistry (IHC)

Histopathology

All specimens must be LABELED properly and legibly. The label must match the information on the test request form and on the provided histopathology report.

- Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.

- FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.

- Formalin-fixed paraffin-embedded (FFPE) tissue block

- H&E stained slide, representative of the submitted tissue block.

- Histopathology report for the submitted tissue block and slide

- Completely filled out HPD Histopathology Request Form (HPD_F_258)

Note:Submit to HPD Main Laboratory all tissue blocks and slides received.

Not applicable

- FFPE tissue blocks and H&E slides are stable indefinitely when stored properly at room temperature (15^oC~30^oC).

- Hi-Precision Diagnostics will store FFPE tissue blocks and H&E slides for 10 years, after which samples will be disposed properly according to biosafety disposal guidelines.

Indefinite

Not applicable

Not applicable

Transport specimen at 15°C to 30°C

- Incorrect/Incomplete information in the HPD Histopathology Request Form

- Improper or insufficient labeling of tissue blocks or slides

- Inadequate tissue available on the tissue block

- Tissue blocks and slides do not correspond to its histopathology report

- Specimen stored and transported outside the required temperature

Monday to Saturday

12:00 PM

- With Reading - 3 to 5 days after receipt of specimen/ messengers arrival or 10 working days (if with special test)

- For Processing Only - 1 day after receipt of specimen/ messengers arrival

Note: Additional 2 days may be necessary if specimen needs further evaluation and confirmation by another Pathologist

- The clinical interpretation of any staining, or absence of staining, must be complemented by histological studies and evaluation of proper controls.

- Evaluation must be made by a qualified pathologist within the context of the patient’s clinical history and other diagnostic tests.

- Ventana recommends fixation in 10% NBF for 6-48 hours.

- Fixation for less than 6 hours is not recommended.

Q. What is Immunohistochemistry?

A.Immunohistochemistry or IHC refers to the process of detecting antigens (e.g., proteins) in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues.

Q. Why do we need to undergo Immunohistochemistry test?

A. - Confirm a pathologic diagnosis

      - Differentiate between benign or malignant tumor

      - Detect among different types of tumor

      - Monitor prognosis response to treatment

- Immunohistochemistry (IHC)

- AE1/AE3

- Cytokeratin

Desmin

Not applicable

- Used to aid in the identification of cells of normal and abnormal myocytic lineage as an aid in diagnosis of anaplastic tumors.

- Desmin antibody reacts with Leiomyomas, Rhabdomyomas, and Perivascular cells of Glomus Tumors of the skin (if they are of myogenic nature), frequently used to identify tumor derivation.

Immunohistochemistry (IHC)

Histopathology

All specimens must be LABELED properly and legibly. The label must match the information on the test request form and on the provided histopathology report

- Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.

- FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.

- Formalin-fixed paraffin-embedded (FFPE) tissue block

- H&E stained slide, representative of the submitted tissue block.

- Histopathology report for the submitted tissue block and slide

- Completely filled out HPD Histopathology Request Form (HPD_F_258)

Note:Submit to HPD Main Laboratory all tissue blocks and slides received.

Not Applicable

- FFPE tissue blocks and H&E slides are stable indefinitely when stored properly at room temperature (15^oC~30^oC).

- Hi-Precision Diagnostics will store FFPE tissue blocks and H&E slides for 10 years, after which samples will be disposed properly according to biosafety disposal guidelines.

Indefinite

Not applicable

Not applicable

Transport specimen at 15^oC -30^oC

- Incorrect/Incomplete information in the HPD Histopathology Request Form

- Improper or insufficient labeling of tissue blocks or slides

- Inadequate tissue available on the tissue block

- Tissue blocks and slides do not correspond to its histopathology report

- Specimen stored and transported outside the required temperature

Monday to Saturday

12:00 PM

- With Reading - 3 to 5 days after receipt of specimen/ messengers arrival or 10 working days (if with special test)

- For Processing Only - 1 day after receipt of specimen/ messengers arrival

Note: Additional 2 days may be necessary if specimen needs further evaluation and confirmation by another Pathologist

- The clinical interpretation of any staining, or absence of staining, must be complemented by histological studies and evaluation of proper controls.

- Evaluation must be made by a qualified pathologist within the context of the patient’s clinical history and other diagnostic tests.

- Ventana recommends fixation in 10% NBF for 6-48 hours.

- Fixation for less than 6 hours is not recommended.

Q. What is Immunohistochemistry?

A.Immunohistochemistry or IHC refers to the process of detecting antigens (e.g., proteins) in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues.

Q. Why do we need to undergo Immunohistochemistry test?

A. - Confirm a pathologic diagnosis

      - Differentiate between benign or malignant tumor

      - Detect among different types of tumor

      - Monitor prognosis response to treatment

- Immunohistochemistry (IHC)

- DES

- Desmin