Laboratory Test Preparation

IHC - Mammaglobin

Not applicable

Mammaglobin, a very large complex glycoprotein, is identified in up to 85% of primary and metastatic breast carcinomas.

Special Test

Special Test

No patient preparation necessary.

Slide holders or mailers for microscope slides; FFPE tissue blocks can either be wrapped in paper or placed in a box.

• Formalin-fixed paraffin-embedded (FFPE) tissue block
• 1 H&E stained slide representative of the submitted tissue block.
• Histopathology report for the submitted tissue block

Not Applicable

Indefinite

Not applicable

Not applicable

• Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.
• FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.
• Transport specimen at 15°C ~25 °C (room temperature)

• Incomplete information in the Request Form
• Improper or insufficient labeling of tissue blocks or slides
• Inadequate tissue available on the tissue block

Monday to Friday

Day before running day

10 days (excluding Saturdays, Sundays and Holidays)

Not Applicable

None specified.

Not applicable

Not applicable

Melanin is normally found in the skin, eye, and substantia nigra. It may also be found in melanomas. The commonly used Fontana-Masson ("melanin stain") methodrelies upon the melanin granules to reduce ammoniacal silver nitrate (but argentaffin, chromaffin, and some lipochrome pigments also will stain black as well).

Immuno Histochemistry

Special Test

No patient preparation necessary.

Slide holders or mailers for microscope slides; FFPE tissue blocks can either be wrapped in paper or placed in a box.

• Formalin-fixed paraffin-embedded (FFPE) tissue block
• 1 H&E stained slide representative of the submitted tissue block.
• Histopathology report for the submitted tissue block

Not Applicable

Indefinite

Not applicable

Not applicable

• Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.
• FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.
• Transport specimen at 15°C ~25 °C (room temperature)

• Incomplete information in the Request Form
• Improper or insufficient labeling of tissue blocks or slides
• Inadequate tissue available on the tissue block

Tuesdays, Thursdays and Saturdays

Day before running day

10 days (excluding Saturdays, Sundays and Holidays)

Not Applicable

None specified.

Not applicable

Not applicable

Not applicable

• Identify skeletal muscle and smooth muscle cells in normal tissue or various disease entities
• Classify tumors with smooth or skeletal muscle, pericytes, myofibroblasts or myoepithelial cells
• Differentiate leiomyosarcoma (MSA , keratin-) from spindle cell carcinoma

Immuno Histochemistry

Special Test

No patient preparation necessary.

Slide holders or mailers for microscope slides; FFPE tissue blocks can either be wrapped in paper or placed in a box.

• Formalin-fixed paraffin-embedded (FFPE) tissue block
• 1 H&E stained slide representative of the submitted tissue block.
• Histopathology report for the submitted tissue block

Not Applicable

Indefinite

Not applicable

Not applicable

• Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.
• FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.
• Transport specimen at 15°C ~25 °C (room temperature)

• Incomplete information in the Request Form
• Improper or insufficient labeling of tissue blocks or slides
• Inadequate tissue available on the tissue block

Mondays, Wednesdays and Fridays

Day before running day

10 days (excluding Saturdays, Sundays and Holidays)

Not Applicable

None specified.

Not applicable

SMA, Anti-Smooth Muscle Antibody, ASMA; Actin Antibody, F-Actin Antibody, ACTA, Smooth Muscle Antibody

IHC- MPO

Not applicable

This marker can be used to aid in the diagnosis and/or prognosis of cancer. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Immuno Histochemistry

Special Test

No patient preparation necessary.

Slide holders or mailers for microscope slides; FFPE tissue blocks can either be wrapped in paper or placed in a box.

• Formalin-fixed paraffin-embedded (FFPE) tissue block
• 1 H&E stained slide representative of the submitted tissue block.
• Histopathology report for the submitted tissue block

Not Applicable

Indefinite

Not applicable

Not applicable

• Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.
• FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.
• Transport specimen at 15°C ~25 °C (room temperature)

• Incomplete information in the Request Form
• Improper or insufficient labeling of tissue blocks or slides
• Inadequate tissue available on the tissue block

Mondays, Wednesdays and Fridays

10:00 AM

After 1 week (excluding Saturdays, Sundays and Holidays)

Not Applicable

None specified.

Not Applicable

ANCA Antibodies, c-ANCA, p-ANCA, Serine Protease 3, PR3, Anticytoplasmic Autoantibodies, 3-ANCA, PR3-ANCA, MPO-ANCA, Antineutrophil Cytoplasmic Antibodies, Myeloperoxidase Antibodies, Proteinase 3 Antibodies

•  Myogenic differentiation 1 antibody

• Myogenic factor 3 antibody

• Myogenic factor MYF 3 antibody

• PUM antibody

Not applicable

• MyoD1 belongs to a family of proteins known as myogenic regulatory factors (MRFs) and has a key role in regulating muscle differentation. These bHLH (basic helix loop helix) transcription factors act sequentially in myogenic differentiation. 

• MyoD1 commits mesoderm cells to a skeletal lineage, and then regulates that process. It may also play a role in muscle repair.
In abnormal tissues, MyoD1 labels tumor cells in Rhabdomyosarcoma and is one of the earliest markers of myogenic commitment.

Immuno Histochemistry

Special Test

No patient preparation necessary.

Slide holders or mailers for microscope slides; FFPE tissue blocks can either be wrapped in paper or placed in a box.

• Formalin-fixed paraffin-embedded (FFPE) tissue block
• 1 H&E stained slide representative of the submitted tissue block.
• Histopathology report for the submitted tissue block

Not Applicable

Indefinite

Not applicable

Not applicable

• Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.
• FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.
• Transport specimen at 15°C ~25 °C (room temperature)

• Incomplete information in the Request Form
• Improper or insufficient labeling of tissue blocks or slides
• Inadequate tissue available on the tissue block

Mondays, Wednesdays and Fridays

Day before running day

10 days (excluding Saturdays, Sundays and Holidays)

Not Applicable

None specified.

Not applicable

• MYF4

• bHLHc3

Not applicable

• Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture.

• It is a member of a large family of proteins related by sequence homology, the helix-loop-helix (HLH) proteins. It is essential for the development of functional skeletal muscle.

Immuno Histochemistry

Special Test

No patient preparation necessary.

Slide holders or mailers for microscope slides; FFPE tissue blocks can either be wrapped in paper or placed in a box.

• Formalin-fixed paraffin-embedded (FFPE) tissue block
• 1 H&E stained slide representative of the submitted tissue block.
• Histopathology report for the submitted tissue block

Not Applicable

Indefinite

Not applicable

Not applicable

• Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.
• FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.
• Transport specimen at 15°C ~25 °C (room temperature)

• Incomplete information in the Request Form
• Improper or insufficient labeling of tissue blocks or slides
• Inadequate tissue available on the tissue block

Tuesdays, Thursdays and Saturdays

Day before running day

10 days (excluding Saturdays, Sundays and Holidays)

Not Applicable

None specified.

Not applicable

Not Applicable

Not Applicable

Napsin A is a mouse monoclonal antibody that is
intended for laboratory use in the qualitative identification of Napsin A
protein by immunohistochemistry (IHC) in formalin-fixed paraffinembedded
(FFPE) human tissues.

Immunohistochem

Special Test

No patient Preparation

Slide holders or mailers for microscope slides; FFPE tissue blocks can either be wrapped in paper or placed in a box.

•  Formalin-fixed paraffin-embedded (FFPE) tissue block
• 1 H&E stained slide representative of the submitted tissue block.Histopathology report for the submitted tissue block
• Doctors order

Not Applicable

Indefinite

Not Applicable

Not Applicable

Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.
• FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.
• Transport specimen at 15°C ~25 °C (room temperature)

• Incomplete information in the Request Form
• Improper or insufficient labeling of tissue blocks or slides
• Inadequate tissue available on the tissue block

Tuesday, thursday and Saturday

Day before running day

10 days (excluding Saturdays, Sundays and Holidays)

Not Applicable

Not Specified

Not Applicable

Not Applicable

• IHC - Neuron Specific Enolase

Not applicable

• This marker can be used to aid in the diagnosis and/or prognosis of cancer. 

• NSE is increased in bronchopulmonary cancer, more specifically small cell bronchial cancer and neuroblastoma. Prognostic and therapeutic follow-up marker.

Immuno Histochemistry

Special Test

No patient preparation necessary.

Slide holders or mailers for microscope slides; FFPE tissue blocks can either be wrapped in paper or placed in a box.

• Formalin-fixed paraffin-embedded (FFPE) tissue block
• 1 H&E stained slide representative of the submitted tissue block.
• Histopathology report for the submitted tissue block

Not Applicable

Indefinite

Not applicable

Not applicable

• Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.
• FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.
• Transport specimen at 15°C ~25 °C (room temperature)

• Incomplete information in the Request Form
• Improper or insufficient labeling of tissue blocks or slides
• Inadequate tissue available on the tissue block

Tuesday, Thursday and Saturday

Day before running day

10 days (excluding Saturdays, Sundays and Holidays)

Not Applicable

None specified.

Not applicable

Not applicable 

Not applicable 

Immuno Histochemistry

Special Test

No patient preparation necessary

Slide holders or mailers for microscope slides; FFPE tissue blocks can either be wrapped in paper or placed in a box.

• Formalin-fixed paraffin-embedded (FFPE) tissue block
• 1 H&E stained slide representative of the submitted tissue block.
• Histopathology report for the submitted tissue block

Not applicable 

Indefinite

Not applicable 

Not applicable 

• Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.
• FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.
• Transport specimen at 15°C ~25 °C (room temperature)

• Incomplete information in the Request Form
• Improper or insufficient labeling of tissue blocks or slides
• Inadequate tissue available on the tissue block

Tuesday and Thursday

7am Day before running day 

10 days (excluding Saturdays, Sundays and Holidays)

Not applicable 

Not applicable 

Not applicable 

Not applicable 

IHC- P53

Not applicable

The result of this immunohistochemical study is reported as a percent of tumor nuclei that demonstrate moderate or strong immunoreactivity. Various published studies have suggested that overexpression of p53 protein in breast cancer may be an unfavorable prognostic indicator. For other types of tumors, definitive interpretive criteria for p53 expression may vary.

Immuno Histochemistry

Special Test

No patient preparation necessary.

Slide holders or mailers for microscope slides; FFPE tissue blocks can either be wrapped in paper or placed in a box.

• Formalin-fixed paraffin-embedded (FFPE) tissue block
• 1 H&E stained slide representative of the submitted tissue block.
• Histopathology report for the submitted tissue block

Not Applicable

Indefinite

Not applicable

Not applicable

• Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.
• FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.
• Transport specimen at 15°C ~25 °C (room temperature)

• Incomplete information in the Request Form
• Improper or insufficient labeling of tissue blocks or slides
• Inadequate tissue available on the tissue block

Monday, Wednesday and Friday

Day before running day

10 days (excluding Saturdays, Sundays and Holidays)

Not Applicable

None specified.

Not applicable

Tumor protein

- p63

-Transformation-related protein 63 (TP63)

Not applicable

- Identification of myoepithelial cells in sclerosing breast lesions.

- Diagnosis of prostatic carcinoma by showing absence of basal cells.

- Differentiation of basaloid squamous lung cancer ( ) vs. small cell (-).

- Identification of metastatic poorly differentiated squamous cell carcinoma.

Immunohistochemistry (IHC)

Histopathology

All specimens must be LABELED properly and legibly. The label must match the information on the test request form and on the provided histopathology report.

- Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.

- FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.

- Formalin-fixed paraffin-embedded (FFPE) tissue block

- H&E stained slide, representative of the submitted tissue block.

- Histopathology report for the submitted tissue block and slide

- Completely filled out HPD Histopathology Request Form (HPD_F_258)

Note:Submit to HPD Main Laboratory all tissue blocks and slides received.

Not applicable

- FFPE tissue blocks and H&E slides are stable indefinitely when stored properly at room temperature (15^oC~30^oC).

- Hi-Precision Diagnostics will store FFPE tissue blocks and H&E slides for 10 years, after which samples will be disposed properly according to biosafety disposal guidelines.

Indefinite

Not applicable

Not applicable

Transport specimen at 15°C to 30°C

- Incorrect/Incomplete information in the HPD Histopathology Request Form

- Improper or insufficient labeling of tissue blocks or slides

- Inadequate tissue available on the tissue block

- Tissue blocks and slides do not correspond to its histopathology report

- Specimen stored and transported outside the required temperature

Monday to Saturday

12:00 PM

- With Reading - 3 to 5 days after receipt of specimen/ messengers arrival or 10 working days (if with special test)

- For Processing Only - 1 day after receipt of specimen/ messengers arrival

Note: Additional 2 days may be necessary if specimen needs further evaluation and confirmation by another Pathologist

- The clinical interpretation of any staining, or absence of staining, must be complemented by histological studies and evaluation of proper controls.

- Evaluation must be made by a qualified pathologist within the context of the patient’s clinical history and other diagnostic tests.

- Ventana recommends fixation in 10% NBF for 6-48 hours.

- Fixation for less than 6 hours is not recommended.

Q. What is Immunohistochemistry?

A.Immunohistochemistry or IHC refers to the process of detecting antigens (e.g., proteins) in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues.

Q. Why do we need to undergo Immunohistochemistry test?

A. - Confirm a pathologic diagnosis

      - Differentiate between benign or malignant tumor

      - Detect among different types of tumor

      - Monitor prognosis response to treatment

- Immunohistochemistry (IHC)

- p63

-Transformation-related protein 63 (TP63)

Periodic acid–Schiff

Not Applicable

for detection of glycogen in tissues

Special Staining

Special test

No patient preparation necessary.

Slide holders or mailers for microscope slides; FFPE tissue blocks can either be wrapped in paper or placed in a box.

• Formalin-fixed paraffin-embedded (FFPE) tissue block
• 1 H&E stained slide representative of the submitted tissue block.
• Histopathology report for the submitted tissue block

Not Applicable

Indefinite

Not Applicable

Not Applicable

Transport specimen at 15°C ~25 °C (room temperature)

• Incomplete information in the Request Form
• Improper or insufficient labeling of tissue blocks or slides
• Inadequate tissue available on the tissue block

Tuesday and Thursday

Monday and Wednesday 7am

10 working days except saturday, sunday and Holidays.

Not Applicable

Not Applicable

Not Applicable

Not applicable

Not applicable

• Pax genes are a family of developmental control genes that encode nuclear transcription factors and have been implicated in the control of mammalian development. PAX-5 is a B cell speciric transcription factor that is expressed in pro B-cells, pre-B and mature B cells, and subsequently in all stages of B cell development until that plasma cell stage in which it is down regulated.

Immuno Histochemistry

Special Test

No patient preparation necessary.

Slide holders or mailers for microscope slides; FFPE tissue blocks can either be wrapped in paper or placed in a box.

• Formalin-fixed paraffin-embedded (FFPE) tissue block
• 1 H&E stained slide representative of the submitted tissue block.
• Histopathology report for the submitted tissue block

Not applicable

Indefinite

Not applicable

Not applicable

• Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.
• FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.
• Transport specimen at 15°C ~25 °C (room temperature)

• Incomplete information in the Request Form
• Improper or insufficient labeling of tissue blocks or slides
• Inadequate tissue available on the tissue block

Tuesday and Thursday

1 day before running day

7 days (Excluding Saturdays, Sundays and Holidays)

Not Applicable

None specified.

Not applicable

Not applicable

To Expressed in a high percentage of renal cell carcinomas and ovarian cancers. 

Immuno Histochemistry

Special Test 

No patient preparation necessary.

Slide holders or mailers for microscope slides; FFPE tissue blocks can either be wrapped in paper or placed in a box

• Formalin-fixed paraffin-embedded (FFPE) tissue block
• 1 H&E stained slide representative of the submitted tissue block.
• Histopathology report for the submitted tissue block

Not applicable

Indefinite

Not applicable

Not applicable

• Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.
• FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.
• Transport specimen at 15°C ~25 °C (room temperature)

• Incomplete information in the Request Form
• Improper or insufficient labeling of tissue blocks or slides
• Inadequate tissue available on the tissue block

Tuesday and Thursday

1 day before running day

7 days (Excluding Saturdays, Sundays and Holidays)

Not applicable

Not applicable

Not applicable

Not applicable

• IHC - PDL-1 (Nivolumab)

Not applicable

The PD-L1 IHC 28-8pharmaDx qualitative assay is intended for use in the detection of PD-L1 expression in formalin-fixed, paraffin embedded (FFPE) non-squamous non-small cell lung carcinoma (NSCLC). PD-L1 expression in non-squamous NSCLC may be associated with enhanced survival from OPDIVO (nivolumab).

Immuno Histochemistry

Special Test

No patient preparation necessary.

Slide holders or mailers for microscope slides; FFPE tissue blocks can either be wrapped in paper or placed in a box.

• Formalin-fixed paraffin-embedded (FFPE) tissue block
• 1 H&E stained slide representative of the submitted tissue block.
• Histopathology report for the submitted tissue block

Not Applicable

Indefinite

Not applicable

Not applicable

• Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.
• FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.
• Transport specimen at 15°C ~25 °C (room temperature)

• Incomplete information in the Request Form
• Improper or insufficient labeling of tissue blocks or slides
• Inadequate tissue available on the tissue block

Batch running

Monday to Friday,12:00 PM

14 days after cut-off (excluding Saturdays, Sundays and Holidays)

Not Applicable

None specified.

Not applicable