Laboratory Test Preparation

| Test | : | IHC-DOG1 |
| Other Test Request Name | : | Not applicable |
| Test Composition | : | Not applicable |
| Intended Use | : | • Protein of unknown function which is selectively expressed in gastrointestinal stromal tumour. |
| Methodology | : | Immuno Histochemistry |
| Laboratory Section | : | Special Test |
| Special Instructions/Patient Preparations | : | Not applicable |
| Collection/Sample Container | : | Slide holders or mailers for microscope slides; FFPE tissue blocks can either be wrapped in paper or placed in a box. |
| Specimen and Volume Requirement Note:Follow tube manufacturer recommendation. | : | • Formalin-fixed paraffin-embedded (FFPE) tissue block |
| Alternative Specimen and Volume Requirement | : | Not Applicable |
| Specimen Stability | ||
| • Room Temperature (15-25°C) | : | Indefinite |
| • Refrigerated Temperature (2-8°C) | : | Not applicable |
| • Freezer Temperature (-20°C) | : | Not applicable |
| Transport Temperature | : | • Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage. |
| Rejection Criteria | : | • Incomplete information in the Request Form |
| Running Day | : | Monday to Friday |
| Cut Off Time | : | 10 days (excluding Saturdays, Sundays and Holidays) |
| TAT/Releasing of Results | : | 10 days (excluding Saturdays, Sundays and Holidays) |
| Reference Interval/Result Interpretation | : | Not Applicable |
| Limitations/Interferences | : | None specified. |
| Frequently Asked Questions (FAQs) | : | Not applicable |
| Related Words/Test | : | Not Applicable |
| Test | : | IHC-ER/PR |
| Other Test Request Name | : | - Estrogen Receptor - Progesterone Receptor |
| Test Composition | : | Not applicable |
| Intended Use | : | ER/PR Assay (BREAST CA)
ER/PR Assay (OTHERS)
|
| Methodology | : | Immunohistochemistry (IHC) |
| Laboratory Section | : | Histopathology |
| Special Instructions/Patient Preparations | : | No patient preparation needed |
| Collection/Sample Container | : | - Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage. - FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping. |
| Specimen and Volume Requirement Note:Follow tube manufacturer recommendation. | : | - Formalin-fixed paraffin-embedded (FFPE) tissue block - H&E stained slide, representative of the submitted tissue block. - Histopathology report for the submitted tissue block and slide - Completely filled out HPD Histopathology Request Form (HPD_F_258) Note:Submit to HPD Main Laboratory all tissue blocks and slides received. |
| Alternative Specimen and Volume Requirement | : | Not applicable |
| Specimen Stability | - FFPE tissue blocks and H&E slides are stable indefinitely when stored properly at room temperature (15oC~30oC). - Hi-Precision Diagnostics will store FFPE tissue blocks and H&E slides for 10 years, after which samples will be disposed properly according to biosafety disposal guidelines. | |
| • Room Temperature (15-25°C) | : | Indefinite |
| • Refrigerated Temperature (2-8°C) | : | Not applicable |
| • Freezer Temperature (-20°C) | : | Not applicable |
| Transport Temperature | : | Transport specimen at 15oC -30oC |
| Rejection Criteria | : | - Incorrect/Incomplete information in the HPD Histopathology Request Form - Improper or insufficient labeling of tissue blocks or slides - Inadequate tissue available on the tissue block - Tissue blocks and slides do not correspond to its histopathology report - Specimen stored and transported outside the required temperature |
| Running Day | : | Monday to Saturday |
| Cut Off Time | : | 12:00 PM |
| TAT/Releasing of Results | : | - With Reading - 3 to 5 days after receipt of specimen/ messengers arrival or 10 working days (if with special test) - For Processing Only - 1 day after receipt of specimen/ messengers arrival Note: Additional 2 days may be necessary if specimen needs further evaluation and confirmation by another Pathologist |
| Reference Interval/Result Interpretation | : | - The clinical interpretation of any staining, or absence of staining, must be complemented by histological studies and evaluation of proper controls. - Evaluation must be made by a qualified pathologist within the context of the patient’s clinical history and other diagnostic tests. |
| Limitations/Interferences | : | - Ventana recommends fixation in 10% NBF for 6-72 hours. - Fixation for less than 6 hours is not recommended. - The use of Bouin’s, and alcohol fixatives, such as AFA and 95% Ethanol, is not recommended due to weaker staining. - Cold ischemic time over 1 hour |
| Frequently Asked Questions (FAQs) | : | Q. What is Immunohistochemistry? A.Immunohistochemistry or IHC refers to the process of detecting antigens (e.g., proteins) in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues. Q. Why do we need to undergo Immunohistochemistry test? A. - Confirm a pathologic diagnosis - Differentiate between benign or malignant tumor - Detect among different types of tumor - Monitor prognosis response to treatment |
| Related Words/Test | : | - Immunohistochemistry (IHC) - Breast Cancer - Estrogen Receptor - Progesterone Receptor
|
| Test | : | IHC-ERCC1 |
| Other Test Request Name | : | • Excision Repair Cross Complementing 1 • UV20 • COFS4 • RAD10
|
| Test Composition | : | Not applicable |
| Intended Use | : | Increased ERCC1 expression has been shown to predict cisplatin resistance and therefore decreased survival in gastric, ovarian, esophageal, colorectal cancers, and NSCLC. This is consistent with the role of ERCC1 in the repair of modified nucleotides, specifically increased removal of cisplatinum-induced DNA adducts. |
| Methodology | : | Immuno Histochemistry |
| Laboratory Section | : | Special Test |
| Special Instructions/Patient Preparations | : | No patient preparation necessary. |
| Collection/Sample Container | : | Slide holders or mailers for microscope slides; FFPE tissue blocks can either be wrapped in paper or placed in a box. |
| Specimen and Volume Requirement Note:Follow tube manufacturer recommendation. | : | • Formalin-fixed paraffin-embedded (FFPE) tissue block |
| Alternative Specimen and Volume Requirement | : | Not applicable |
| Specimen Stability | ||
| • Room Temperature (15-25°C) | : | Indefinite |
| • Refrigerated Temperature (2-8°C) | : | Not applicable |
| • Freezer Temperature (-20°C) | : | Not applicable |
| Transport Temperature | : | • Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage. |
| Rejection Criteria | : | • Incomplete information in the Request Form |
| Running Day | : | Batch running |
| Cut Off Time | : | Monday, 12:00 PM
|
| TAT/Releasing of Results | : | 2 weeks after cut-off (excluding Saturdays, Sundays and Holidays) |
| Reference Interval/Result Interpretation | : | Not Applicable |
| Limitations/Interferences | : | None specified. |
| Frequently Asked Questions (FAQs) | : | Not applicable |
| Related Words/Test | : | Not Applicable |
| Test | : | IHC-ERG |
| Other Test Request Name | : | Not Applicable |
| Test Composition | : | Not Applicable |
| Intended Use | : | Not Applicable |
| Methodology | : | Immuno Histochemistry |
| Laboratory Section | : | Special Test |
| Special Instructions/Patient Preparations | : | No patient preparation necessary. |
| Collection/Sample Container | : | Slide holders or mailers for microscope slides; FFPE tissue blocks can either be wrapped in paper or placed in a box. |
| Specimen and Volume Requirement Note:Follow tube manufacturer recommendation. | : | Formalin-fixed paraffin-embedded (FFPE) tissue block |
| Alternative Specimen and Volume Requirement | : | Not Applicable |
| Specimen Stability | ||
| • Room Temperature (15-25°C) | : | Indefinite |
| • Refrigerated Temperature (2-8°C) | : | Not Applicable |
| • Freezer Temperature (-20°C) | : | Not Applicable |
| Transport Temperature | : | Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage. |
| Rejection Criteria | : | • Incomplete information in the Request Form |
| Running Day | : | Tuesday, Thursday and Saturday |
| Cut Off Time | : | A day before running day |
| TAT/Releasing of Results | : | 10 days (excluding Saturdays, Sundays and Holidays) |
| Reference Interval/Result Interpretation | : | Not Applicable |
| Limitations/Interferences | : | Not Applicable |
| Frequently Asked Questions (FAQs) | : | Not Applicable |
| Related Words/Test | : | Not Applicable |
| Test | : | IHC-GATA3 Temporarily Unavailable (currently not accepting samples) |
| Other Test Request Name | : | Not Applicable |
| Test Composition | : | Not Applicable |
| Intended Use | : | Not Applicable |
| Methodology | : | Immuno Histochemistry
|
| Laboratory Section | : | Special Test |
| Special Instructions/Patient Preparations | : | No patient preparation necessary. |
| Collection/Sample Container | : | Slide holders or mailers for microscope slides; FFPE tissue blocks can either be wrapped in paper or placed in a box. |
| Specimen and Volume Requirement Note:Follow tube manufacturer recommendation. | : | • Formalin-fixed paraffin-embedded (FFPE) tissue block |
| Alternative Specimen and Volume Requirement | : | Not Applicable |
| Specimen Stability | ||
| • Room Temperature (15-25°C) | : | Indefinite |
| • Refrigerated Temperature (2-8°C) | : | Not Applicable |
| • Freezer Temperature (-20°C) | : | Not Applicable |
| Transport Temperature | : | • Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage. |
| Rejection Criteria | : | • Incomplete information in the Request Form |
| Running Day | : | Tuesday, Thursday and Saturday |
| Cut Off Time | : | Day before running day |
| TAT/Releasing of Results | : | 7 working days except saturday, sunday and Holidays |
| Reference Interval/Result Interpretation | : | Not Applicable |
| Limitations/Interferences | : | Not Applicable |
| Frequently Asked Questions (FAQs) | : | Not Applicable |
| Related Words/Test | : | Not Applicable |
| Test | : | IHC-GMS |
| Other Test Request Name | : | Grocott-Gomori's Methenamine Silver
|
| Test Composition | : | Not Applicable |
| Intended Use | : | Screen for fungal organism. Particularly useful in staining carbohydrates. |
| Methodology | : | Special Staining |
| Laboratory Section | : | Special test |
| Special Instructions/Patient Preparations | : | No patient preparation necessary. |
| Collection/Sample Container | : | Slide holders or mailers for microscope slides; FFPE tissue blocks can either be wrapped in paper or placed in a box |
| Specimen and Volume Requirement Note:Follow tube manufacturer recommendation. | : | • Formalin-fixed paraffin-embedded (FFPE) tissue block |
| Alternative Specimen and Volume Requirement | : | Not Applicable |
| Specimen Stability | ||
| • Room Temperature (15-25°C) | : | Indefinite |
| • Refrigerated Temperature (2-8°C) | : | Not Applicable |
| • Freezer Temperature (-20°C) | : | Not Applicable |
| Transport Temperature | : | Transport specimen at 15°C ~25 °C (room temperature) |
| Rejection Criteria | : |
|
| Running Day | : | Tuesday and Thursday |
| Cut Off Time | : | Monday and Wednesday |
| TAT/Releasing of Results | : | 10 working days excluding saturday, sunday and holidays |
| Reference Interval/Result Interpretation | : | Not Applicable |
| Limitations/Interferences | : | Not Applicable |
| Frequently Asked Questions (FAQs) | : | Not Applicable |
| Related Words/Test | : | Not Applicable |
| Test | : | IHC-HBME1 |
| Other Test Request Name | : | Not applicable |
| Test Composition | : | Not applicable |
| Intended Use | : | Abnormally expressed in thyroid cancer, showing cytoplasmic location with membrane accentuation. |
| Methodology | : | Immuno Histochemistry |
| Laboratory Section | : | Special Test |
| Special Instructions/Patient Preparations | : | No patient preparation necessary. |
| Collection/Sample Container | : | Slide holders or mailers for microscope slides; FFPE tissue blocks can either be wrapped in paper or placed in a box. |
| Specimen and Volume Requirement Note:Follow tube manufacturer recommendation. | : | • Formalin-fixed paraffin-embedded (FFPE) tissue block |
| Alternative Specimen and Volume Requirement | : | Not Applicable |
| Specimen Stability | ||
| • Room Temperature (15-25°C) | : | Indefinite |
| • Refrigerated Temperature (2-8°C) | : | Not applicable |
| • Freezer Temperature (-20°C) | : | Not applicable |
| Transport Temperature | : | • Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage. |
| Rejection Criteria | : | • Incomplete information in the Request Form |
| Running Day | : | Tuesday, Thursday and Saturday |
| Cut Off Time | : | Day before running day |
| TAT/Releasing of Results | : | 10 days (excluding Saturdays, Sundays and Holidays) |
| Reference Interval/Result Interpretation | : | Not Applicable |
| Limitations/Interferences | : | None specified. |
| Frequently Asked Questions (FAQs) | : | Not Applicable |
| Related Words/Test | : | Not Applicable |
| Test | : | IHC-HEP-PAR |
| Other Test Request Name | : |
|
| Test Composition | : | Not applicable |
| Intended Use | : | • Hep par-1 is a reliable immunohistochemical marker for cases of hepatocellular carcinoma (HCC). It can be used along with other markers in morphologically difficult cases when differential diagnosis lies between poorly differentiated HCC and metastatic carcinoma of liver. |
| Methodology | : | Immuno Histochemistry |
| Laboratory Section | : | Special Test |
| Special Instructions/Patient Preparations | : | No patient preparation necessary. |
| Collection/Sample Container | : | Slide holders or mailers for microscope slides; FFPE tissue blocks can either be wrapped in paper or placed in a box. |
| Specimen and Volume Requirement Note:Follow tube manufacturer recommendation. | : | • Formalin-fixed paraffin-embedded (FFPE) tissue block |
| Alternative Specimen and Volume Requirement | : | Not Applicable |
| Specimen Stability | ||
| • Room Temperature (15-25°C) | : | Indefinite |
| • Refrigerated Temperature (2-8°C) | : | Not Applicable |
| • Freezer Temperature (-20°C) | : | Not Applicable |
| Transport Temperature | : | • Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage. |
| Rejection Criteria | : | • Incomplete information in the Request Form |
| Running Day | : | Mondays, Wednesdays and Fridays |
| Cut Off Time | : | Day before running day |
| TAT/Releasing of Results | : | 10 days (excluding Saturdays, Sundays and Holidays) |
| Reference Interval/Result Interpretation | : | Not Applicable |
| Limitations/Interferences | : | None specified. |
| Frequently Asked Questions (FAQs) | : | Not Applicable |
| Related Words/Test | : | Not Applicable |
| Test | : | IHC-HER-2 NEU |
| Other Test Request Name | : | - Her-2-neu - C-erbB-2 - Human epidemal growth factor receptor-2/neu |
| Test Composition | : | Not applicable |
| Intended Use | : | HER-2 NEU (BREAST CA)
HER-2 NEU (OTHERS)
|
| Methodology | : | Immunohistochemistry (IHC) |
| Laboratory Section | : | Histopathology |
| Special Instructions/Patient Preparations | : | No patient preparation needed |
| Collection/Sample Container | : | - Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage. - FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping. |
| Specimen and Volume Requirement Note:Follow tube manufacturer recommendation. | : | - Formalin-fixed paraffin-embedded (FFPE) tissue block - H&E stained slide, representative of the submitted tissue block. - Histopathology report for the submitted tissue block and slide - Completely filled out HPD Histopathology Request Form (HPD_F_258) Note:Submit to HPD Main Laboratory all tissue blocks and slides received. |
| Alternative Specimen and Volume Requirement | : | Not applicable |
| Specimen Stability | - FFPE tissue blocks and H&E slides are stable indefinitely when stored properly at room temperature (15oC~30oC). - Hi-Precision Diagnostics will store FFPE tissue blocks and H&E slides for 10 years, after which samples will be disposed properly according to biosafety disposal guidelines. | |
| • Room Temperature (15-25°C) | : | Indefinite |
| • Refrigerated Temperature (2-8°C) | : | Not applicable |
| • Freezer Temperature (-20°C) | : | Not applicable |
| Transport Temperature | : | Transport specimen at 15°C to 30°C |
| Rejection Criteria | : | - Incorrect/Incomplete information in the HPD Histopathology Request Form - Improper or insufficient labeling of tissue blocks or slides - Inadequate tissue available on the tissue block - Tissue blocks and slides do not correspond to its histopathology report - Specimen stored and transported outside the required temperature |
| Running Day | : | Monday to Saturday |
| Cut Off Time | : | 12:00 PM |
| TAT/Releasing of Results | : | - With Reading - 3 to 5 days after receipt of specimen/ messengers arrival or 10 working days (if with special test) - For Processing Only - 1 day after receipt of specimen/ messengers arrival Note: Additional 2 days may be necessary if specimen needs further evaluation and confirmation by another Pathologist |
| Reference Interval/Result Interpretation | : | - The clinical interpretation of any staining, or absence of staining, must be complemented by histological studies and evaluation of proper controls. - Evaluation must be made by a qualified pathologist within the context of the patient’s clinical history and other diagnostic tests. |
| Limitations/Interferences | : | - Ventana recommends fixation in 10% NBF for 6-72 hours. - Fixation for less than 6 hours is not recommended. - The use of Bouin’s, and alcohol fixatives, such as AFA and 95% Ethanol, is not recommended due to weaker staining. - Cold ischemic time over 1 hour |
| Frequently Asked Questions (FAQs) | : | Q. What is Immunohistochemistry? A.Immunohistochemistry or IHC refers to the process of detecting antigens (e.g., proteins) in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues. Q. Why do we need to undergo Immunohistochemistry test? A. - Confirm a pathologic diagnosis - Differentiate between benign or malignant tumor - Detect among different types of tumor - Monitor prognosis response to treatment |
| Related Words/Test | : | - Immunohistochemistry (IHC) - Breast Cancer - Her-2-neu - C-erbB-2 - Human epidemal growth factor receptor-2/neu |
| Test | : | IHC-HMB45 |
| Other Test Request Name | : | • IHC - Human Melanoma Black 45 |
| Test Composition | : | Not applicable |
| Intended Use | : | A monoclonal antibody that reacts against an antigen present in melanocytic tumors such as melanomas, and stands for human melanoma black 45. It is used in anatomic pathology as a marker for such tumors. The specific antigen recognized by HMB-45 is now known as Pmel 17. |
| Methodology | : | Immuno Histochemistry |
| Laboratory Section | : | Special Test |
| Special Instructions/Patient Preparations | : | No patient preparation necessary. |
| Collection/Sample Container | : | Slide holders or mailers for microscope slides; FFPE tissue blocks can either be wrapped in paper or placed in a box. |
| Specimen and Volume Requirement Note:Follow tube manufacturer recommendation. | : | • Formalin-fixed paraffin-embedded (FFPE) tissue block |
| Alternative Specimen and Volume Requirement | : | Not Applicable |
| Specimen Stability | ||
| • Room Temperature (15-25°C) | : | Indefinite |
| • Refrigerated Temperature (2-8°C) | : | Not applicable |
| • Freezer Temperature (-20°C) | : | Not applicable |
| Transport Temperature | : | • Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage. |
| Rejection Criteria | : | • Incomplete information in the Request Form |
| Running Day | : | Monday, Wednesday and Friday |
| Cut Off Time | : | Day before running day |
| TAT/Releasing of Results | : | 10 days (excluding Saturdays, Sundays and Holidays) |
| Reference Interval/Result Interpretation | : | Not Applicable |
| Limitations/Interferences | : | None specified. |
| Frequently Asked Questions (FAQs) | : | Not applicable |
| Related Words/Test | : | Melanocytic tumors |
| Test | : | IHC-HMWCK (34BE-12) |
| Other Test Request Name | : |
|
| Test Composition | : | Not Applicable
|
| Intended Use | : | A sensitive marker for differentiation of high-grade invasive urothelial carcinoma from prostate cancer. |
| Methodology | : | Immunohistochemistry (IHC) |
| Laboratory Section | : | Special Test |
| Special Instructions/Patient Preparations | : |
|
| Collection/Sample Container | : |
|
| Specimen and Volume Requirement Note:Follow tube manufacturer recommendation. | : |
Note:Submit to HPD Main Laboratory all tissue blocks and slides received.
|
| Alternative Specimen and Volume Requirement | : | Not Applicable
|
| Specimen Stability | ||
| • Room Temperature (15-25°C) | : | Indefinite |
| • Refrigerated Temperature (2-8°C) | : | Indefinite |
| • Freezer Temperature (-20°C) | : | Not Applicable |
| Transport Temperature | : | Transport specimen at 15°C ~25 °C (room temperature)
|
| Rejection Criteria | : |
|
| Running Day | : | Monday, Wednesday and Friday |
| Cut Off Time | : | A day before running day, 7:00 AM
|
| TAT/Releasing of Results | : | 10 days (excluding Saturdays, Sundays and Holidays)
|
| Reference Interval/Result Interpretation | : | The clinical interpretation of any staining, or absence of staining, must be complemented by histological studies and evaluation of proper controls. Evaluation must be made by a qualified pathologist within the context of the patient’s clinical history and other diagnostic tests.
|
| Limitations/Interferences | : | None Specified
|
| Frequently Asked Questions (FAQs) | : | Q. What is Immunohistochemistry? A.Immunohistochemistry or IHC refers to the process of detecting antigens (e.g., proteins) in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues. Q. Why do we need to undergo Immunohistochemistry test? A. We need to undergo Immunohistochemistry test due to following reasons:
|
| Related Words/Test | : | Monoclonal mouse anti-human cytokeratin |
| Test | : | IHC-Immunoglobulin Kappa |
| Other Test Request Name | : | • IHC-Immunoglobulin Kappa Light Chain |
| Test Composition | : | Not applicable |
| Intended Use | : | The presence of immunoglobulin light chains (kappa or lambda) on the cell surface is characteristic of clonal proliferation most often seen in multiple myeloma or lymphoproliferative disease. |
| Methodology | : | Immuno Histochemistry |
| Laboratory Section | : | Special Test |
| Special Instructions/Patient Preparations | : | No patient preparation necessary. |
| Collection/Sample Container | : | Slide holders or mailers for microscope slides; FFPE tissue blocks can either be wrapped in paper or placed in a box. |
| Specimen and Volume Requirement Note:Follow tube manufacturer recommendation. | : | • Formalin-fixed paraffin-embedded (FFPE) tissue block |
| Alternative Specimen and Volume Requirement | : | Not Applicable |
| Specimen Stability | ||
| • Room Temperature (15-25°C) | : | Indefinite |
| • Refrigerated Temperature (2-8°C) | : | Not applicable |
| • Freezer Temperature (-20°C) | : | Not applicable |
| Transport Temperature | : | • Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage. |
| Rejection Criteria | : | • Incomplete information in the Request Form |
| Running Day | : | Tuesdays, Thursdays and Fridays |
| Cut Off Time | : | Day before running day |
| TAT/Releasing of Results | : | 10 days (excluding Saturdays, Sundays and Holidays) |
| Reference Interval/Result Interpretation | : | Not Applicable |
| Limitations/Interferences | : | None specified. |
| Frequently Asked Questions (FAQs) | : | Not Applicable |
| Related Words/Test | : | Not Applicable |
| Test | : | IHC-Immunohistochem (Actin) |
| Other Test Request Name | : | IHC- Actin |
| Test Composition | : | Not applicable |
| Intended Use | : | One type of Immunohistochemical tests. IHC tests are used to diagnose a cancer as benign or malignant, to determine the stage and grade of a tumor, and/or to identify the cell type and origin of a metastasis. |
| Methodology | : | Immuno Histochemistry |
| Laboratory Section | : | Special Test |
| Special Instructions/Patient Preparations | : | No patient preparation necessary. |
| Collection/Sample Container | : | Slide holders or mailers for microscope slides; FFPE tissue blocks can either be wrapped in paper or placed in a box. |
| Specimen and Volume Requirement Note:Follow tube manufacturer recommendation. | : | • Formalin-fixed paraffin-embedded (FFPE) tissue block |
| Alternative Specimen and Volume Requirement | : | Not Applicable |
| Specimen Stability | ||
| • Room Temperature (15-25°C) | : | Indefinite |
| • Refrigerated Temperature (2-8°C) | : | Not applicable |
| • Freezer Temperature (-20°C) | : | Not applicable |
| Transport Temperature | : | • Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage. |
| Rejection Criteria | : | • Incomplete information in the Request Form |
| Running Day | : | Monday, Wednesday and Friday |
| Cut Off Time | : | Day before running day |
| TAT/Releasing of Results | : | 10 days (excluding Saturdays, Sundays and Holidays) |
| Reference Interval/Result Interpretation | : | Not Applicable |
| Limitations/Interferences | : | None specified. |
| Frequently Asked Questions (FAQs) | : | Not applicable |
| Related Words/Test | : | SMA, Anti-Smooth Muscle Antibody, ASMA, Actin Antibody, F-Actin Antibody, ACTA, Smooth Muscle Antibody |
| Test | : | IHC-Ki-67 |
| Other Test Request Name | : | - Ki-67 -MKI67 |
| Test Composition | : | Not applicable |
| Intended Use | : | - Prognostic marker for many tumors (bladder, brain, breast, kidney, lung, ovary, prostate, or thyroid malignancy): the rate of cell proliferation as assessed by Ki-67 immunoreactivity correlates with tumor grade and clinical course. - In breast cancer, the proliferative index has both prognostic and predictive value. - In malignant lymphoma, Ki-67 may be used to identify blast transformation. - In glial tumours, Ki-67 may be an aid in the differentiation between benign and malignant lesions. |
| Methodology | : | Immunohistochemistry (IHC) |
| Laboratory Section | : | Histopathology |
| Special Instructions/Patient Preparations | : | All specimens must be LABELED properly and legibly. The label must match the information on the test request form and on the provided histopathology report. |
| Collection/Sample Container | : | - Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage. - FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping. |
| Specimen and Volume Requirement Note:Follow tube manufacturer recommendation. | : | - Formalin-fixed paraffin-embedded (FFPE) tissue block - H&E stained slide, representative of the submitted tissue block. - Histopathology report for the submitted tissue block and slide - Completely filled out HPD Histopathology Request Form (HPD_F_258) Note:Submit to HPD Main Laboratory all tissue blocks and slides received. |
| Alternative Specimen and Volume Requirement | : | Not applicable |
| Specimen Stability | - FFPE tissue blocks and H&E slides are stable indefinitely when stored properly at room temperature (15oC~30oC). - Hi-Precision Diagnostics will store FFPE tissue blocks and H&E slides for 10 years, after which samples will be disposed properly according to biosafety disposal guidelines. | |
| • Room Temperature (15-25°C) | : | Indefinite |
| • Refrigerated Temperature (2-8°C) | : | Not applicable |
| • Freezer Temperature (-20°C) | : | Not applicable |
| Transport Temperature | : | Transport specimen at 15°C to 30°C |
| Rejection Criteria | : | - Incorrect/Incomplete information in the HPD Histopathology Request Form - Improper or insufficient labeling of tissue blocks or slides - Inadequate tissue available on the tissue block - Tissue blocks and slides do not correspond to its histopathology report - Specimen stored and transported outside the required temperature |
| Running Day | : | Monday to Saturday |
| Cut Off Time | : | 12:00 PM |
| TAT/Releasing of Results | : | - With Reading - 3 to 5 days after receipt of specimen/ messengers arrival or 10 working days (if with special test) - For Processing Only - 1 day after receipt of specimen/ messengers arrival Note: Additional 2 days may be necessary if specimen needs further evaluation and confirmation by another Pathologist |
| Reference Interval/Result Interpretation | : | - The clinical interpretation of any staining, or absence of staining, must be complemented by histological studies and evaluation of proper controls. - Evaluation must be made by a qualified pathologist within the context of the patient’s clinical history and other diagnostic tests. |
| Limitations/Interferences | : | - Ventana recommends fixation in 10% NBF for 6-48 hours. - Fixation for less than 6 hours is not recommended. |
| Frequently Asked Questions (FAQs) | : | Q. What is Immunohistochemistry? A.Immunohistochemistry or IHC refers to the process of detecting antigens (e.g., proteins) in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues. Q. Why do we need to undergo Immunohistochemistry test? A. - Confirm a pathologic diagnosis - Differentiate between benign or malignant tumor - Detect among different types of tumor - Monitor prognosis response to treatment Q. What is ki67 proliferation marker? A.The expression ofKi67is strongly associated with tumor cellproliferationand growth, and is widely used in routine pathological investigation as aproliferation marker. The nuclear proteinKi67(pKi67) is an established prognostic and predictive indicator for the assessment of biopsies from patients with cancer. |
| Related Words/Test | : | - Immunohistochemistry (IHC) - Ki-67 -MKI67 |
| Test | : | IHC-Lynch Syndrome Panel |
| Other Test Request Name | : |
|
| Test Composition | : |
|
| Intended Use | : | Genetic defects in these four mismatch repair proteins underlie most cases of Lynch syndrome or hereditary non-polyposis colorectal carcinoma (HNPCC). enetic defects in these four mismatch repair proteins underlie most cases of Lynch syndrome or hereditary non-polyposis colorectal carcinoma (HNPCC). This IHC panel looks for loss of expression of one or more of these proteins in cases of suspected HNPCC in tumor tissues from colorectal carcinomas, endometrial carcinomas, sebaceous adenoma/carcinoma or other adnexal tumors. If IHC results are abnormal, guidance is provided in the report on the recommended next step in the workup. |
| Methodology | : | Immunohistochemistry (IHC) |
| Laboratory Section | : | Special Test |
| Special Instructions/Patient Preparations | : | No patient preparation necessary. |
| Collection/Sample Container | : | Slide holders or mailers for microscope slides; Formalin-fixed, paraffin-embedded tissue collected in a paraffin block bag |
| Specimen and Volume Requirement Note:Follow tube manufacturer recommendation. | : |
|
| Alternative Specimen and Volume Requirement | : | Not Applicable |
| Specimen Stability | ||
| • Room Temperature (15-25°C) | : | Indefinite |
| • Refrigerated Temperature (2-8°C) | : | Not Applicable |
| • Freezer Temperature (-20°C) | : | Not Applicable |
| Transport Temperature | : |
|
| Rejection Criteria | : |
|
| Running Day | : | Batch Running |
| Cut Off Time | : | Monday, 12:00 PM |
| TAT/Releasing of Results | : | 2 weeks after cut-off (excluding Saturdays, Sundays and Holidays) |
| Reference Interval/Result Interpretation | : | Not Applicable |
| Limitations/Interferences | : | None Specified. |
| Frequently Asked Questions (FAQs) | : | Not Applicable |
| Related Words/Test | : | Not Applicable. |