Laboratory Test Preparation

Test:Trichome Staining
Other Test Request Name:

Not applicable

Test Composition:

Not Applicable

Intended Use:

Not Applicable

Methodology:

Special Staining

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:

No Patient Preparation

Collection/Sample Container:

Slide holders or mailers for microscope slides; FFPE tissue blocks can either be wrapped in paper or placed in a box.

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

• Formalin-fixed paraffin-embedded (FFPE) tissue block
• 1 H&E stained slide representative of the submitted tissue block.
• Histopathology report for the submitted tissue block

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

Indefinite

•   Refrigerated Temperature (2-8°C):

Not Applicable

•   Freezer Temperature (-20°C):

Not Applicable

Transport Temperature:

• Microscope slides should be placed in slide holders or mailers and should be adequately padded before placing in a shipping/packaging container to prevent breakage.
• FFPE tissue blocks must be placed in a box and avoid direct sunlight to prevent melting and disorientation during transport or shipping.
• Transport specimen at 15°C ~25 °C (room temperature)

Rejection Criteria:

• Incomplete information in the Request Form
• Improper or insufficient labeling of tissue blocks or slides
• Inadequate tissue available on the tissue block

Running Day:

Monday to Friday

Cut Off Time:

2 PM

TAT/Releasing of Results:

10 days (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Not Applicable

Limitations/Interferences:

Not Applicable

Frequently Asked Questions (FAQs):

Not Applicable

Related Words/Test:

Not Applicable

 

Test:Triglycerides (ENZYMATIC)
Other Test Request Name:

• TG
• TRIG
• Trigly

Test Composition:

Not applicable

Intended Use:

The determination of triglycerides is utilized in the diagnosis and treatment of patients having diabetes mellitus, nephrosis, liver obstruction, lipid metabolism disorders and numerous other endocrine diseases.

Methodology:

Colorimetric, Enzymatic Method

Laboratory Section:

Chemistry

Special Instructions/Patient Preparations:

10-14 hours fasting.
Note:
• Prolonged fasting is highly discouraged since various metabolic changes associated with starvation may begin to develop. Water intake is acceptable.
• Note in clinical info if patient drank more than 4 glasses of water during fasting period, ask patient for medical history as this is rather unusual.
• Note in clinical info in case patient overfasts and insists on proceeding test.

Collection/Sample Container:

Red or Gold tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

1-3 mL Serum

Alternative Specimen and Volume Requirement:

1-3mL Plasma (Li-heparin and K2-EDTA)

Specimen Stability  
•   Room Temperature (15-25°C):

Not Specified

•   Refrigerated Temperature (2-8°C):

10 Days

•   Freezer Temperature (-20°C):

3 Months

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:
  • Specimens that failed serum index criteriaExceeded sample stability requirement
  • Quantity not sufficient
  • Improperly labeled specimen
  • Improper collection tube used
Running Day:

Daily

Cut Off Time:

Monday to Saturday: 10:00 PM

Sunday: 6:00PM

TAT/Releasing of Results:

RUNNING TIME: 6:00 AM onwards

RELEASING TIME: ROUTINE
• 4 hours after receipt of specimen/ arrival of messenger
RELEASING TIME: STAT
• After 2½ hours from extraction/messenger arrival

Reference Interval/Result Interpretation:

NCEP Recommendations:
Borderline High: 1.69-2.25 mmol/L(149.57-199.12 mg/dL)
High: 2.26-5.64 mmol/L (200.01-499.14 mg/dL)
Very High: >/= 5.65 mmol/L (>/= 500.02 mg/dL)

Limitations/Interferences:

• Endogenous unesterified glycerol in the sample will falsely elevate serum triglycerides.
• Dicynone (Etamsylate) at therapeutic concentrations may lead to false-low results.
• Ascorbic acid and calcium dobesilate cause artificially low triglyceride results. Intralipid is directly measured as analyte in this assay and leads to high triglyceride results.
• Acetaminophen intoxications are frequently treated with N-Acetylcysteine. N-Acetylcysteine at a plasma concentration above 166 mg/L and the Acetaminophen metabolite N-acetyl-p-benzoquinone imine (NAPQI) independently may cause falsely low results.
• Venipuncture should be performed prior to the administration of Metamizole. Venipuncture immediately after or during the administration of Metamizole may lead to falsely low results. A significant interference may occur at plasma Metamizole concentrations above 0.05 mg/mL.
• In very rare cases, gammopathy, in particular type IgM (Waldenstrom’s
macroglobulinemia), may cause unreliable results.

Frequently Asked Questions (FAQs):

Q: When should testing for cholesterol/lipid profile be done?
A: In all adults aged 20 years or older, a fasting Lipid Profile should be obtained once every 5 years.

Q: Can cholesterol be done on non-fasting samples?
A: If the testing opportunity is nonfasting, only the values for total cholesterol and HDL cholesterol will be usable.

Related Words/Test:

Cholesterol, HDL Cholesterol, LDL Cholesterol, Direct LDL Cholesterol, VLDL Cholesterol, Lipid Profile, Cardiac Risk Assessment

 

Test:Triidothyronine Uptake-Temporarily Unavailable (currently not accepting samples)
Other Test Request Name:
  • T3 Resin Uptake
  • T3 Uptake
Test Composition:

Not applicable

Intended Use:

T3 uptake (T3U) is used with measurement of thyroxine (T4) to calculate the free T4 index. The calculated free T4 is useful in the assessment of thyroid diseases. Elevations are associated with hyperthyroidism or thyroid hormone resistance whereas low concentrations are associated with hypothyroidism.

Methodology:

Immunoassay (IA)

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:

No patient preparation necessary.

Collection/Sample Container:

Red or Gold Tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

3 mL Serum

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

24 Hours

•   Refrigerated Temperature (2-8°C):

3 Days

•   Freezer Temperature (-20°C):

3 Days

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:
  • Hemolyzed specimen
  • Markedly lipemic specimen
  • Exceeded sample stability requirement
  • Quantity not sufficient
  • Improperly labeled specimen
  • Improper collection tube used
Running Day:

Monday to Friday

Cut Off Time:

Monday to Friday, 7:00 AM

TAT/Releasing of Results:

3 days after running day (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Available upon request

Limitations/Interferences:

None Specified.

Frequently Asked Questions (FAQs):

Not Applicable

Related Words/Test:

Thyroxine-binding globulin (TBG), T3, thyroid hormone

 

Test:Trisomy 21 (FISH) - (BY APPOINTMENT PER PATIENT)
Other Test Request Name:

Not applicable

Test Composition:

Not applicable

Intended Use:

A common chromosome disorder, often called Down's syndrome, due to an extra chromosome number 21 (trisomy 21). The chromosome abnormality affects both the physical and intellectual development of the individual.

Methodology:

FISH

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:
  • No patient preparation necessary.
  • Call Special Test Section (By Appointment/Schedule.

NOTE: Do not collect specimen without approval of Special Test Section

Collection/Sample Container:

Sodium Heparin

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

4 mL Sodium Heparinized whole blood (Peripheral Blood)

Alternative Specimen and Volume Requirement:

Bone Marrow: 2 pcs of 4 mL Sodium Heparinized whole blood

Specimen Stability  
•   Room Temperature (15-25°C):

24 Hours

•   Refrigerated Temperature (2-8°C):

Not applicable

•   Freezer Temperature (-20°C):

Not applicable

Transport Temperature:

Transport specimen at 15 – 25 °C

Rejection Criteria:
  • Hemolyzed specimen
  • Markedly lipemic specimen
  • Exceeded sample stability requirement
  • Quantity not sufficient
  • Improperly labeled specimen
  • Improper collection tube used
  • Clotted Sample
  • Over-filled or Under-filled tube
Running Day:

Monday-Friday 

Cut Off Time:

Monday-Friday; 700 AM

TAT/Releasing of Results:

10 days after running (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Not Applicable

Limitations/Interferences:

None specified.

Frequently Asked Questions (FAQs):

Not Applicable

Related Words/Test:

Down syndrome, chromosal disorder, chromosomal abnormality

 

Test:Troponin I (Qualitative) -- Temporary Unavailable
Other Test Request Name:

• Cardiac Troponin I (CTNI) (Qualitative test)
• TnI (Qualitative test)

• Cardiac-specific Troponin I (Qualitative test)

Test Composition:

Not Applicable

Intended Use:

• For Qualitative Determination of Cardiac Troponin I (CTNI) in human serum and plasma.
• Used as an aid in the determination of myocardial damage in patients with acute myocardial infraction (AMI). it can be detected in blood at 4-6 hours following the onset of symptoms, reaches a peak concentration at 12-24 hours, and remains elevated for 5-7 days

Methodology:

Rapid Immunochromatography

Laboratory Section:

Serology

Special Instructions/Patient Preparations:

No patient preparation necessary.

Collection/Sample Container:

Red or Gold tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

1-2mL Serum

Alternative Specimen and Volume Requirement:

1-2mL Plasma (EDTA, Heparin, Na Citrate)

Specimen Stability  
•   Room Temperature (15-25°C):

Not Specified

•   Refrigerated Temperature (2-8°C):

24 Hours

•   Freezer Temperature (-20°C):

>24 Hours

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:

• Improperly labeled specimens
• Hemolyzed specimens
• Quantity not sufficient
• Specimen stored exceeded outside required stability

Running Day:

Daily

Cut Off Time:

Monday to Saturday
• 8:00 PM

Sunday
• 3:00 PM

TAT/Releasing of Results:

ROUTINE
• 3 hours after receipt of specimen/ arrival of messenger
STAT
• 1½ hours from extraction/messenger arrival

Reference Interval/Result Interpretation:

NEGATIVE

Limitations/Interferences:

A positive result may only be used as an indicator of myocardial damage; diagnosis of myocardial damage requires further confirmation.

Frequently Asked Questions (FAQs):

Not Applicable

Related Words/Test:

Cardiac Damage, Heart Attack, TnT, cTnT, Cardiac-specific Troponin T, Myocardial Infarction, MI

 

Test:Troponin T (Qualitative)
Other Test Request Name:

• Cardiac Troponin T (cTnT) Qualitative

• Cardiac-specific Troponin T Qualitative

Test Composition:

Not Applicable

Intended Use:

For qualitative determination of cardiac Troponin T (cTnT) in anticoagulated venous or arterial blood as an aid in the diagnosis of patients with suspected myocardial cell damage. It is usually requested 2 to more than 10 hours following the onset of symptoms.

Methodology:

Sandwich Complex

Laboratory Section:

Serology

Special Instructions/Patient Preparations:

No patient preparation necessary.

Collection/Sample Container:

EDTA (purple top)

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

2mL EDTA Whole blood

Alternative Specimen and Volume Requirement:

2mL Whole blood (Heparin)

Specimen Stability  
•   Room Temperature (15-25°C):

8 Hours

•   Refrigerated Temperature (2-8°C):

Unacceptable

•   Freezer Temperature (-20°C):

Unacceptable

Transport Temperature:

Transport specimen at 15-25°C (room temperature)

Rejection Criteria:

• Specimen stored exceeded stability requirement
• Improper use of anticoagulant
• Insufficient volume of blood to anticoagulant
• Improperly labeled specimens
• Quantity not sufficient

Running Day:

Daily

Cut Off Time:

Monday to Saturday
• 8:00 PM

Sunday
• 3:00 PM

TAT/Releasing of Results:

ROUTINE
• 3 hours after receipt of specimen/ arrival of messenger
STAT
• 1½ hours from extraction/messenger arrival

Reference Interval/Result Interpretation:

NEGATIVE

Limitations/Interferences:

High doses of biotin ( i.e. >5mg/day) may affect results. Samples should not be taken until at least 8 hours following the last biotin administration. High concentrations of lipoic acid may yield lower measurement values.

Frequently Asked Questions (FAQs):

Not Applicable

Related Words/Test:

Cardiac Damage; Heart Attack, TnI; cTnI; Cardiac-specific Troponin I

 

Test:Troponin T (Quantitative)
Other Test Request Name:

• Cardiac Troponin T (cTnT) Quantitative

• Cardiac-specific Troponin T Quantitative

Test Composition:

Not Applicable

Intended Use:

For Quantitative determination of cardiac Troponin T (cTnT) in anticoagulated venous or arterial blood as an aid in the diagnosis of patients with suspected myocardial cell damage. It is usually requested 2 to more than 10 hours following the onset of symptoms.

Methodology:

Sandwich Complex

Laboratory Section:

Serology

Special Instructions/Patient Preparations:

No patient preparation necessary.

Collection/Sample Container:

Heparin (Green Top)

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

2mL Heparin Whole Blood

Alternative Specimen and Volume Requirement:

Not applicable

Specimen Stability  
•   Room Temperature (15-25°C):

8 Hours

•   Refrigerated Temperature (2-8°C):

Unacceptable

•   Freezer Temperature (-20°C):

Unacceptable

Transport Temperature:

Transport specimen at 15-25°C (room temperature)

Rejection Criteria:

• Specimen stored exceeded stability requirement
• Improper use of anticoagulant
• Insufficient volume of blood to anticoagulant
• Improperly labeled specimens
• Quantity not sufficient

Running Day:

Daily

Cut Off Time:

Monday to Saturday
• 8:00 PM

Sunday
• 3:00 PM

TAT/Releasing of Results:

ROUTINE
• 3 hours after receipt of specimen/ arrival of messenger
STAT
• 1½ hours from extraction/messenger arrival

Reference Interval/Result Interpretation:

<50.00 ng/L: Acute MI not likely, but possible
50.00~100 ng/L: Acute MI possible
100.00~2,000.00 ng/L: Acute MI likely
>2,000.00 ng/L: Acute MI very likely

Limitations/Interferences:

• High doses of biotin ( i.e. >5mg/day) may affect results. Samples should not be taken until at least 8 hours following the last biotin administration.
• High concentrations of lipoic acid may yield lower measurement values.
• Skeletal muscle troponin T up to 500,000 ng/L leads to a maximum increase of 10%; at 1,000,000 ng/L, the increase may be up to 30%.
• Heterophilic antibodies may react to give falsely elevated or decreased results.

Frequently Asked Questions (FAQs):

Not Applicable

Related Words/Test:

Cardiac Damage; Heart Attack, TnI; cTnI; Cardiac-specific Troponin I

 

Test:Trypanosoma Cruzi
Other Test Request Name:
  • Trypanosoma Cruzi
  • South American trypanosomiasis
  • Chagas disease - serology
  • Trypanosoma
  • Anti-Trypanosoma cruzi antibodies
  • Trypanosoma cruzi Antibody, IgG

 

Test Composition:

Not applicable

Intended Use:

Antibodies are found in most patients with acute or chronic American Trypanosomiasis.

Methodology:

Immunoassay (IA)

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:

No patient preparation necessary.

Collection/Sample Container:

Red or Gold Tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

3 mL Serum

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

3 Days

•   Refrigerated Temperature (2-8°C):

7 Days

•   Freezer Temperature (-20°C):

28 days

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:

• Hemolyzed specimen
• Markedly lipemic specimen
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used

Running Day:

Batch Running

Cut Off Time:

Monday, 12:00 PM

 

TAT/Releasing of Results:

2 Weeks after cut-off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Available upon request

Limitations/Interferences:

None specified.

Frequently Asked Questions (FAQs):

Not Applicable

Related Words/Test:

Parasitic euglenoid protozoan

 

Test:Tryptase
Other Test Request Name:

Not applicable

Test Composition:

Not applicable

Intended Use:

Tryptase is secreted almost exclusively by mast cells and is only found in the blood after these cells have been stimulated by some agonist, e.g. a drug or hymenopteran venom. Its levels correlate with the severity of the anaphylaxis (e.g. the case of curare). High levels are also found in systemic mastocytosis.

Methodology:

Fluorescent enzyme-immunoassay

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:

No patient preparation necessary.

Collection/Sample Container:

Red or Gold Tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

3 mL Serum

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

24 Hours

•   Refrigerated Temperature (2-8°C):

7 Days

•   Freezer Temperature (-20°C):

28 Days

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:
  • Hemolyzed specimen
  • Markedly lipemic specimen
  • Exceeded sample stability requirement
  • Quantity not sufficient
  • Improperly labeled specimen
  • Improper collection tube used
Running Day:

Batch Running

Cut Off Time:

Friday, 4:00 PM

TAT/Releasing of Results:

3 weeks after cut-off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Available upon request

Limitations/Interferences:

None specified.

Frequently Asked Questions (FAQs):

Not Applicable

Related Words/Test:

Mast Cell Tryptase, Alpha Tryptase, Beta Tryptase, Mature Tryptase, Total Tryptase

 

Test:TSH (ECLIA)
Other Test Request Name:

• Thyroid Simulating Hormone
• Thyrotropin

Test Composition:

Not applicable

Intended Use:

This serves as an initial test in thyroid diagnostics.

Methodology:

Electrochemiluminescence Immunoassay (ECLIA)

Laboratory Section:

Immunology

Special Instructions/Patient Preparations:

Samples should not be taken from patients receiving therapy with high biotin doses (i.e. > 5 mg/day) until at least 8 hours following the last biotin administration.

Collection/Sample Container:

Red or Gold tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

1-3 mL Serum

Alternative Specimen and Volume Requirement:

1-3 mL Plasma (Li-, Na-, NH4 -heparin, K3-EDTA, Sodium Citrate, Sodium Fluoride, Potassium Oxalate)

Specimen Stability  
•   Room Temperature (15-25°C):

Not specified

•   Refrigerated Temperature (2-8°C):

7 Days

•   Freezer Temperature (-20°C):

1 Month (Freeze only once)

Transport Temperature:

Transport specimen at 2°C – 8 °C (with cold packs)

Rejection Criteria:

• Hemolyzed specimen
• Markedly lipemic sample
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used

Running Day:

Daily

Cut Off Time:

Monday to Saturday:10:00 PM

Sunday:6:00PM

TAT/Releasing of Results:

ROUTINE (on running days)
• 4 hours after receipt of specimen/ arrival of messenger
STAT (on running days only)
• 2½ hours from extraction/messenger arrival

Reference Interval/Result Interpretation:

0.27-4.20 uIU/mL

Limitations/Interferences:

• Samples should not be taken from patients receiving therapy with high biotin doses (i.e. > 5 mg/day) until at least 8 hours following the last biotin administration.
• In rare cases, interference due to extremely high titers of antibodies to analyte-specific antibodies, streptavidin or ruthenium can occur.
• The presence of autoantibodies may induce high molecular weight complexes (macro-TSH) which may cause unexpected high values of TSH.

Frequently Asked Questions (FAQs):

Not applicable

Related Words/Test:

Thyroid Function Test, Free T4, Free T3 and Total T3, Thyroid Panel, Thyroid Antibodies

 

Test:TSH (IRMA) Temporarily Unavailable (currently not accepting samples)
Other Test Request Name:
  • Thyroid Stimulating Hormone- Immunoradiometric Assay
  • 125-I hTSH IRMA
Test Composition:

Not Applicable

Intended Use:

Aids in the diagnosis of thyroid disorders.

Used in the evaluation of the functional integrity of the hypothalamic-pituitary axis

Methodology:

Immunoradiometric Assay (IRMA)

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:

No patient preparation necessary.

Collection/Sample Container:

Gold or Red Top Tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

5 mL Serum

Alternative Specimen and Volume Requirement:

Not Applicable.

Specimen Stability  
•   Room Temperature (15-25°C):

Not Applicable

•   Refrigerated Temperature (2-8°C):

24 Hours

•   Freezer Temperature (-20°C):

30 Days

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:
  • Insufficient volume
  • Improperly labeled specimens
  • Markedly lipemic sample
  • Hemolyzed sample
  • Repeated freezing and thawing
  • Exceeded sample stability requirement
Running Day:

Friday

Cut Off Time:

Thursday, 7:00 AM

TAT/Releasing of Results:

3 days after cut off (excluding saturday, sunday and holidays)

Reference Interval/Result Interpretation:

Not Applicable.

Limitations/Interferences:

None Specified.

Frequently Asked Questions (FAQs):

Not Applicable 

Related Words/Test:

Not Applicable.

 

Test:TSH Receptor Antibody (ECLIA)
Other Test Request Name:
 
  • Anti TSHR
  • Anti TSH Receptor
  • Thyroid Stimulating Hormone Receptor Antibody
  • TSHR Antibody
  • TRAb
  • TSH Receptor Binding Antibody
  • Thyrotropin Binding Immunoglobulin
  • TBII
  • Thyrotropin Receptor Antibody

 

Test Composition:

Not Applicable

Intended Use:

It is used to aid in the differential diagnosis of Grave's disease.

Methodology:

ELECTROCHEMILUMINISCENCE IMMUNOASSAY (ECLIA)

Laboratory Section:

Immunology

Special Instructions/Patient Preparations:
  • No patient preparation required
  • Do not use under samples from patients under Sodium Heparin therapy
  • Samples should not be taken from patients receiving therapy with high biotin doses (i.e. > 5 mg / day) until atleast  8 hours following  the last biotin  administration.
Collection/Sample Container:

Red or Gold tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

1-3 mL of Serum

Alternative Specimen and Volume Requirement:

Not applicable

Specimen Stability  
•   Room Temperature (15-25°C):

not specified

•   Refrigerated Temperature (2-8°C):

3 Days

•   Freezer Temperature (-20°C):

1 Month (Freeze only once)

Transport Temperature:

Transport sample at 2°C – 10 °C (with cold packs)

Rejection Criteria:
  • Exceeded sample stability requirement
  • Quntity not sufficient
  • Improperly labeled sample
  • Improper collection tube used
  • Sample stored/transported outside the temperature range
  • Heat inactivated specimens
  • Pooled specimens
  • Samples stabilized with azide
Running Day:

Tuesday, Thursday and Saturday

Running Time: 12:00 PM- 7: 00 PM

Cut Off Time:

6:00 PM

TAT/Releasing of Results:

Running Time: 12:00 PM – 7:00 PM

TAT: 4:00 PM onwards on running day
Reference Interval/Result Interpretation:

Cut off value:  1.75 IU/L

Limitations/Interferences:
  • Do not use samples from patients under sodium heparin therapy.
  • In rare cases, interference due to extremely high titers of antibodies to analyte-specific antibodies, streptavidin or ruthenium can occur.
 
Frequently Asked Questions (FAQs):

Q: What is this test for?

A: TSH Receptor Antibody is the recommended first-line test for detection of thyrotropin receptor (TSHR) antibodies, may be used in the following situations:

  • Differential diagnosis of etiology of thyrotoxicosis in patients with ambiguous clinical findings and/or contraindicated (eg, pregnant or breast-feeding) or nondiagnostic thyroid radioisotope scans
  • Diagnosis of clinically suspected Graves disease (eg, extrathyroidal manifestation of Graves disease include endocrine exophthalmos, pretibial myxedema, thyroid acropachy) in patients with normal thyroid function tests
  • Determining the risk of neonatal thyrotoxicosis in a fetus of a pregnant female with active or past active Graves disease
  • Differential diagnosis of gestational thyrotoxicosis versus first trimester manifestation or recurrence of Graves disease
  • Assessing the risk of Graves disease relapse after antithyroid drug treatment
Related Words/Test:

Thyroid antibodies, Anti Thyroglobulin, Anti TPO, TSH, T3, T4, FT3, FT4, Thyroid auto antibodies

 

 

Test:Typhoid Screening IgG/IgM
Other Test Request Name:
  • Salmonella typhi screening test
  • Salmonella typhi IgG /IgM
  • Previously known asTyphidot Test
Test Composition:

Not applicable

Intended Use:

For screening test of Typhoid fever.  It can detect and differentiate IgG and IgM antibodies to Salmonella typhi. 

Methodology:

Solid Phase Immunochromatography

Laboratory Section:

Serology

Special Instructions/Patient Preparations:

No patient preparation required.

Collection/Sample Container:

Plain Red Top or Gold Top

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

1-2 mL Serum

Alternative Specimen and Volume Requirement:

1-2 mL Plasma (EDTA, Heparin, Citrate)

Specimen Stability  
•   Room Temperature (15-25°C):

Not specified

•   Refrigerated Temperature (2-8°C):

7 days 

•   Freezer Temperature (-20°C):

>2 weeks

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:

•        Contaminated and markedly lipemic serum

•        Improperly labeled specimens

•        Hemolyzed specimens

•        Quantity not sufficient

•        Specimen stored exceeded outside required stability

Running Day:

Daily

Cut Off Time:

Monday up to Saturday
8:00 PM
Sunday
3:00 PM

TAT/Releasing of Results:

ROUTINE
3 hours after receipt of specimen/ arrival of messenger

STAT
1½ hours from extraction/messenger arrival

Reference Interval/Result Interpretation:

Salmonella typhi IgG:  NEGATIVE

Salmonella typhi IgM: NEGATIVE

Limitations/Interferences:

None identified.

Frequently Asked Questions (FAQs):

Not Applicable

Related Words/Test:

Typhoid Fever, Salmonella typhi

 

Test:Tzanck Smear
Other Test Request Name:Multinucleated Giant Cell
Test Composition:Not applicable
Intended Use:It is a microscopic test that helps diagnose viral infections in which blistering vesicles are presnt such as herpes simplex and varicella zoster.
Methodology:Direct Microscopic Examination
Laboratory Section:Clinical Microscopy
Special Instructions/Patient Preparations:No patient preparation necessary.

NOTE: Do not wrap the slide with paper.
Collection/Sample Container:• Slides should be placed in slide holders or mailers before placing in a packaging container
• In cases wherein slide mailers/ holders are not available, place slides in a hard cardboard box/container to prevent breakage and releasing the pinworms to the environment.
Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:2 smears prepared from the scrapings from the base of lesion/lesion are recommended. (Direct smear of material collected from the skin lesions, usually a vesicle smeared on a glass slides and fixed in 95% alcohol).
Alternative Specimen and Volume Requirement:Not applicable
Specimen Stability  
•   Room Temperature (15-25°C):• Unfixed slides must be sent to laboratory immediately . • Slides fixed in 95% alcohol are stable for 1-3 days
•   Refrigerated Temperature (2-8°C):Not applicable
•   Freezer Temperature (-20°C):Not applicable
Transport Temperature:Transport specimens immediately to HPD Laboratory at room temperature (15-25°C)
Rejection Criteria:Mislabeled or unlabeled specimens
Running Day:Daily
Cut Off Time:Monday to Saturday
• 6:00 PM

Sunday
• 3:00 PM
TAT/Releasing of Results:2 Days
Reference Interval/Result Interpretation:NEGATIVE – No multinucleated giants cells are seen
Limitations/Interferences:Wrapping the wet smears with paper will cause it to adhere resulting to artificial cellular distortion.
Frequently Asked Questions (FAQs):Not applicable
Related Words/Test:• Herpes simplex
• Varicella zoster

 

Test:Uncle Test (DNA Testing)
Other Test Request Name:

Not Applicable

Test Composition:

Not Applicable

Intended Use:

The DNA Uncle test determines the liklihood that an alleged uncle is the true biological uncle of a child.

 

Methodology:

DNA Analysis

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:
  • Hard copy RTPCR negative results for BOTH PARTIES are needed 2-3 days from the date of collection.
  • Inform Special Test Section before the scheduled sample collection for the collection kit.
  • Request DNA Collection Kit to Special test section 3 days prior of swab collection.
  • Only trained doctors/RMTs are allowed to collect samples.

Sample collection/handling:

  1. Each collection kit (provided by HP Main Lab) includes 4 swabs.
  2. Collect sample using the 4 swabs for each patient (i.e. 4 swabs for uncle, 4 swabs for child)
  3. Return swabs to its sterile package and place it inside the provided envelope.
  4. Encircle whether sample came from uncle or child.                  NOTE: Sample from uncle and child must be placed in separate envelopes.
  5. Completely fill up the required information in the envelope.       NOTE: Signatures of patient and collector is required. For minors, legal guardian must sign on the envelope containing the child's sample.
  6. Remove adhesive cover and seal the envelope with sample.
  7. Send sample immediately to HP Main Lab.

Document Requirements:

  • Birth Certificate/School I.D of child, actual and  2 photocopies
  • Valid I.D of uncle, actual and  2 photocopies
  • Completely filled up request form
  • Completely filled up disclaimer form
  • Mother's consent; Valid ID of mother, actual and 2 photocopies
  • Valid ID of requesting party (if not the alleged uncle, actual and 2 photocopies

NOTE: Double check completeness and correctness of information before sending to HP Main Lab to avoid delay ing processing of sample.

Collection/Sample Container:

DNA Collection kit(provided by Special Test)

 

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

4 swabs each from uncle and child

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

7 Days

•   Refrigerated Temperature (2-8°C):

Not Applicable

•   Freezer Temperature (-20°C):

Not Applicable

Transport Temperature:

Transport specimen at 15°C ~25 °C (room temperature)

 

Rejection Criteria:
  • Incomplete details of patient, including signatures
  • Unlabelled sample
  • Incorrect collection container used
  • Incomplete document requirement

 

Running Day:

Batch Running

Cut Off Time:

Friday, 12:00 PM

TAT/Releasing of Results:

2 weeks after cut-off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Not Applicable

Limitations/Interferences:

None Specified.

Frequently Asked Questions (FAQs):

Not Applicable

Related Words/Test:

DNA test that establishes whether a man is the biological father of a child