Laboratory Test Preparation

Test:Upper Abdomen Ultrasound
Other Test Request Name:

-

Test Composition:Not Applicable
Intended Use:
Ultrasound imaging or sonography is considered to be a non-invasive procedure and does not involve ionizing radiation which is usual in X-rays. An ultrasound scan is not only useful in assessing the structure of organs and different internal structures but can also show the movement of organs in real time. It may be used to assess the size and location of organs and structures of the following:
  • Liver
  • Common Bile Duct
  • Gall Bladder
  • Pancreas
  • Spleen
Methodology:Not Applicable
Laboratory Section:Not Applicable
Special Instructions/Patient Preparations:
PREPARATION
  • No food intake for at least 6 hours.
  • NOTE: No need to fast if patient has undergone cholecystectomy or surgical removal of gall bladder.
  • Drinking water is not prohibited.
  • Bring previous ultrasound result for the comparison.
Collection/Sample Container:Not Applicable
Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:Not Applicable
Alternative Specimen and Volume Requirement:Not Applicable
Specimen Stability  
•   Room Temperature (15-25°C):Not Applicable
•   Refrigerated Temperature (2-8°C):Not Applicable
•   Freezer Temperature (-20°C):Not Applicable
Transport Temperature:Not Applicable
Rejection Criteria:Not Applicable
Running Day:Not Applicable
Cut Off Time:Not Applicable
TAT/Releasing of Results:

-

Reference Interval/Result Interpretation:

-

Limitations/Interferences:Not Applicable
Frequently Asked Questions (FAQs):

-

Related Words/Test:Not Applicable

 

Test:Uric Acid (ENZYMATIC)
Other Test Request Name:

• UA
• Blood Uric Acid

Test Composition:

Not applicable

Intended Use:

Uric acid measurements are used in the diagnosis and treatment of numerous renal and metabolic disorders, including renal failure, gout, leukemia, psoriasis, starvation or other wasting conditions, and of patients receiving cytotoxic drugs.

Methodology:

Enzymatic colorimetric

Laboratory Section:

Chemistry

Special Instructions/Patient Preparations:

8-12 hours fasting.
Note:
• Prolonged fasting is highly discouraged since various metabolic changes associated with starvation may begin to develop. Water intake is acceptable.
• Note in clinical info if patient drank more than 4 glasses of water during fasting period, ask patient for medical history as this is rather unusual.
• Note in clinical info in case patient overfasts and insists on proceeding test.

Collection/Sample Container:

Red or Gold tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

1-3 mL Serum

Alternative Specimen and Volume Requirement:

1-3mL Plasma (Li-heparin)

Specimen Stability  
•   Room Temperature (15-25°C):

Not Specified

•   Refrigerated Temperature (2-8°C):

5 Days

•   Freezer Temperature (-20°C):

6 Months

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:
  • Specimens that failed serum index requirement
  • Markedly lipemic specimen
  • Exceeded sample stability requirement
  • Quantity not sufficient
  • Improperly labeled specimen
  • Improper collection tube used
Running Day:

Daily

Cut Off Time:

Monday to Saturday: 6:00 PM

Sunday: 4:00PM

TAT/Releasing of Results:

ROUTINE
• 4 hours after receipt of specimen/ arrival of messenger
STAT
• After 2½ hours from extraction/messenger arrival

Reference Interval/Result Interpretation:

MALE: 202.30-416.50 umol/L (3.42-7.04 mg/dL)
FEMALE: 142.80-339.20 umol/L (2.41-5.73 mg/dL)

Limitations/Interferences:

• Calcium dobesilate causes artificially low uric acid results.
• Uricase reacts specifically with uric acid. Other purine derivatives can inhibit the uric acid reaction.
• Dicynone (Etamsylate) at therapeutic concentrations may lead to false?low results.
• Acetaminophen intoxications are frequently treated with N?Acetylcysteine. N?Acetylcysteine at the therapeutic concentration when used as an antidote and the Acetaminophen metabolite N?acetyl?p?benzoquinone imine (NAPQI) independently may cause falsely low results.
• Venipuncture should be performed prior to the administration of Metamizole. Venipuncture immediately after or during the administration of Metamizole may lead to falsely low results.
• In very rare cases, gammopathy, in particular type IgM (Waldenstrom’s macroglobulinemia), may cause unreliable results.

Frequently Asked Questions (FAQs):

Not applicable

Related Words/Test:

Gout, Synovial Fluid Analysis, Kidney Stone Analysis, Kidney Stone Risk Panel

 

Test:Urine 5HIAA
Other Test Request Name:
  • Serotonin metabolite
  • HIAA 5
  • 5-HIAA
  • Hydroxyindoleacetic - 5
Test Composition:

Not applicable

Intended Use:

Used to help diagnose and monitor carcinoid tumors.

Used to find an abnormal amount of serotonin being made in your body.

A serotonin metabolite, its excretion is increased in small intestine carcinoid tumours. It may also be elevated in small cell lung cancer, non-Beta pancreatic endocrine tumours, ovarian or gallbladder cancer and in some benign diseases (severe coronary failure, Hartnup's disease).

Methodology:

Liquid Chromatography Mass Spectrometry (LCMS/MS)

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:
  • Patient should avoid alcohol, coffee, tea, tobacco (including use of nicotine patch), bananas, citrus fruits and strenuous exercise prior for 48 Hours prior to collection.
  • Note Date and Time of Collection (Start/End)

Collection Procedure:

  1. Instruct the patient to void at the beginning of collection period and discard the specimen.
  2. Collect all urine including the final specimen voided at the end of collection period.
  3. Mix 24 hour urine collected.
  4. Label the bottle with patient name, date & time collection started, date & time collection ended and total volume measured.
  5. Note total volume.
  6. Submit the specimen to HPD
Collection/Sample Container:

Leak proof clean container without preservative

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

100 mL of 24 hour Urine

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

24 Hours

•   Refrigerated Temperature (2-8°C):

7 Days

•   Freezer Temperature (-20°C):

28 Days

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:
  • Quantity not sufficient
  • Received room temperature
  • Improperly labeled specimen
  • Improper urine collection
Running Day:

Batch Running

Cut Off Time:

Friday, 4:00 PM

TAT/Releasing of Results:

2 weeks after cut-off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Available upon request

Limitations/Interferences:

Contents from the following food and drinks may interfere with the assay:
• Alcohol or any caffeinated drinks like coffee, tea or soft drinks.
• Bananas
• Tomato
• Kiwi
• Pineapple
• Avocado
• Butternuts
• Walnuts and pecans
• Eggplant
• Plums

Frequently Asked Questions (FAQs):

Not applicable

Related Words/Test:

Serotonin, Serotonin metabolite, Chromogranin A

 

Test:Urine Trimethylamine
Other Test Request Name:

Trimethylamine

Test Composition:

Not Applicable

Intended Use:Not Applicable
Methodology:

SPME headspace technique in combination with gas chromatography mass spectrometry (GCMS).

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:

Not Applicable

Collection/Sample Container:

Plastik leak proof container

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

15 ML random Urine

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

Not Applicable

•   Refrigerated Temperature (2-8°C):

Not Applicable

•   Freezer Temperature (-20°C):

3 weeks

Transport Temperature:

(-18° C) Freezer Temp

Rejection Criteria:Not Applicable
Running Day:

Batch Running

Cut Off Time:

Friday 4 PM

TAT/Releasing of Results:

7 working weeks except Saturday, Sunday and Holidays

Reference Interval/Result Interpretation:

Not Applicable

Limitations/Interferences:

Not Applicable

Frequently Asked Questions (FAQs):

Not Applicable

Related Words/Test:

Not Applicable

 

Test:Valproic Acid (CMIA)
Other Test Request Name:

• Depakene
• Depakote
• Stavzor
• Divalproex sodium
• Valproate sodium

Test Composition:

Not applicable

Intended Use:

The measurements obtained are used in monitoring levels of valproic acid to help ensure appropriate therapy. Valproic acid is used alone or with other medications to treat certain types of seizures. Valproic acid is also used to treat mania (episodes of frenzied, abnormally excited mood) in people with bipolar disorder (manic-depressive disorder; a disease that causes episodes of depression, episodes of mania, and other abnormal moods). It is also used to prevent migraine headaches, but not to relieve headaches that have already begun. Valproic acid is in a class of medications called anticonvulsants. It works by increasing the amount of a certain natural substance in the brain.

Methodology:

Chemiluminescent Microparticle Immunoassay (CMIA)

Laboratory Section:

Immunology

Special Instructions/Patient Preparations:

• Collect as trough specimen (generally 30 minutes before the next dose) unless specified by the requesting physician.
• Provide date and time of last Valproic Acid intake and sample collection. This information is important to help requesting clinicians interpret the result.

Collection/Sample Container:

Red tube (DO NOT USE GOLD TUBE or any other type of tube with gel separator)

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

1-3 mL Serum
 

Alternative Specimen and Volume Requirement:

1-3 mL Plasma (Li-heparin, K-EDTA, Na-Heparin, Na-EDTA)

Specimen Stability  
•   Room Temperature (15-25°C):

2 Days

•   Refrigerated Temperature (2-8°C):

7 Days

•   Freezer Temperature (-20°C):

3 Months (5 Freeze-Thaw cycles)

Transport Temperature:

Transport specimen at 2°C – 8 °C (with cold packs)

Rejection Criteria:
  • Hemolyzed specimen
  • Markedly lipemic specimen
  • Specimen stored/transported outside the temperature range
  • Quantity not sufficient
  • Improperly labeled specimens
  • Samples collected in collection tube with gel separator
Running Day:

Tuesday, Friday

Cut Off Time:

Monday to Saturday: 10:00 PM

Sunday: 6:00PM

TAT/Releasing of Results:

ROUTINE (on running days)
• 4 hours after receipt of specimen/ arrival of messenger
STAT (on running days only)
• 2½ hours from extraction/messenger arrival

Reference Interval/Result Interpretation:

346.50-693.00 umol/L

Limitations/Interferences:

• Plasma samples from different anti-coagulant tube types should not be used interchangeably for monitoring valproic acid. Use of citrate should be performed only when the blood is collected in a full tube so as not incur a dilution effect.
• Specimens from patients who have received preparations of mouse monoclonal antibodies for diagnosis or therapy may contain human anti-mouse antibodies Such specimen may show either falsely elevated or depressed values when tested with this assay that employ mouse monoclonal antibodies.
•Heterophilic antibodies in human serum can react with reagent immunoglobulins, interfering with in vitro immunoassays.

Frequently Asked Questions (FAQs):

Not applicable

Related Words/Test:

TDM, Therapeutic Drug Monitoring, Anti convulsant

 

Test:Vancomycin
Other Test Request Name:

Not applicable

Test Composition:

Not applicable

Intended Use:

Vancomycin is an antibiotic. When taken by mouth it fights bacteria in the intestines used to treat an infection of the intestines caused by Clostridium difficile, which can cause watery or bloody diarrhea. It is also used to treat staph infections that can cause inflammation of the colon and small intestines.

Methodology:

Homogeneous Enzyme Immunoassay

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:
  • No patient preparation necessary.
  • Note date and time of last intake
  • Note last dosage
  • Note date and time of extraction
Collection/Sample Container:

Red tube (DO NOT USE GOLD TUBE or any other type of tube with gel separator)

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

3 mL Serum

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

72 Hours

•   Refrigerated Temperature (2-8°C):

7 Days

•   Freezer Temperature (-20°C):

Not applicable

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:
  • Hemolyzed specimen
  • Markedly lipemic specimen
  • Exceeded sample stability requirement
  • Quantity not sufficient
  • Improperly labeled specimen
  • Improper collection tube used
Running Day:

Monday to Friday

Cut Off Time:

Monday to Friday, 7:00 AM

TAT/Releasing of Results:

After 3 days (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Available upon request

Limitations/Interferences:

None specified.

Frequently Asked Questions (FAQs):

Not applicable

Related Words/Test:

Antibiotic, treat bacterial infections

 

Test:Varicella IgG
Other Test Request Name:

• Chicken Pox Test
• Varicella Zoster Virus Test
• Shingles Test

•Varicella Antibody IgG

Test Composition:

Not Applicable

Intended Use:

For determination of IgG-Class Antibodies to Varicella Zoster Virus in Human serum.

Methodology:

ENZYME LINKED IMMUNOSORBET ASSAY (ELISA)

Laboratory Section:

Serology

Special Instructions/Patient Preparations:

No patient preparation necessary.

Collection/Sample Container:

Red or Gold tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

2mL Serum

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

Not Specified

•   Refrigerated Temperature (2-8°C):

4 Days

•   Freezer Temperature (-20°C):

> 4 Days

Transport Temperature:

Transport specimen at 2 – 10 °C (with cold packs)

Rejection Criteria:

• Hemolyzed specimen
• Markedly lipemic specimen
• Exceeded sample stability requirement
• Specimen is stored and transported outside the required temperature
• Quantity not sufficient
• Improperly labeled specimens

Running Day:

Monday and Thursday (Batch running)

Cut Off Time:

Sunday, 6:00 PM; Wednesday 6:00 PM

TAT/Releasing of Results:

Monday,  6:00 PM; Friday 6:00 PM

Reference Interval/Result Interpretation:

NEGATIVE: <0.80 Index

EQUIVOCAL: between 0.80 and 1.20 Index

POSITIVE: >1.2 Index

Limitations/Interferences:

Not Applicable.

Frequently Asked Questions (FAQs):

Not Applicable

Related Words/Test:

Chickenpox, Shingles, Varicella Zoster Virus; VZV; Herpes Zoster, Varicella Zoster Virus Culture; Varicella Zoster Virus by PCR; Varicella Zoster Virus by DFA; Varicella Zoster Virus Antibodies, IgG and IgM

 

Test:Varicella IgM
Other Test Request Name:
  • Herpes zoster - IgM serology
  • Herpes ZOSTER
  • Anti-VZV antibodies - IgM
  • VZV - IgM serology
  • Varicella Zoster Virus - Serum

 

Test Composition:

Not applicable

Intended Use:

Useful in determining acute phase infection of varicella zoster virus. A virus which causes a highly contagious but usually benign disease of children which is characterized by vesicular skin lesions (chicken pox)

Methodology:

Chemiluminescence

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:

No patient preparation necessary.

Collection/Sample Container:

Red or Gold Tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

3 mL Serum

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

4 Days

•   Refrigerated Temperature (2-8°C):

7 Days

•   Freezer Temperature (-20°C):

Not applicable

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:

• Hemolyzed specimen
• Markedly lipemic specimen
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used

Running Day:

Batch Running

Cut Off Time:

Friday 4:00 PM

TAT/Releasing of Results:

2 weeks after cut-off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Available upon request

Limitations/Interferences:

None specified.

Frequently Asked Questions (FAQs):

Not applicable

Related Words/Test:

Varicella Zoster Virus, VZV, Herpes Zoster, Varicella Zoster Virus Culture, Varicella Zoster Virus by PCR, Varicella Zoster Virus by DFA, Varicella Zoster Virus Antibodies, IgG and IgM

 

Test:VIP (RIA)Temporarily Unavailable (currently not accepting samples)
Other Test Request Name:

Vasoactive Intestinal peptide

Test Composition:

Not applicable

Intended Use:

A polypeptide found at nerve endings in the gut which stimulates gastrointestinal motility and secretion. High levels associated with Verner-Morrison syndrome (which is due to a neurendocrine tumor in the pancreas).

Methodology:

Radio Immuno Assay (RIA)

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:
  • No patient preparation necessary.
  • Ask for  Atropin EDTA tube (Provided by Special Test Section)
  • Cetrifuge and separate plasma within 1 hour.
  • Send sample immediately to HPD Del Monte
Collection/Sample Container:

Atropin EDTA (provided by Special Test Section)

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

3 mL Plasma from Atropin EDTA tube

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

1 Hour

•   Refrigerated Temperature (2-8°C):

7 Days

•   Freezer Temperature (-20°C):

28 Days

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:
  • Exceeded sample stability requirement
  • Hemolyzed specimen
  • Quantity not sufficient
  • Improperly labeled specimen
  • Improper collection tube used


 

Running Day:

Batch Running

Cut Off Time:

Friday, 4:00 PM

TAT/Releasing of Results:

1 month after cut-off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Available upon request

Limitations/Interferences:

None specified.

Frequently Asked Questions (FAQs):

Not Applicable

Related Words/Test:

VIPoma, Verner–Morrison syndrome, endocrine tumor

 

Test:Viscosity (Serum)
Other Test Request Name:

Not Applicable

Test Composition:

Not Applicable

Intended Use:

High serum viscosity may be most commonly observed in patients with Waldenström's macroglobulinemia and multiple myeloma. Patients with high viscosity may have capillary occlusion, stasis hypoxia, and acidosis.

Methodology:

Kinematic Viscometer

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:

No patient preparation required.

Collection/Sample Container:

Gold or Red Top Tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

3 mL Serum

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

24 Hours

•   Refrigerated Temperature (2-8°C):

5 Days

•   Freezer Temperature (-20°C):

30 Days

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:
  • Insufficient volume
  • Improperly labeled sample
  • Markedly lipemic sample
  • Hemolyzed sample
  • Exceeded sample stability requirement
Running Day:

Batch Running.

Cut Off Time:

Monday, 12:00 PM

TAT/Releasing of Results:

2 weeks after cut off (excluding saturday, sunday and holidays).

Reference Interval/Result Interpretation:

Available Upon Request.

Limitations/Interferences:

None Applicable

Frequently Asked Questions (FAQs):

Q: What does viscosity mean?

A:Viscosity is a measure of a fluid's resistance to flow. It describes the internal friction of a moving fluid. A fluidwith large viscosity resists motion because its molecular makeup gives it a lot of internal friction.

Q: What is the cause of viscosity?

A:Viscosity is caused by friction within a fluid. It is the result of intermolecular forces between particles within a fluid.

Q: How does thickness relate to viscosity?

A:Every fluid material has a basic physical property, called its viscosity, which is a measure of its resistance to flow. The “thicker” a fluid is, the higher is its viscosity, because of its greater resistance to flow.

 

Related Words/Test:

Not Applicable

 

Test:Vitamin A (Retinol) HPLC
Other Test Request Name:
  • Retinol
  • Axerophtol
Test Composition:

Not Applicable

Intended Use:

This test is used for the investigation of deficiency due to malabsorption or rbp deficiency, and for chronic poisoning (which is fairly common).

Methodology:

High Performance Liquid Chromatography (HPLC)

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:
  • No patient preparation necessary.
  • Sample should keep away from light.
Collection/Sample Container:

2 pcs of 4mL Green Top (Sodium/Lithium Heparin)

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

4 mL Plasma Heparin

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

24 Hours

•   Refrigerated Temperature (2-8°C):

7 Days

•   Freezer Temperature (-20°C):

28 Days

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:
  • Hemolyzed specimen
  • Markedly lipemic specimen
  • Exceeded sample stability requirement
  • Quantity not sufficient
  • Improperly labeled specimen
  • Improper collection tube used
Running Day:

Batch Running

Cut Off Time:

Friday, 12:00 PM

TAT/Releasing of Results:

2 weeks after cut-off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Available upon request

Limitations/Interferences:

None Specified.

Frequently Asked Questions (FAQs):

Q: Why Get Tested?

A:To detect vitamin A deficiency or toxicity

Q: When To Get Tested?

A:When you have symptoms suggesting a vitamin A deficiency or excess, or are at risk for a deficiency

Q: How it is used?

A:Vitamin A testing is used to help diagnose vitamin A deficiency in people with symptoms, such as night blindness, or in people with diseases that impair intestinal absorption of nutrients and who are at risk of vitamin A deficiency. Testing for this purpose is not common because vitamin A deficiencies are rare in the United States. Sometimes testing may be used to detect toxic levels caused by ingestion of large amounts of vitamin A.

Vitamin A is an essential nutrient required for healthy vision, skin growth and integrity, bone formation, immune function, and embryonic development. It is required to produce photoreceptors in the eyes and to maintain the lining of the surface of the eyes and other mucous membranes.

The body cannot make vitamin A and must rely on dietary sources of vitamin A. Meat sources provide vitamin A (as retinol), while vegetable and fruit sources provide carotene (a substance that can be converted into vitamin A by the liver). Deficiencies in vitamin A can impair night vision, cause eye damage, and in severe cases, lead to blindness. Acute or chronic excesses of vitamin A can be toxic, cause a range of symptoms, and sometimes lead to birth defects.

Q: When is it ordered?

A:A vitamin A test may be ordered when a person has signs and symptoms that suggest a vitamin A deficiency or has general malnutrition. These signs and symptoms include:

  • Night blindness
  • Dry eyes, skin, and hair
  • Ulcers and damage to the cornea, the outermost layer of the front of the eye that covers the iris and pupil
  • Skin thickening and lesions
  • Grayish spots on eye lining (Bitot spots)
  • Repeated infections
  • Anemia
Related Words/Test:

Retinol, Chronic lung disease called cystic fibrosis

 

Test:Vitamin B1 (HPLC)
Other Test Request Name:
  • Aneurin
  • Thiamin
  • Vitamin B1 - Thiamin
     
Test Composition:

Not Applicable

Intended Use:

Thiamine deficiency usually occurs in alcoholics or as a result of inadequate intake (including non-supplemented parenteral alimentation). Inadequate intake causes beri-beri but this disease is almost entirely confined to South-East Asia.

Methodology:

High Performance Liquid Chromatography (HPLC)

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:
  • No patient preparation necessary.
  • Store away from light.
Collection/Sample Container:

EDTA or Violet Tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

3 pcs of 4 mL EDTA whole blood (kept away from light)

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

24 Hours

•   Refrigerated Temperature (2-8°C):

5 Days

•   Freezer Temperature (-20°C):

28 Days

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:
  • Exceeded sample stability requirement
  • Quantity not sufficient
  • Improperly labeled specimen
  • Improper collection tube used
  • Clotted Sample
  • Over-filled or Under-filled tube
  • Recived unprotected from light (no cover)
Running Day:

Batch Running

Cut Off Time:

Friday, 12:00 PM

TAT/Releasing of Results:

3 weeks after cut-off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Available upon request

Limitations/Interferences:

None Specified.

Frequently Asked Questions (FAQs):

Not Applicable

Related Words/Test:

Vitamin B complex, B1 (thiamine or thiamin), B2 (riboflavin), B3 (niacin), B5 (pantothenic acid), B6 (pyridoxal phosphate), B7 (biotin)

 

Test:Vitamin B12 (ECLIA)
Other Test Request Name:

• Cyanocobalamin
• Cobalamin

Test Composition:

Not applicable

Intended Use:

• For investigation of macrocytic anemia
• For work-up of deficiencies seen in megaloblastic anemias
• For Assistance in diagnosis of CNS (Central Nervous System) disorders
• For evaluation of alcoholism
• For evaluation of mal-absorption syndromes

Methodology:

Electrochemiluminescence Immunoassay (ECLIA)

Laboratory Section:

Immunology

Special Instructions/Patient Preparations:

•       8-12 hours fasting.

Note:

  Prolonged fasting is highly discouraged since various metabolic changes associated with starvation may begin to develop. Water intake is acceptable.

Note in clinical info if patient drank more than 4 glasses of water during fasting period, ask patient for medical history as this is rather unusual.

Note in clinical info in case patient overfasts and insists on proceeding test.

Samples should not be taken from patients receiving therapy with high biotin doses (i.e. > 5 mg/day) until at least 8 hours following the last biotin administration.

Protect sample from light by wrapping the sample tube with aluminum foil or carbon paper. Send sample to HPD Main lab immediately.

NOTE:Put label both on tube and on top of wrapping material to avoid mislabeling and unnecessary exposure of sample to light.

Collection/Sample Container:

Red or Gold tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

1-3 mL Serum

Alternative Specimen and Volume Requirement:

1-3 Plasma (Li- or Na-heparin, K3-EDTA)

Specimen Stability  
•   Room Temperature (15-25°C):

Not specified

•   Refrigerated Temperature (2-8°C):

2 Days

•   Freezer Temperature (-20°C):

2 Months (Freeze only once)

Transport Temperature:

Transport specimen at 2°C – 8 °C (with cold packs)

Rejection Criteria:

• Hemolyzed specimen
• Markedly lipemic sample
• Exceeded sample stability requirement
• Quantity not sufficient
• Improperly labeled specimen
• Improper collection tube used

Running Day:

Tuesday and Friday

Cut Off Time:

Monday to Saturday: 10:00 PM

Sunday: 6:00PM

TAT/Releasing of Results:

ROUTINE (on running days)
• 4 hours after receipt of specimen/ arrival of messenger
STAT (on running days only)
• 2½ hours from extraction/messenger arrival

 

Reference Interval/Result Interpretation:

145.00~569.00 pmol/L

 

Limitations/Interferences:

• Samples should not be taken from patients receiving therapy with high biotin doses (i.e. > 5 mg/day) until at least 8 hours following the last biotin administration.

• In rare cases, interference due to extremely high titers of antibodies to analyte-specific antibodies, streptavidin or ruthenium can occur.

• Samples with extremely high total protein concentrations (hyperproteinemia) are not suitable for use in this assay. Hyperproteinemia may be caused by, but not limited to, the following conditions: Lymphoma, bone marrow disorders such as multiple myeloma, monoclonal gammopathy of undetermined significance (MGUS), Waldenstrom macroglobulinemia, plasmocytoma, Amyloidosis. Respective samples may lead to the formation of protein gel in the assay cup, which may cause a run abort. The critical total protein concentration is dependent upon the individual sample composition.

Frequently Asked Questions (FAQs):

Not applicable

Related Words/Test:

Complete Blood Count, Methylmalonic Acid, Homocysteine, B Vitamins, Intrinsic Factor Antibody, Parietal Cell Antibody, Reticulocyte Count, Blood Smear, Folic Acid

 

Test:Vitamin B2 (Riboflavin)
Other Test Request Name:
  • Riboflavin
  • B2
Test Composition:

Not Applicable

Intended Use:

Vitamin B2 is involved in metabolism of fats, carbohydrtaes and protein. The clinical manifestations of deficiency are non-specific.Clinical manifestations include mucocutaneous lesions of the mouth and skin, corneal vascularization, anemia and personality changes.

Methodology:

Chromatography/Mass Spectrometry

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:
  • Fasting is required atleast 8 hours
  • Avoid Vitamin supplements for 24hrs prior to collection 
  • Sample should keep away from light. Transport tube wrapped in aluminum foil to protect from light
Collection/Sample Container:

EDTA tube  or Violet top

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

3 mL plasma(protected from light) 

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

2 Hours

•   Refrigerated Temperature (2-8°C):

24 Hours

•   Freezer Temperature (-20°C):

28 Days

Transport Temperature:

Transport specimen at - 20 °C (frozen)/ with dry ice (if more the 24 hrs travel)

Rejection Criteria:
  • Exceeded sample stability requirement
  • Quantity not sufficient
  • Improperly labeled specimen
  • Improper collection tube used
  • Not protected from light
  • Hemolyzed specimen
  • Markedly lipemic specimen
  • Exceeded sample stability requirement
  • Quantity not sufficient
  • Improperly labeled specimen
  • Improper collection tube used
     
Running Day:

Batch Running

Cut Off Time:

Monday, 12:00 PM

 

TAT/Releasing of Results:

2 weeks after cut-off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Available upon request.

Limitations/Interferences:

None Specified

Frequently Asked Questions (FAQs):

Not Applicable

Related Words/Test:

Not Applicable

 

Test:Vitamin B6 (HPLC)
Other Test Request Name:

• Pyridoxal
• Pyridoxamine
• Pyridoxine
• Pyridoxal phosphate

Test Composition:

Not applicable

Intended Use:

This test is useful in the diagnosis of deficiency which reveals as mucocutaneous, neurological, psychiatric and hematological disturbances. Deficiency can arise in chronic alcoholics, pregnant and breast-feeding women and in patients on non-supplemented parenteral alimentation or hemodialysis.

Methodology:

High Performance Liquid Chromatography (HPLC)

Laboratory Section:

Special Test

Special Instructions/Patient Preparations:

No patient preparation necessary.

Collection/Sample Container:

EDTA or Violet Tube

Specimen and Volume Requirement
Note:Follow tube manufacturer recommendation.		:

3 pcs of 4 mL EDTA whole blood

Alternative Specimen and Volume Requirement:

Not Applicable

Specimen Stability  
•   Room Temperature (15-25°C):

72 Hours

•   Refrigerated Temperature (2-8°C):

7 Days

•   Freezer Temperature (-20°C):

28 Days

Transport Temperature:

Transport specimen at 2 – 8 °C (with cold packs)

Rejection Criteria:
  • Exceeded sample stability requirement
  • Quantity not sufficient
  • Improperly labeled specimen
  • Improper collection tube used
  • Clotted Sample
  • Recived unprotected from light (no cover)
  • Over-filled or Under-filled tube
Running Day:

Batch Running

Cut Off Time:

Friday, 4:00 PM

TAT/Releasing of Results:

3 weeks after cut-off (excluding Saturdays, Sundays and Holidays)

Reference Interval/Result Interpretation:

Available upon request

Limitations/Interferences:

None specified.

Frequently Asked Questions (FAQs):

Not Applicable

Related Words/Test:

Vitamin B complex, B1 (thiamine or thiamin), B2 (riboflavin), B3 (niacin), B5 (pantothenic acid), B6 (pyridoxal phosphate), B7 (biotin)